兰州大学机构库 >生命科学学院
Purification and partial characterization of a protease associated with photosystem II particles
Zhang, LX; Wang, J; Wen, JQ; Liang, HG; Du, LF; Zhang, LX (reprint author), LANZHOU UNIV,DEPT BIOL,LANZHOU 730000,PEOPLES R CHINA.
收录类别SCIE ; BIOSIS
1995-12
发表期刊Physiologia Plantarum
卷号95期号:4页码:591-595
出版者MUNKSGAARD INT PUBL LTD
出版地COPENHAGEN
摘要A protease was extracted with 1 M NaCl from spinach (Spinacia oleracea L.) photosystem II (PSII) particles and purified through gel filtration and anion-exchange chromatography. SDS-polyacrylamide gel electrophoresis of the protease revealed a polypeptide with a molecular mass of 43 kDa. The activity of the purified protease was assayed using a 24 kDa water-soluble protein as substrate, visualized through SDS-PAGE. The protease even remained active in the presence of 0.1 and 0.2 M NaCl, although the degradation pattern changed, which indicated that the protease was different from that reported earlier by another group. The presence of 0.3 M NaCl was shown to Ire inhibitory. The protease was inhibited by 1,10-phenanthroline and EGTA-NaOH (pH 7.0), indicating that the metal ions are essential for activity and that the enzyme is a metal-protease. FTIR spectroscopy was used to examine the conformationally sensitive amide I' bands of the protease. The protease was observed to undergo spectroscopic changes that reflect the conformational changes that take place when Ca2+ is bound, which further confirms that the protease is a metal-protease.
关键词enzyme activity FTIR PSII particles protease 24-kDa protein SDS-PAGE
作者部门SICHUAN UNIV,DEPT BIOL,CHENGDU 610064,PEOPLES R CHINA
学科领域Plant Sciences
DOI10.1034/j.1399-3054.1995.950413.x
ISSN0031-9317
文章类型Article
语种英语
WOS记录号WOS:A1995TQ84100013
BIOSIS记录号BCI:BCI199698686115
IR记录号BCI:BCI199698686115
第一机构
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文献类型期刊论文
条目标识符http://ir.lzu.edu.cn/handle/262010/115994
Collection生命科学学院
通讯作者Zhang, LX (reprint author), LANZHOU UNIV,DEPT BIOL,LANZHOU 730000,PEOPLES R CHINA.
Recommended Citation:
GB/T 7714
Zhang, LX,Wang, J,Wen, JQ,et al. Purification and partial characterization of a protease associated with photosystem II particles[J]. Physiologia Plantarum,1995,95(4):591-595.
APA Zhang, LX,Wang, J,Wen, JQ,Liang, HG,Du, LF,&Zhang, LX .(1995).Purification and partial characterization of a protease associated with photosystem II particles.Physiologia Plantarum,95(4),591-595.
MLA Zhang, LX,et al."Purification and partial characterization of a protease associated with photosystem II particles".Physiologia Plantarum 95.4(1995):591-595.
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