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Structure determination, apoptosis induction, and telomerase inhibition of CFP-2, a novel lichenin from Cladonia furcata
Lin, X; Cai, YJ; Li, ZX; Chen, Q(陈强); Liu, ZL(刘中立); Wang, R(王锐); Wang, R (reprint author), Lanzhou Univ, Sch Life Sci, Dept Biochem & Mol Biol, Tianshui Rd 298, Lanzhou 730000, Gansu, Peoples R China.
Indexed BySCIE ; PubMed ; MEDLINE ; BIOSIS
2003-07-23
Source PublicationBIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
Volume1622Issue:2Pages:99-108
PublisherELSEVIER
Publication PlaceAMSTERDAM
AbstractA great deal of experimental evidence has accumulated in the past several decades, suggesting that polysaccharides have wide bioactivities. Cladonia furcata polysaccharide, CFP-2, a water-soluble lichenin with a mean M-r 7.6 x 10(4), was first obtained by 0.25 M NaOH solution extraction, ethanol precipitation, DEAE-cellulose, and Sephadex G-200 column chromatography. Gas chromatography of acid hydrolyzate of CFP-2 suggested that it was composed of D-glucose, D-galactose, and D-mannose in the molar ratios of 8: 1: 1. Periodate oxidation, Smith degradation, IR, and NMR spectroscopy analysis revealed that CFP-2 had a backbone consisting of alpha-(1 --> 3) and alpha(1 --> 4)-linked D-glucopyranosyl residues substituted at O-6 with alpha-(1 --> 6)-linked D-galactopyranosyl residue and alpha-(1 --> 6)-linked D-mannopyranosyl residue. CFP-2 was able to reduce viability of cultured HL-60 and K562 cells. The antiproliferative properties of CFP-2 appeared to be attributable to its induction of apoptotic cell death as determined by ultrastructural change, intenucleosomal DNA fragmentation, and increased proportion of the subdiploid cell population. To elucidate molecular events in the apoptosis, protein expressions of Bcl-2, Bax, Fas, and FasL were measured by Western blotting using specific antibodies in HL-60 cells. The level of Bcl-2 remained largely unchanged, but the Bax, Fas, and FasL expression showed up-regulation. Moreover, the telomerase activity analyzed by TRAP-ELISA assay in HL-60 cells treated with CFP-2 decreased as compared with the untreated control cells. These results suggest that CFP-2 could have a possible cancer therapeutic potential. (C) 2003 Elsevier B.V. All rights reserved.
KeywordCladonia jarcata lichenin apoptosis telomerase HL-60 cell K562 cell
DepartmentLanzhou Univ, Sch Life Sci, Dept Biochem & Mol Biol, Lanzhou 730000, Gansu, Peoples R China;
Lanzhou Univ, Natl Lab Appl Organ Chem, Lanzhou 730000, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Biophysics
DOI10.1016/S0304-4165(03)00131-4
ISSN0304-4165
SubtypeArticle
Language英语
WOS IDWOS:000184499200005
PMID 12880947
BIOSIS IDBIOSIS:PREV200300465362
First Inst
Citation statistics
Cited Times:35[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.lzu.edu.cn/handle/262010/114100
Collection生命科学学院
Corresponding AuthorWang, R (reprint author), Lanzhou Univ, Sch Life Sci, Dept Biochem & Mol Biol, Tianshui Rd 298, Lanzhou 730000, Gansu, Peoples R China.
Recommended Citation
GB/T 7714
Lin, X,Cai, YJ,Li, ZX,et al. Structure determination, apoptosis induction, and telomerase inhibition of CFP-2, a novel lichenin from Cladonia furcata[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2003,1622(2):99-108.
APA Lin, X.,Cai, YJ.,Li, ZX.,Chen, Q.,Liu, ZL.,...&Wang, R .(2003).Structure determination, apoptosis induction, and telomerase inhibition of CFP-2, a novel lichenin from Cladonia furcata.BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1622(2),99-108.
MLA Lin, X,et al."Structure determination, apoptosis induction, and telomerase inhibition of CFP-2, a novel lichenin from Cladonia furcata".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1622.2(2003):99-108.
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