兰州大学机构库 >化学化工学院
Microwave-assisted derivatization for fast and efficient analysis of saccharides on disposable microchips
Guo, JX; Yang, SH; Peng, XL; Li, FY(李风云); Zhou, L(周雷); Pu, QS(蒲巧生); Pu, QS (reprint author), Lanzhou Univ, Dept Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China.
收录类别SCIE ; EI
2014
发表期刊RSC ADVANCES
卷号4期号:90页码:49190-49197
出版者ROYAL SOC CHEMISTRY
出版地CAMBRIDGE
摘要For rapid and low-cost determination of saccharides with microchip electrophoresis, a domestic microwave oven was used to perform the derivatization of saccharides with 8-aminonaphthalene-1,3,6-trisulfonate (ANTS) for laser-induced fluorescence detection with a 405 nm laser diode. The influence of the reaction conditions was systematically examined. Derivatization of xylose, glucose and lactose was fulfilled within 3 min under the optimized condition. With appropriate selection of the separation medium, these model analytes could be resolved within 30 s in borate solutions containing hydroxypropyl cellulose using disposable cyclic olefin copolymer microchips. Theoretical plate numbers of 1.0 x 10(6) m(-1) and limits of detection of 0.15-0.23 mu M were achieved. Peak areas were linear up to 1000 mM for all analytes. The applicability of the proposed method was verified by the determination of glucose in human urine and serum. Separation of oligosaccharides from konjac powder was also demonstrated. The domestic microwave oven, the cheap laser diode and disposable microchips used here make the method an economical way for rapid analysis of saccharides.
作者部门[Guo, Jinxiu;
Yang, Shenghong;
Peng, Xianglu;
Li, Fengyun;
Zhou, Lei;
Pu, Qiaosheng] Lanzhou Univ, Dept Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China
学科领域Chemistry
所属项目编号National Natural Science Foundation of China [21175062, 21105040] ; Specialized Research Fund for the Doctoral Program of Higher Education [20110211120010] ; Fundamental Research Funds for the Central Universities [lzujbky-2013-89]
资助项目国家自然科学基金项目 ; 高等学校博士学科点专项科研基金 ; 中央高校基本科研业务费专项资金
项目资助者NSFC ; MOE ; LZU
DOI10.1039/c4ra07934e
ISSN2046-2069
文章类型Article
语种英语
WOS记录号WOS:000343711000057
EI记录号20144300122737
第一机构
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文献类型期刊论文
条目标识符http://ir.lzu.edu.cn/handle/262010/103296
Collection化学化工学院
通讯作者Pu, QS (reprint author), Lanzhou Univ, Dept Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China.
Recommended Citation:
GB/T 7714
Guo, JX,Yang, SH,Peng, XL,et al. Microwave-assisted derivatization for fast and efficient analysis of saccharides on disposable microchips[J]. RSC ADVANCES,2014,4(90):49190-49197.
APA Guo, JX.,Yang, SH.,Peng, XL.,Li, FY.,Zhou, L.,...&Pu, QS .(2014).Microwave-assisted derivatization for fast and efficient analysis of saccharides on disposable microchips.RSC ADVANCES,4(90),49190-49197.
MLA Guo, JX,et al."Microwave-assisted derivatization for fast and efficient analysis of saccharides on disposable microchips".RSC ADVANCES 4.90(2014):49190-49197.
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