兰州大学机构库 >生命科学学院
Synthesis and DNA-binding ability of Sp1 protein zinc finger domain and its peptidomimetics
Wang, R(王锐); Ni, JM(倪京满); Hu, XY; Ma, YP; Li, XQ; Yang, DJ; Dong, SL(董守良); Yang, XW; Pan, XF(潘鑫复)
收录类别SCIE ; PubMed ; BIOSIS
1997-10
发表期刊SCIENCE IN CHINA SERIES C-LIFE SCIENCES
卷号40期号:5页码:518-523
期刊主办单位中国科学院
出版者SCIENCE CHINA PRESS
出版地BEIJING
摘要The second zinc finger fragment of Sp1 (Sp1-ZF2), its mutant (Sp1-ZF2/HT. E-20 --> H, R-23 --> T), and two mimic analogues (ZF20 and ZF15) were synthesized by stepwise solid phase technique. The CD spectra and UV-visible spectrum with CoCl2 indicated that the formation of zinc finger structure was affected not only by the hydrophobic amino acids but also by the change of the distance between Cys and His. Gel-retardation electrophoresis assays indicated that the Glu and Arg residues are very important for recognition. A single zinc finger like Sp1-ZF2 is able to bind DNA sequence specifically.
关键词zinc finger domain Sp1 protein solid phase peptide synthesis sequence specifically oligonucleotide
作者部门LANZHOU UNIV,DEPT BIOL,STATE KEY LAB APPL ORGAN CHEM,LANZHOU 730000,PEOPLES R CHINA
学科领域Life Sciences & Biomedicine - Other Topics
ISSN1006-9305
文章类型Article
语种英语
WOS记录号WOS:A1997XU77200011
PM记录号20229304
BIOSIS记录号BCI:BCI199800071025
IR记录号BCI:BCI199800071025
第一机构
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文献类型期刊论文
条目标识符http://ir.lzu.edu.cn/handle/262010/116008
Collection生命科学学院
Recommended Citation:
GB/T 7714
Wang, R,Ni, JM,Hu, XY,et al. Synthesis and DNA-binding ability of Sp1 protein zinc finger domain and its peptidomimetics[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,1997,40(5):518-523.
APA Wang, R.,Ni, JM.,Hu, XY.,Ma, YP.,Li, XQ.,...&Pan, XF.(1997).Synthesis and DNA-binding ability of Sp1 protein zinc finger domain and its peptidomimetics.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,40(5),518-523.
MLA Wang, R,et al."Synthesis and DNA-binding ability of Sp1 protein zinc finger domain and its peptidomimetics".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 40.5(1997):518-523.
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