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题名: Preparation of Three-Dimensional Vascularized MSC Cell Sheet Constructs for Tissue Regeneration
作者: Ren, LL; Ma, DY; Liu, B(刘斌); Li, JD; Chen, J; Yang, D; Gao, P
收录类别: SCIE ; PubMed ; MEDLINE ; BIOSIS
出版日期: 2014
刊名: BIOMED RESEARCH INTERNATIONAL
页码: -
出版者: HINDAWI
出版地: NEW YORK
英文摘要: Engineering three-dimensional (3D) vascularized constructs remains a challenge due to the inability to form rich microvessel networks. In this study we engineered a prevascularized 3D cell sheet construct for tissue regeneration using human bone marrow-derived mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells as cell sources. hMSCs were cultured to forma thick cell sheet, and human umbilical vein endothelial cells (HUVECs) were then seeded on the hMSCs sheet to form networks. The single prevascularized HUVEC/hMSC cell sheet was folded to forma 3D construct by a modified cell sheet engineering technique. In vitro results indicated that the hMSCs cell sheet promoted the HUVECs cell migration to form networks in horizontal and vertical directions. In vivo results showed that many blood vessels grew into the 3D HUVEC/hMSC cell sheet constructs after implanted in the subcutaneous pocket of immunodeficient mice. The density of blood vessels in the prevascularized constructs was higher than that in the nonprevascularized constructs. Immunohistochemistry staining further showed that in vitro preformed human capillaries in the prevascularized constructs anastomosed with the host vasculature to form functional blood vessels. These results suggest the promising potential of this 3D prevascularized construct using hMSCs cell sheet as a platform for wide applications in engineering vascularized tissues.
作者部门: [Ren, Liling ; Liu, Bin ; Li, Jinda ; Chen, Jia ; Yang, Dan ; Gao, Peng] Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Gansu, Peoples R China ; [Ma, Dongyang] Lanzhou Command PLA, Lanzhou Gen Hosp, Dept Oral & Maxillofacial Surg, Lanzhou 730050, Gansu, Peoples R China
通讯作者: Ren, LL (reprint author), Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Gansu, Peoples R China.
学科分类: Biotechnology & Applied Microbiology; Research & Experimental Medicine
文章类型: Article
所属项目编号: National Natural Science Foundation of China [81170938, 81300860] ; Chinese Fundamental Research Funds for the Central Universities [lzujbky-2013-173]
所属项目名称: 国家自然科学基金项目 ; 中央高校基本科研业务费专项资金
项目资助者: NSFC ; LZU
语种: 英语
DOI: 10.1155/2014/301279
ISSN号: 2314-6133
WOS记录号: WOS:000339315400001
PM记录号: 25110670
BIOSIS记录号: BIOSIS:PREV201400593133
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/122121
Appears in Collections:口腔医学院_期刊论文

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Recommended Citation:
Ren, LL,Ma, DY,Liu, B,et al. Preparation of Three-Dimensional Vascularized MSC Cell Sheet Constructs for Tissue Regeneration[J]. BIOMED RESEARCH INTERNATIONAL,2014:-.
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