兰州大学机构库 >生命科学学院
A POSSIBLE CALCIUM-BINDING SITE IN D-1 PROTEIN - A FLUORESCENCE AND FTIR STUDY OF THE INTERACTION BETWEEN LANTHANIDES AND A SYNTHETIC PEPTIDE
WANG, J; ZHANG, LX; Liu, ZL(刘中立); LIANG, HG; YANG, L; HU, XY; WANG, J (reprint author), LANZHOU UNIV,DEPT BIOL,NATL LAB APPL ORGAN CHEM,LANZHOU 730000,PEOPLES R CHINA.
1995-06
发表期刊Photosynthesis Research
ISSN0166-8595
卷号44期号:3页码:297-302
出版者KLUWER
摘要A peptide ranging from residue 229 to 240 of the D-1 protein of Photosystem (PS) II was synthesized and lanthanides were used as candidates of calcium. Fluorescence and FTIR spectroscopy were used to test the conformational adaptation after lanthanide additions. Fluorescence spectroscopy showed that the synthetic peptide provides lanthanide binding site, and that glutamic acids are involved in lanthanide binding. Resolution enhancement techniques were combined with band curve-fitting procedures to quantitate the FTIR spectral information from the amide 1 bands. The relative areas of these component bands indicate that lanthanide induced a substantial decrease in the amount of unordered structure and turns, while a corresponding increase in the amount of a-helix and 'open loop' was also observed. This indicates that a relatively compact structure of the synthetic peptide is formed if lanthanides are applied. The results may reflect on the physiological and biochemical function of calcium in PS II, including preventing D-1 from trypsin digestion.
出版地DORDRECHT
关键词CALCIUM BINDING SITE CONFORMATION D-1 FRAGMENT LANTHANIDES
学科领域Plant Sciences
DOI10.1007/BF00048603
文章类型Article
收录类别SCIE ; PubMed ; BIOSIS
语种英语
WOS记录号WOS:A1995RT09200008
PM记录号24307100
BIOSIS记录号BCI:BCI199598523594
IR记录号BCI:BCI199598523594
第一机构
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文献类型期刊论文
条目标识符http://ir.lzu.edu.cn/handle/262010/115599
Collection生命科学学院
通讯作者WANG, J (reprint author), LANZHOU UNIV,DEPT BIOL,NATL LAB APPL ORGAN CHEM,LANZHOU 730000,PEOPLES R CHINA.
Recommended Citation:
GB/T 7714
WANG, J,ZHANG, LX,LIU, ZL,et al. A POSSIBLE CALCIUM-BINDING SITE IN D-1 PROTEIN - A FLUORESCENCE AND FTIR STUDY OF THE INTERACTION BETWEEN LANTHANIDES AND A SYNTHETIC PEPTIDE[J]. Photosynthesis Research,1995,44(3):297-302.
APA WANG, J.,ZHANG, LX.,LIU, ZL.,LIANG, HG.,YANG, L.,...&WANG, J .(1995).A POSSIBLE CALCIUM-BINDING SITE IN D-1 PROTEIN - A FLUORESCENCE AND FTIR STUDY OF THE INTERACTION BETWEEN LANTHANIDES AND A SYNTHETIC PEPTIDE.Photosynthesis Research,44(3),297-302.
MLA WANG, J,et al."A POSSIBLE CALCIUM-BINDING SITE IN D-1 PROTEIN - A FLUORESCENCE AND FTIR STUDY OF THE INTERACTION BETWEEN LANTHANIDES AND A SYNTHETIC PEPTIDE".Photosynthesis Research 44.3(1995):297-302.
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