生命科学学院机构知识库

Institutional Repository, School of Life Sciences

 

兰州大学机构库  > 生命科学学院  > 期刊论文
题名: Curcumin-induced histone hypoacetylation: The role of reactive oxygen species
作者: Kang, JH ; Chen, J ; Shi, YF ; Jia, R ; Zhang, YT
收录类别: SCIE ; MEDLINE ; BIOSIS
出版日期: 2005-04-15
刊名: BIOCHEMICAL PHARMACOLOGY
卷号: 69, 期号:8, 页码:1205-1213
英文摘要: Curcumin (Cur), a well-known dietary pigment derived from Curcuma longa, is a promising anticancer drug, but its in vivo target molecules remain to be clarified. Here we report that exposure of human hepatoma cells to Cur led to a significant decrease of histone acetylation. Histone acetyltransferase (HAT) and histone deacetylase (HDAC) are the enzymes controlling the state of histone acetylation in vivo. Cur treatment resulted in a comparable inhibition of histone acetylation in the absence or presence of trichostatin A (the specific HDAC inhibitor), and showed no effect on the in vitro activity of HDAC. In contrast, the domain negative of p300 (a most potent HAT protein) could block the inhibition of Cur on histone acetylation; and the Cur treatment significantly inhibited the HAT activity both in vivo and in vitro. Thus, it is HAT, but not HDAC that is involved in Cur-induced histone hypoacetylation. At the same time, exposure of cells to low or high concentrations of Cur diminished or enhanced the ROS generation, respectively. And the promotion of ROS was obviously involved in Cur-induced histone hypoacetylation, since Cur-caused histone acetylation and HAT activity decrease could be markedly diminished by the antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) or their combination, but not by their heat-inactivated forms. The data presented here prove that HAT is one of the in vivo target molecules of Cur; through inhibiting its activity, Cur induces histone hypoacetylation in vivo, where the ROS generation plays an important role. Considering the critical roles of histone acetylation in eukaryotic gene transcription and the involvement of histone hypoacetylation in the lose of cell viability caused by high concentrations of Cur, these results open a new door for us to further understand the molecular mechanism involved in the in vivo function of Cur. (c) 2005 Elsevier Inc. All rights reserved.
关键词: curcumin ; histone acetylation ; histone acetyltransferase (HAT) ; histone deacetylase (HDAC) ; reactive oxygen species (ROS) ; p300
作者部门: Lanzhou Univ, Inst Phys, Sch Life Sci, Lanzhou 730000, Peoples R China ; Gen Hosp Lanzhou, Dept Hematol, Lanzhou 730000, Peoples R China ; Chinese Acad Sci, Shanghai Inst Cell Biol, Shanghai 200031, Peoples R China
通讯作者: Kang, JH (reprint author), Lanzhou Univ, Inst Phys, Sch Life Sci, Lanzhou 730000, Peoples R China.
学科分类: Pharmacology & Pharmacy
文章类型: Article
语种: 英语
DOI: 10.1016/j.bcp.2005.01.014
ISSN号: 0006-2952
WOS记录号: WOS:000228426400009
PM记录号: 15794941
BIOSIS记录号: BIOSIS:PREV200510052668
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/103308
Appears in Collections:生命科学学院_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
000228426400009.pdf(254KB)----限制开放 联系获取全文
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Altmetrics Score
 
Google Scholar
Similar articles in Google Scholar
[Kang, JH]'s Articles
[Chen, J]'s Articles
[Shi, YF]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Kang, JH]‘s Articles
[Chen, J]‘s Articles
[Shi, YF]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Email:
Passwd
验 证:
换一张
Have you forgotten your password? Log In
Copyright © 2007-2017  兰州大学 - Feedback
Powered by CSpace