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题名: Wnt5a Promotes Inflammatory Responses via Nuclear Factor kappa B (NF-kappa B) and Mitogen-activated Protein Kinase (MAPK) Pathways in Human Dental Pulp Cells
作者: Zhao, Y(赵媛); Wang, CL; Li, RM; Hui, TQ; Su, YY; Yuan, Q; Zhou, XD; Ye, L
收录类别: SCIE ; EI
出版日期: 2014-07-25
刊名: JOURNAL OF BIOLOGICAL CHEMISTRY
卷号: 289, 期号:30, 页码:21028-21039
英文摘要: Wnt5a has been found recently to be involved in inflammation regulation through a mechanism that remains unclear. Immunohistochemical staining of infected human dental pulp and tissue from experimental dental pulpitis in rats showed that Wnt5a levels were increased. In vitro, Wnt5a was increased 8-fold in human dental pulp cells (HDPCs) after TNF-alpha stimulation compared with control cells. We then investigated the role of Wnt5a in HDPCs. In the presence of TNF-alpha, Wnt5a further increased the production of cytokines/chemokines, whereas Wnt5a knockdown markedly reduced cytokine/chemokine production induced by TNF-alpha. In addition, in HDPCs, Wnt5a efficiently induced cytokine/chemokine expression and, in particular, expression of IL-8 (14.5-fold) and CCL2 (25.5-fold), as assessed by a Luminex assay. The cytokine subsets regulated by Wnt5a overlap partially with those induced by TNF-alpha. However, no TNF-alpha and IL-1 beta was detected after Wnt5a treatment. We then found that Wnt5a alone and the supernatants of Wnt5a-treated HDPCs significantly increased macrophage migration, which supports a role for Wnt5a in macrophage recruitment and as an inflammatory mediator in human dental pulp inflammation. Finally, Wnt5a participates in dental pulp inflammation in a MAPK-dependent (p38-, JNK-, and ERK-dependent) and NF-kappa B-dependent manner. Our data suggest that Wnt5a, as an inflammatory mediator that drives the integration of cytokines and chemokines, acts downstream of TNF-alpha.
作者部门: [Zhao, Yuan ; Wang, Chen-Lin ; Li, Rui-Min ; Hui, Tian-Qian ; Yuan, Quan ; Zhou, Xue-Dong ; Ye, Ling] Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis, Chengdu 610041, Peoples R China ; [Zhao, Yuan] Lanzhou Univ, Stomatol Coll, Dept Oral Basic Sci, Lanzhou 730000, Peoples R China ; [Su, Ying-Ying] Capital Med Univ, Sch Stomatol, Beijing Key Lab Tooth Regenerat & Funct Reconstru, Mol Lab Gene Therapy & Tooth Regenerat, Beijing 100050, Peoples R China
通讯作者: Ye, L (reprint author), 17 Renmin Nan Lu, Chengdu 610041, Peoples R China.
学科分类: Biochemistry & Molecular Biology
文章类型: Article
所属项目编号: National Nature Science Foundation of China [81070801, 813220170] ; Science and Technology Planning Project of Sichuan Province [2012SZ0034] ; Innovative Research Team of Education Department of Sichuan Province [13TD0038] ; Program of International ST Cooperation [2014DFA31990]
所属项目名称: 国家自然科学基金项目 ; 国家国际科技合作专项
项目资助者: NSFC ; MOST
语种: 英语
DOI: 10.1074/jbc.M113.546523
ISSN号: 0021-9258
WOS记录号: WOS:000339396600055
EI记录号: 20143218044770
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/122122
Appears in Collections:口腔医学院_期刊论文

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