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题名: Structure determination, apoptosis induction, and telomerase inhibition of CFP-2, a novel lichenin from Cladonia furcata
作者: Lin, X; Cai, YJ; Li, ZX; Chen, Q(陈强); Liu, ZL(刘中立); Wang, R(王锐)
收录类别: SCIE ; PubMed ; MEDLINE ; BIOSIS
出版日期: 2003-07-23
刊名: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
卷号: 1622, 期号:2, 页码:99-108
出版者: ELSEVIER
出版地: AMSTERDAM
英文摘要: A great deal of experimental evidence has accumulated in the past several decades, suggesting that polysaccharides have wide bioactivities. Cladonia furcata polysaccharide, CFP-2, a water-soluble lichenin with a mean M-r 7.6 x 10(4), was first obtained by 0.25 M NaOH solution extraction, ethanol precipitation, DEAE-cellulose, and Sephadex G-200 column chromatography. Gas chromatography of acid hydrolyzate of CFP-2 suggested that it was composed of D-glucose, D-galactose, and D-mannose in the molar ratios of 8: 1: 1. Periodate oxidation, Smith degradation, IR, and NMR spectroscopy analysis revealed that CFP-2 had a backbone consisting of alpha-(1 --> 3) and alpha(1 --> 4)-linked D-glucopyranosyl residues substituted at O-6 with alpha-(1 --> 6)-linked D-galactopyranosyl residue and alpha-(1 --> 6)-linked D-mannopyranosyl residue. CFP-2 was able to reduce viability of cultured HL-60 and K562 cells. The antiproliferative properties of CFP-2 appeared to be attributable to its induction of apoptotic cell death as determined by ultrastructural change, intenucleosomal DNA fragmentation, and increased proportion of the subdiploid cell population. To elucidate molecular events in the apoptosis, protein expressions of Bcl-2, Bax, Fas, and FasL were measured by Western blotting using specific antibodies in HL-60 cells. The level of Bcl-2 remained largely unchanged, but the Bax, Fas, and FasL expression showed up-regulation. Moreover, the telomerase activity analyzed by TRAP-ELISA assay in HL-60 cells treated with CFP-2 decreased as compared with the untreated control cells. These results suggest that CFP-2 could have a possible cancer therapeutic potential. (C) 2003 Elsevier B.V. All rights reserved.
关键词: Cladonia jarcata ; lichenin ; apoptosis ; telomerase ; HL-60 cell ; K562 cell
作者部门: Lanzhou Univ, Sch Life Sci, Dept Biochem & Mol Biol, Lanzhou 730000, Gansu, Peoples R China ; Lanzhou Univ, Natl Lab Appl Organ Chem, Lanzhou 730000, Peoples R China
通讯作者: Wang, R (reprint author), Lanzhou Univ, Sch Life Sci, Dept Biochem & Mol Biol, Tianshui Rd 298, Lanzhou 730000, Gansu, Peoples R China.
学科分类: Biochemistry & Molecular Biology; Biophysics
文章类型: Article
语种: 英语
DOI: 10.1016/S0304-4165(03)00131-4
ISSN号: 0304-4165
WOS记录号: WOS:000184499200005
PM记录号: 12880947
BIOSIS记录号: BIOSIS:PREV200300465362
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/114100
Appears in Collections:生命科学学院_期刊论文

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Recommended Citation:
Lin, X,Cai, YJ,Li, ZX,et al. Structure determination, apoptosis induction, and telomerase inhibition of CFP-2, a novel lichenin from Cladonia furcata[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2003,1622(2):99-108.
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