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题名: Separation and Determination of Stereoisomeric Impurity of Folinic Acid Diastereomers by CE with Vancomycin as a Selector
作者: Wang, ZY(王兆彦); Mu, CJ; Kang, JW; Hu, ZD(胡之德)
收录类别: SCIE
出版日期: 2012-10
刊名: CHROMATOGRAPHIA
卷号: 75, 期号:19-20, 页码:1211-1215
出版者: SPRINGER
出版地: HEIDELBERG
英文摘要: A highly sensitive and rapid method was developed that involves capillary electrophoresis for separation and determination of the stereoisomeric impurity of folinic acid diastereomers. In this method, vancomycin was used as the chiral selector, and a solution of poly(dimethylacrylamide) (PDMA) was prepared for dynamic coating of the capillary wall to minimize the adsorption of vancomycin. This method was optimized for six factors including concentrations of the organic modifier and vancomycin, pH and concentration of the background electrolyte, column temperature, and separation voltage. The following conditions were established: 100 mM Tris-phosphate buffer (pH 6.0) containing 1.0 mM vancomycin and 5 % acetonitrile at 30 A degrees C, and -15 kV applied voltage on the PDMA dynamically coated capillary. Preliminary validation was performed with the determination of limit of quantification and detection, accuracy, precision, and linearity. Under our optimized method, the folinic acid diastereomers were baseline-separated within 7.5 min, and a (6S,2'S)-calcium folinate sample with 0.08 % stereoisomeric impurity was determined.
关键词: Capillary electrophoresis ; Vancomycin ; PDMA coating ; Folinic acid diastereomers
作者部门: [Wang, Zhaoyan] Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China ; [Wang, Zhaoyan ; Kang, Jingwu] Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China ; [Mu, Changjun] Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China ; [Hu, Zhide] Lanzhou Univ, Sch Chem & Chem Engn, Lanzhou 730000, Peoples R China
通讯作者: Wang, ZY (reprint author), Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China.
学科分类: Biochemistry & Molecular Biology; Chemistry
文章类型: Article
所属项目编号: Fundamental Research Funds for the Central Universities [lzujbky-2010-135, lzujbky-2010-58]
所属项目名称: 中央高校基本科研业务费专项资金
项目资助者: LZU
语种: 英语
DOI: 10.1007/s10337-012-2304-7
ISSN号: 0009-5893
WOS记录号: WOS:000309223800015
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/121933
Appears in Collections:药学院_期刊论文

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Recommended Citation:
Wang, ZY,Mu, CJ,Kang, JW,et al. Separation and Determination of Stereoisomeric Impurity of Folinic Acid Diastereomers by CE with Vancomycin as a Selector[J]. CHROMATOGRAPHIA,2012,75(19-20):1211-1215.
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