兰州大学机构库 >基础医学院
Expression and Underlying Roles of IGFBP-3 in Paclitaxel-Treated Gastric Cancer Sgc-7901 Cells
Huang, G; Dang, ZF; Dang, YM; Cai, W; Li, Y; Chen, YR; Xie, XD; Xie, XD (reprint author), Lanzhou Univ, Sch Basic Med Sci, Lanzhou 730000, Peoples R China.
Indexed BySCIE
2014-08-04
Source PublicationASIAN PACIFIC JOURNAL OF CANCER PREVENTION
ISSN1513-7368
Volume15Issue:14Pages:5741-5745
AbstractPurpose: To study the expression of insulin-like growth factor binding proteins (IGFBPs) in paclitaxel-treated gastric cancer SGC-7901 cells, and to further investigate underlying mechanisms. Materials and Methods: Real time PCR and Western blot assays were applied to detect the mRNA and protein expression of IGFBP-2, -3 and -5 after paclitaxel (10 nM) treatment of SGC-7901 cells. In addition IGFBP-3 expression was silenced by RNA interference to determine effects. Cell viability was determined by MTT assay. Cell cycling and apoptosis were assessed by flow cytometry. Results: Compared to the control group, only IGFBP-3 expression was elevated significantly after paclitaxel (10 nM) treatment (p < 0.05). Paclitaxel treatment caused cell cycle arrest and apoptosis via downregulating Bcl-2 expression. However, the effect could be abrogated by IGFBP-3 silencing. Conclusions: IGFBP-3 exhibits anti-apoptotic effects on paclitaxel-treated SGC-7901 cells via elevating Bcl-2 expression.
KeywordIGFBPs paclitaxel gastric cancer apoptosis
Subject AreaOncology
PublisherASIAN PACIFIC ORGANIZATION CANCER PREVENTION
Publication PlaceGYEONGGI-DO
DOI10.7314/APJCP.2014.15.14.5741
First Inst
Language英语
Project NumberNational Natural Science Foundation of China [81272454]
Funding Project国家自然科学基金项目
Funding OrganizationNSFC
SubtypeArticle
WOS IDWOS:000343723300041
Department[Huang, Gang;
Dang, Ya-Mei;
Cai, Wei;
Li, Yuan;
Chen, Yi-Rong] Lanzhou Univ, Gansu Prov Hosp, Lanzhou 730000, Peoples R China;
[Huang, Gang;
Xie, Xiao-Dong] Lanzhou Univ, Sch Basic Med Sci, Lanzhou 730000, Peoples R China;
[Dang, Zhong-Feng] Lanzhou Univ, Tumor Hosp Gansu Prov, Lanzhou 730000, Peoples R China;
[Chen, Yi-Rong] Lanzhou Univ, Clin Med Coll 1, Lanzhou 730000, Peoples R China
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.lzu.edu.cn/handle/262010/121733
Collection基础医学院
Corresponding AuthorXie, XD (reprint author), Lanzhou Univ, Sch Basic Med Sci, Lanzhou 730000, Peoples R China.
Recommended Citation
GB/T 7714
Huang, G,Dang, ZF,Dang, YM,et al. Expression and Underlying Roles of IGFBP-3 in Paclitaxel-Treated Gastric Cancer Sgc-7901 Cells[J]. ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,2014,15(14):5741-5745.
APA Huang, G.,Dang, ZF.,Dang, YM.,Cai, W.,Li, Y.,...&Xie, XD .(2014).Expression and Underlying Roles of IGFBP-3 in Paclitaxel-Treated Gastric Cancer Sgc-7901 Cells.ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,15(14),5741-5745.
MLA Huang, G,et al."Expression and Underlying Roles of IGFBP-3 in Paclitaxel-Treated Gastric Cancer Sgc-7901 Cells".ASIAN PACIFIC JOURNAL OF CANCER PREVENTION 15.14(2014):5741-5745.
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