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题名: Effect of TNF-alpha and IFN-alpha on the proliferation and cytotoxicity of lymphokine-activated killer in patients with bladder cancer
作者: Wang, ZP(王志平); Cheng, YR; Zheng, RL(郑荣梁); Qin, DS; Liu, GD
收录类别: SCIE ; BIOSIS
出版日期: 1997-03
刊名: CHINESE MEDICAL JOURNAL
卷号: 110, 期号:3, 页码:180-183
英文摘要: Objective To investigate the effects of interleukin-2 (IL-2) combined with either tumor necrosis factor-alpha (TNF-alpha) or alpha-interferon (IFN-alpha) on the proliferation and cytolysis to bladder tumor cells of lymphokine-activated killer (LAK) cells in patients with bladder cancer. Methods LAK cells were generated by ficoll-paque density-centrifugation from 21 patients with bladder cancer and cultured in medium containing IL-2. LAK cell proliferation was assayed in the presence of various concentrations of either TNF-alpha or IFN-alpha by cell count in 96-well plates. Bladder cancer cell lines BIU-87 and EJ were cultured as target cells and the cytotoxicity of LAK cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. Results The proliferation of LAK cells induced by IL-2 was enhanced by TNF-alpha in a dose-responsive fashion. The direct growth support for the LAK cells was also observed with IFN-alpha at the concentration of 1000 U/ml after 48 hours of culture. TNF-alpha (5000 U/ml) resulted in an increase in the cytotoxicity of LAK cells to BIU-87 and EJ cells. However, the change of cytotoxicity of LAK cells treated with IFN-alpha was not statistically significant. Conclusions TNF-alpha and IFN-alpha enhance the proliferation and activation of LAK cells and influence their antitumor cytotoxicity in patients with bladder cancer.
作者部门: LANZHOU MED COLL, AFFILIATED HOSP 2, INST UROL, LANZHOU 730030, PEOPLES R CHINA ; LANZHOU UNIV, DEPT BIOL, LANZHOU 730000, PEOPLES R CHINA
学科分类: General & Internal Medicine
文章类型: Article
语种: 英语
ISSN号: 0366-6999
WOS记录号: WOS:A1997WU89000005
BIOSIS记录号: BCI:BCI199799646981
IR记录号: BCI:BCI199799646981
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/116007
Appears in Collections:生命科学学院_期刊论文

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Recommended Citation:
Wang, ZP,Cheng, YR,Zheng, RL,et al. Effect of TNF-alpha and IFN-alpha on the proliferation and cytotoxicity of lymphokine-activated killer in patients with bladder cancer[J]. CHINESE MEDICAL JOURNAL,1997,110(3):180-183.
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