兰州大学机构库 >基础医学院
Matrine Reduces Proliferation of Human Lung Cancer Cells by Inducing Apoptosis and Changing miRNA Expression Profiles
Liu, YQ; Li, Y; Qin, J; Wang, Q; She, YL; Luo, YL; He, JX; Li, JY; Xie, XD; Xie, XD (reprint author), Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Peoples R China.
Indexed BySCIE ; PubMed ; MEDLINE
2014
Source PublicationASIAN PACIFIC JOURNAL OF CANCER PREVENTION
ISSN1513-7368
Volume15Issue:5Pages:2169-2177
AbstractMatrine, a main active component extracted from dry roots of Sophora flavecens, has been reported to exert antitumor effects on A549 human non-small lung cancer cells, but its mechanisms of action remain unclear. To determine effects of matrine on proliferation of A549 cells and assess possible mechanisms, MTT assays were employed to detect cytotoxicity, along with o flow cytometric analysis of DNA content of nuclei of cells following staining with propidium iodide to analyze cell cycle distribution. Western blotting was performed to determined expression levels of Bax, Bcl-2, VEGF and HDAC1, while a microarray was used to assessed changes of miRNA profiles. In the MTT assay, matrine suppressed growth of human lung cancer cell A549 in a dose- and time-dependent manner at doses of 0.25-2.5 mg/ml for 24h, 48h or 72h. Matrine induced cell cycle arrest in G0/G1 phase and decreased the G2/M phase, while down-regulating the expression of Bcl2 protein, leading to a reduction in the Bcl-2/Bax ratio. In addition, matrine down regulated the expression level of VEGF and HDAC1 of A549 cells. Microarray analysis demonstrated that matrine altered the expression level of miRNAs compared with untreated control A549 cells. In conclusion, matrine could inhibit proliferation of A549 cells, providing useful information for understanding anticancer mechanisms.
KeywordMatrine A549 lung cancer cells miRNA profile apoptosis proliferation
Subject AreaOncology
PublisherASIAN PACIFIC ORGANIZATION CANCER PREVENTION
Publication PlaceGYEONGGI-DO
DOI10.7314/APJCP.2014.15.5.2169
First Inst
Language英语
Project NumberNational Natural Science Foundation of China [81060351/H2810, 81272454]
Funding Project国家自然科学基金项目
Funding OrganizationNSFC
SubtypeArticle
WOS IDWOS:000335651200046
PMID 24716952
Department
[Liu, Yong-Qi;
Li, Yi;
Xie, Xiao-Dong] Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Peoples R China;
[Li, Yi;
Qin, Jie;
Wang, Qian;
She, Ya-Li;
Luo, Ya-Li;
He, Jian-Xin;
Li, Jing-Ya] Gansu Tradit Chinese Med Univ, Inst Syst Biol & TCM Transformat, Lanzhou, Peoples R China...更多
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.lzu.edu.cn/handle/262010/121694
Collection基础医学院
Corresponding AuthorXie, XD (reprint author), Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Peoples R China.
Recommended Citation
GB/T 7714
Liu, YQ,Li, Y,Qin, J,et al. Matrine Reduces Proliferation of Human Lung Cancer Cells by Inducing Apoptosis and Changing miRNA Expression Profiles[J]. ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,2014,15(5):2169-2177.
APA Liu, YQ.,Li, Y.,Qin, J.,Wang, Q.,She, YL.,...&Xie, XD .(2014).Matrine Reduces Proliferation of Human Lung Cancer Cells by Inducing Apoptosis and Changing miRNA Expression Profiles.ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,15(5),2169-2177.
MLA Liu, YQ,et al."Matrine Reduces Proliferation of Human Lung Cancer Cells by Inducing Apoptosis and Changing miRNA Expression Profiles".ASIAN PACIFIC JOURNAL OF CANCER PREVENTION 15.5(2014):2169-2177.
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