兰州大学机构库 >基础医学院
RNF168 forms a functional complex with RAD6 during the DNA damage response
Liu, C; Wang, DG; Wu, JX; Keller, J; Ma, T; Yu, XC; Yu, XC (reprint author), Univ Michigan, Sch Med, Dept Internal Med, Div Mol Med & Genet, Ann Arbor, MI 48109 USA.
Indexed BySCIE
2013-05-01
Source PublicationJOURNAL OF CELL SCIENCE
Volume126Issue:9Pages:2042-2051
PublisherCOMPANY OF BIOLOGISTS LTD
Publication PlaceCAMBRIDGE
AbstractProtein ubiquitination plays an important role in initiating the DNA damage response. Following DNA damage, E2 ubiquitin conjugating enzymes are crucial for catalyzing substrate ubiquitination that recruits downstream DNA repair factors to DNA lesions. To identify novel E2 conjugating enzymes important for initiating the DNA-damage-induced ubiquitination cascade, we screened most of the known E2 enzymes and found that RAD6A and RAD6B function together with RNF168 in the ionizing radiation (IR)-induced DNA damage response. Similarly to RNF168-deficient cells, RAD6A- or RAD6B-deficient cells exhibit a reduction in DNA-damage-induced protein ubiquitination. Correspondingly, DNA-damage-induced foci formation of DNA damage repair proteins, such as BRCA1 and 53BP1, is impaired in the absence of RAD6A or RAD6B. Moreover, the RNF168-RAD6 complex targeted histone H1.2 for ubiquitination in vitro and regulated DNA-damage-induced histone H1.2 ubiquitination in vivo. Collectively, these data demonstrate that RNF168, in complex with RAD6A or RAD6B, is activated in the DNA-damage-induced protein ubiquitination cascade.
KeywordRAD6 RNF168 Ubiquitination DNA damage response
Department[Liu, Chao] Fudan Univ, Inst Genet, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200433, Peoples R China;
[Liu, Chao;
Wang, Degui;
Wu, Jiaxue;
Keller, Jennifer;
Ma, Teng;
Yu, Xiaochun] Univ Michigan, Sch Med, Dept Internal Med, Div Mol Med & Genet, Ann Arbor, MI 48109 USA;
[Wang, Degui] Lanzhou Univ, Sch Basic Med Sci, Dept Anat & Histol, Lanzhou 730000, Peoples R China
Subject AreaCell Biology
Project NumberNational Institutes of Health [CA132755, CA130899] ; National Natural Science Foundation of China [81171954] ; Department of Defense
Funding Project国家自然科学基金项目 ; 美国国立卫生研究院项目
Funding OrganizationNSFC ; NIH
DOI10.1242/jcs.122945
ISSN0021-9533
SubtypeArticle
Language英语
WOS IDWOS:000319561700015
First Inst
Citation statistics
Document Type期刊论文
Identifierhttp://ir.lzu.edu.cn/handle/262010/121626
Collection基础医学院
Corresponding AuthorYu, XC (reprint author), Univ Michigan, Sch Med, Dept Internal Med, Div Mol Med & Genet, Ann Arbor, MI 48109 USA.
Recommended Citation
GB/T 7714
Liu, C,Wang, DG,Wu, JX,et al. RNF168 forms a functional complex with RAD6 during the DNA damage response[J]. JOURNAL OF CELL SCIENCE,2013,126(9):2042-2051.
APA Liu, C.,Wang, DG.,Wu, JX.,Keller, J.,Ma, T.,...&Yu, XC .(2013).RNF168 forms a functional complex with RAD6 during the DNA damage response.JOURNAL OF CELL SCIENCE,126(9),2042-2051.
MLA Liu, C,et al."RNF168 forms a functional complex with RAD6 during the DNA damage response".JOURNAL OF CELL SCIENCE 126.9(2013):2042-2051.
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