兰州大学机构库 >生命科学学院
Structure determination, apoptosis induction, and telomerase inhibition of CFP-2, a novel lichenin from Cladonia furcata
Lin, X; Cai, YJ; Li, ZX; Chen, Q(陈强); Liu, ZL(刘中立); Wang, R(王锐); Wang, R (reprint author), Lanzhou Univ, Sch Life Sci, Dept Biochem & Mol Biol, Tianshui Rd 298, Lanzhou 730000, Gansu, Peoples R China.
2003-07-23
发表期刊BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
ISSN0304-4165
卷号1622期号:2页码:99-108
出版者ELSEVIER
摘要A great deal of experimental evidence has accumulated in the past several decades, suggesting that polysaccharides have wide bioactivities. Cladonia furcata polysaccharide, CFP-2, a water-soluble lichenin with a mean M-r 7.6 x 10(4), was first obtained by 0.25 M NaOH solution extraction, ethanol precipitation, DEAE-cellulose, and Sephadex G-200 column chromatography. Gas chromatography of acid hydrolyzate of CFP-2 suggested that it was composed of D-glucose, D-galactose, and D-mannose in the molar ratios of 8: 1: 1. Periodate oxidation, Smith degradation, IR, and NMR spectroscopy analysis revealed that CFP-2 had a backbone consisting of alpha-(1 --> 3) and alpha(1 --> 4)-linked D-glucopyranosyl residues substituted at O-6 with alpha-(1 --> 6)-linked D-galactopyranosyl residue and alpha-(1 --> 6)-linked D-mannopyranosyl residue. CFP-2 was able to reduce viability of cultured HL-60 and K562 cells. The antiproliferative properties of CFP-2 appeared to be attributable to its induction of apoptotic cell death as determined by ultrastructural change, intenucleosomal DNA fragmentation, and increased proportion of the subdiploid cell population. To elucidate molecular events in the apoptosis, protein expressions of Bcl-2, Bax, Fas, and FasL were measured by Western blotting using specific antibodies in HL-60 cells. The level of Bcl-2 remained largely unchanged, but the Bax, Fas, and FasL expression showed up-regulation. Moreover, the telomerase activity analyzed by TRAP-ELISA assay in HL-60 cells treated with CFP-2 decreased as compared with the untreated control cells. These results suggest that CFP-2 could have a possible cancer therapeutic potential. (C) 2003 Elsevier B.V. All rights reserved.
出版地AMSTERDAM
关键词Cladonia jarcata lichenin apoptosis telomerase HL-60 cell K562 cell
学科领域Biochemistry & Molecular Biology ; Biophysics
作者部门Lanzhou Univ, Sch Life Sci, Dept Biochem & Mol Biol, Lanzhou 730000, Gansu, Peoples R China;
Lanzhou Univ, Natl Lab Appl Organ Chem, Lanzhou 730000, Peoples R China
DOI10.1016/S0304-4165(03)00131-4
文章类型Article
收录类别SCIE ; PubMed ; MEDLINE ; BIOSIS
语种英语
WOS记录号WOS:000184499200005
PM记录号12880947
BIOSIS记录号BIOSIS:PREV200300465362
第一机构
Citation statistics
???java.ItemTag.Counts???35[WOS]   ???java.ItemTag.WOS.Record???     ???java.ItemTag.WOS.Related-Records???
文献类型期刊论文
条目标识符http://ir.lzu.edu.cn/handle/262010/114100
Collection生命科学学院
通讯作者Wang, R (reprint author), Lanzhou Univ, Sch Life Sci, Dept Biochem & Mol Biol, Tianshui Rd 298, Lanzhou 730000, Gansu, Peoples R China.
Recommended Citation:
GB/T 7714
Lin, X,Cai, YJ,Li, ZX,et al. Structure determination, apoptosis induction, and telomerase inhibition of CFP-2, a novel lichenin from Cladonia furcata[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2003,1622(2):99-108.
APA Lin, X.,Cai, YJ.,Li, ZX.,Chen, Q.,Liu, ZL.,...&Wang, R .(2003).Structure determination, apoptosis induction, and telomerase inhibition of CFP-2, a novel lichenin from Cladonia furcata.BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1622(2),99-108.
MLA Lin, X,et al."Structure determination, apoptosis induction, and telomerase inhibition of CFP-2, a novel lichenin from Cladonia furcata".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1622.2(2003):99-108.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Altmetrics Score
谷歌学术
谷歌学术Similar articles in Google Scholar
[Lin, X]'s Articles
[Cai, YJ]'s Articles
[Li, ZX]'s Articles
百度学术
百度学术Similar articles in Google Scholar
[Lin, X]'s Articles
[Cai, YJ]'s Articles
[Li, ZX]'s Articles
必应学术
必应学术Similar articles in Google Scholar
[Lin, X]'s Articles
[Cai, YJ]'s Articles
[Li, ZX]'s Articles
Related Copyright Policies
Null
???item.sidebar.baidu.bookmark-share???
???jsp.display-item.all??? (0)
[???jsp.display-item.idea???]
???jsp.display-item.comment-text2???
Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.