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兰州大学机构库  > 草地农业科技学院  > 期刊论文
题名: Protein-Binding Affinity of Leucaena Condensed Tannins of Differing Molecular Weights
作者: Huang, XD(黄晓东); Liang, JB; Tan, HY; Yahya, R; Long, RJ(龙瑞军); Ho, YW
收录类别: SCIE ; EI
出版日期: 2011-10-12
刊名: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷号: 59, 期号:19, 页码:10677-10682
出版者: AMER CHEMICAL SOC
出版地: WASHINGTON
英文摘要: Depending on their source, concentration, chemical structure, and molecular weight, condensed tannins (CTs) form insoluble complexes with protein, which could lead to ruminal bypass protein, benefiting animal production. In this study, CTs from Leuceana leucocephala hybrid were fractionated into five fractions by a size exclusion chromatography procedure. The molecular weights of the CT fractions were determined using Q-TOF LC-MS, and the protein-binding affinities of the respective CT fractions were determined using a protein precipitation assay with bovine serum albumin (BSA) as the standard protein. The calculated number-average molecular weights (M(n)) were 1348.6, 857.1, 730.1, 726.0, and 497.1, and b values (the b value represents the CT quantity that is needed to bind half of the maximum precipitable BSA) of the different molecular weight fractions were 0.381, 0.510, 0.580, 0.636, and 0.780 for fractions 1, 2, 3, 4, and 5, respectively. The results indicated that, in general, CTs of higher molecular weight fractions have stronger protein-binding affinity than those of lower molecular weights. However, the number of hydroxyl units within the structure of CT polymers also affects the protein-binding affinity.
关键词: condensed tannins ; molecular weight ; structure ; protein binding ; leucaena
作者部门: [Liang, Juan Boo] Univ Putra Malaysia, Inst Trop Agr, Lab Anim Prod, Serdang 43400, Malaysia ; [Huang, Xiao Dan ; Tan, Hui Yin ; Ho, Yin Wan] Univ Putra Malaysia, Inst Biosci, Lab Ind Biotechnol, Serdang 43400, Malaysia ; [Yahya, Rosiyah] Univ Malaya, Fac Sci, Dept Chem, Kuala Lumpur 50603, Malaysia ; [Huang, Xiao Dan ; Long, Ruijun] Lanzhou Univ, Int Ctr Tibetan Plateau Ecosyst Management, Lanzhou 730000, Peoples R China
通讯作者: Liang, JB (reprint author), Univ Putra Malaysia, Inst Trop Agr, Lab Anim Prod, Serdang 43400, Malaysia.
学科分类: Agriculture;Chemistry;Food Science & Technology
文章类型: Article
所属项目编号: Universiti Putra Malaysia [04/01/07/008RU]
语种: 英语
DOI: 10.1021/jf201925g
ISSN号: 0021-8561
WOS记录号: WOS:000295488000035
EI记录号: 20114114414262
第一机构:
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/113652
Appears in Collections:草地农业科技学院_期刊论文

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Recommended Citation:
Huang, XD,Liang, JB,Tan, HY,et al. Protein-Binding Affinity of Leucaena Condensed Tannins of Differing Molecular Weights[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2011,59(19):10677-10682.
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