兰州大学机构库 >口腔医学院
Preparation of Three-Dimensional Vascularized MSC Cell Sheet Constructs for Tissue Regeneration
Ren, LL(任利玲); Ma, DY; Liu, B(刘斌); Li, JD; Chen, J; Yang, D; Gao, P; Ren, LL (reprint author), Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Gansu, Peoples R China.
收录类别SCIE ; PubMed ; MEDLINE ; BIOSIS
2014
发表期刊BIOMED RESEARCH INTERNATIONAL
页码-
出版者HINDAWI
出版地NEW YORK
摘要Engineering three-dimensional (3D) vascularized constructs remains a challenge due to the inability to form rich microvessel networks. In this study we engineered a prevascularized 3D cell sheet construct for tissue regeneration using human bone marrow-derived mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells as cell sources. hMSCs were cultured to forma thick cell sheet, and human umbilical vein endothelial cells (HUVECs) were then seeded on the hMSCs sheet to form networks. The single prevascularized HUVEC/hMSC cell sheet was folded to forma 3D construct by a modified cell sheet engineering technique. In vitro results indicated that the hMSCs cell sheet promoted the HUVECs cell migration to form networks in horizontal and vertical directions. In vivo results showed that many blood vessels grew into the 3D HUVEC/hMSC cell sheet constructs after implanted in the subcutaneous pocket of immunodeficient mice. The density of blood vessels in the prevascularized constructs was higher than that in the nonprevascularized constructs. Immunohistochemistry staining further showed that in vitro preformed human capillaries in the prevascularized constructs anastomosed with the host vasculature to form functional blood vessels. These results suggest the promising potential of this 3D prevascularized construct using hMSCs cell sheet as a platform for wide applications in engineering vascularized tissues.
作者部门[Ren, Liling;
Liu, Bin;
Li, Jinda;
Chen, Jia;
Yang, Dan;
Gao, Peng] Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Gansu, Peoples R China;
[Ma, Dongyang] Lanzhou Command PLA, Lanzhou Gen Hosp, Dept Oral & Maxillofacial Surg, Lanzhou 730050, Gansu, Peoples R China
学科领域Biotechnology & Applied Microbiology ; Research & Experimental Medicine
所属项目编号National Natural Science Foundation of China [81170938, 81300860] ; Chinese Fundamental Research Funds for the Central Universities [lzujbky-2013-173]
资助项目国家自然科学基金项目 ; 中央高校基本科研业务费专项资金
项目资助者NSFC ; LZU
DOI10.1155/2014/301279
ISSN2314-6133
文章类型Article
语种英语
WOS记录号WOS:000339315400001
PM记录号25110670
BIOSIS记录号BIOSIS:PREV201400593133
第一机构
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文献类型期刊论文
条目标识符http://ir.lzu.edu.cn/handle/262010/122121
Collection口腔医学院
通讯作者Ren, LL (reprint author), Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Gansu, Peoples R China.
Recommended Citation:
GB/T 7714
Ren, LL,Ma, DY,Liu, B,et al. Preparation of Three-Dimensional Vascularized MSC Cell Sheet Constructs for Tissue Regeneration[J]. BIOMED RESEARCH INTERNATIONAL,2014:-.
APA Ren, LL.,Ma, DY.,Liu, B.,Li, JD.,Chen, J.,...&Ren, LL .(2014).Preparation of Three-Dimensional Vascularized MSC Cell Sheet Constructs for Tissue Regeneration.BIOMED RESEARCH INTERNATIONAL,-.
MLA Ren, LL,et al."Preparation of Three-Dimensional Vascularized MSC Cell Sheet Constructs for Tissue Regeneration".BIOMED RESEARCH INTERNATIONAL (2014):-.
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