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题名: A POSSIBLE CALCIUM-BINDING SITE IN D-1 PROTEIN - A FLUORESCENCE AND FTIR STUDY OF THE INTERACTION BETWEEN LANTHANIDES AND A SYNTHETIC PEPTIDE
作者: WANG, J; ZHANG, LX; Liu, ZL(刘中立); LIANG, HG; YANG, L; HU, XY
收录类别: SCIE ; PubMed ; BIOSIS
出版日期: 1995-06
刊名: Photosynthesis Research
卷号: 44, 期号:3, 页码:297-302
出版者: KLUWER
出版地: DORDRECHT
英文摘要: A peptide ranging from residue 229 to 240 of the D-1 protein of Photosystem (PS) II was synthesized and lanthanides were used as candidates of calcium. Fluorescence and FTIR spectroscopy were used to test the conformational adaptation after lanthanide additions. Fluorescence spectroscopy showed that the synthetic peptide provides lanthanide binding site, and that glutamic acids are involved in lanthanide binding. Resolution enhancement techniques were combined with band curve-fitting procedures to quantitate the FTIR spectral information from the amide 1 bands. The relative areas of these component bands indicate that lanthanide induced a substantial decrease in the amount of unordered structure and turns, while a corresponding increase in the amount of a-helix and 'open loop' was also observed. This indicates that a relatively compact structure of the synthetic peptide is formed if lanthanides are applied. The results may reflect on the physiological and biochemical function of calcium in PS II, including preventing D-1 from trypsin digestion.
关键词: CALCIUM BINDING SITE ; CONFORMATION ; D-1 FRAGMENT ; LANTHANIDES
通讯作者: WANG, J (reprint author), LANZHOU UNIV,DEPT BIOL,NATL LAB APPL ORGAN CHEM,LANZHOU 730000,PEOPLES R CHINA.
学科分类: Plant Sciences
文章类型: Article
语种: 英语
DOI: 10.1007/BF00048603
ISSN号: 0166-8595
WOS记录号: WOS:A1995RT09200008
PM记录号: 24307100
BIOSIS记录号: BCI:BCI199598523594
IR记录号: BCI:BCI199598523594
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/115599
Appears in Collections:生命科学学院_期刊论文

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A POSSIBLE CALCIUM-BINDING SITE IN D-1 PROTEIN - A FLUORESCENCE AND FTIR STUDY OF THE INTERACTION BETWEEN LANTHANIDES AND A SYNTHETIC PEPTIDE.pdf(628KB)----限制开放 联系获取全文

Recommended Citation:
WANG, J,ZHANG, LX,LIU, ZL,et al. A POSSIBLE CALCIUM-BINDING SITE IN D-1 PROTEIN - A FLUORESCENCE AND FTIR STUDY OF THE INTERACTION BETWEEN LANTHANIDES AND A SYNTHETIC PEPTIDE[J]. Photosynthesis Research,1995,44(3):297-302.
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