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Microwave-assisted derivatization for fast and efficient analysis of saccharides on disposable microchips
Guo, JX; Yang, SH; Peng, XL; Li, FY(李风云); Zhou, L(周雷); Pu, QS(蒲巧生); Pu, QS (reprint author), Lanzhou Univ, Dept Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China.
Indexed BySCIE ; EI
2014
Source PublicationRSC ADVANCES
Volume4Issue:90Pages:49190-49197
PublisherROYAL SOC CHEMISTRY
Publication PlaceCAMBRIDGE
AbstractFor rapid and low-cost determination of saccharides with microchip electrophoresis, a domestic microwave oven was used to perform the derivatization of saccharides with 8-aminonaphthalene-1,3,6-trisulfonate (ANTS) for laser-induced fluorescence detection with a 405 nm laser diode. The influence of the reaction conditions was systematically examined. Derivatization of xylose, glucose and lactose was fulfilled within 3 min under the optimized condition. With appropriate selection of the separation medium, these model analytes could be resolved within 30 s in borate solutions containing hydroxypropyl cellulose using disposable cyclic olefin copolymer microchips. Theoretical plate numbers of 1.0 x 10(6) m(-1) and limits of detection of 0.15-0.23 mu M were achieved. Peak areas were linear up to 1000 mM for all analytes. The applicability of the proposed method was verified by the determination of glucose in human urine and serum. Separation of oligosaccharides from konjac powder was also demonstrated. The domestic microwave oven, the cheap laser diode and disposable microchips used here make the method an economical way for rapid analysis of saccharides.
Department[Guo, Jinxiu;
Yang, Shenghong;
Peng, Xianglu;
Li, Fengyun;
Zhou, Lei;
Pu, Qiaosheng] Lanzhou Univ, Dept Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China
Subject AreaChemistry
Project NumberNational Natural Science Foundation of China [21175062, 21105040] ; Specialized Research Fund for the Doctoral Program of Higher Education [20110211120010] ; Fundamental Research Funds for the Central Universities [lzujbky-2013-89]
Funding Project国家自然科学基金项目 ; 高等学校博士学科点专项科研基金 ; 中央高校基本科研业务费专项资金
Funding OrganizationNSFC ; MOE ; LZU
DOI10.1039/c4ra07934e
ISSN2046-2069
SubtypeArticle
Language英语
WOS IDWOS:000343711000057
EI ID20144300122737
First Inst
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.lzu.edu.cn/handle/262010/103296
Collection化学化工学院
Corresponding AuthorPu, QS (reprint author), Lanzhou Univ, Dept Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China.
Recommended Citation
GB/T 7714
Guo, JX,Yang, SH,Peng, XL,et al. Microwave-assisted derivatization for fast and efficient analysis of saccharides on disposable microchips[J]. RSC ADVANCES,2014,4(90):49190-49197.
APA Guo, JX.,Yang, SH.,Peng, XL.,Li, FY.,Zhou, L.,...&Pu, QS .(2014).Microwave-assisted derivatization for fast and efficient analysis of saccharides on disposable microchips.RSC ADVANCES,4(90),49190-49197.
MLA Guo, JX,et al."Microwave-assisted derivatization for fast and efficient analysis of saccharides on disposable microchips".RSC ADVANCES 4.90(2014):49190-49197.
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