兰州大学机构库 >第二临床医学院
ComCED signal loop precisely regulates nlmC expression in Streptococcus mutans
Liu, TL; Xue, SB; Cai, WB; Liu, XJ; Liu, XJ; Zheng, RR; Luo, HY; Qi, WH; Liu, TL (reprint author), Jiangsu Univ, Sch Food & Bioengn, 508,301 Rd Xuefu, Zhenjiang 212013, Jiangsu, Peoples R China.
2014-03
Source PublicationANNALS OF MICROBIOLOGY
ISSN1590-4261
Volume64Issue:1Pages:31-38
AbstractThe ComED two-component signal transduction system (TCSTS) and the exogenous CSP can provoke the competence development and mutacin production of Streptococcus mutans. More binding fashions of ComE with DNA are the molecular basis for the ComED TCSTS to play pleiotropic roles. The interaction of nlmC and comC in regulation makes the expression of nlmC different from that of nlmAB and the ComE molecular feature is shown in the regulation pattern of nlmC. By electrophoretic mobility shift assay, ComE binding sites were discoveried in PnlmC314 and PnlmC419 for the first time. In the wild-type background, the promoter activity of nlmC is not only stronger than that of nlmAB but also rushes to a peak value of 3,116.0 MU at the point of 100 min in liquid culture with no supply of exogenous CSP; the data indicate that the regulation of nlmC expression is charged in an known coordination mechanism. In the mutant background, by monitoring the promoter activity of nlmC and nlmAB, it is proved that they are both controlled directly by the ComED TCSTS. But the surprising result is the deletion of comC to make nlmC promoter lose its activity completely. Based on those data, it is deduced that Pnlm419, PnlmC314 and PnlmC, which comprise the nlmC regulating region, coordinate to incite nlmC expression and to make nlmC expression different from that of nlmAB because of a synergistic effect, whereas they also could inhibit comC expression by blocking the RNA polymerase moving through the binding of ComE with the comC encoding region.
KeywordStreptococcus mutans Mutacins Electrophoretic mobility shift assay Beta-Galactosidase
Subject AreaBiotechnology & Applied Microbiology ; Microbiology
PublisherSPRINGER
DOI10.1007/s13213-013-0629-6
Publication PlaceNEW YORK
Indexed BySCIE
Language英语
First Inst
Funding Project教育部留学回国人员科研启动基金
Project NumberScientific Research Startup Fund for Advanced Talents of Jiangsu University [10JDG040] ; Priority Academic Program Development of Jiangsu Higher Education Institutions ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
WOS IDWOS:000331648300004
Funding OrganizationMOE
SubtypeArticle
Department[Liu, Tianlei;
Cai, Wenbo;
Liu, Xiaojing;
Liu, Xiaojuan;
Zheng, Rongrong;
Luo, Hongyan;
Qi, Wenhui] Jiangsu Univ, Sch Food & Bioengn, Zhenjiang 212013, Jiangsu, Peoples R China;
[Xue, Shoubin] Lanzhou Univ, Clin Med Coll 2, Lanzhou 730000, Gansu, Peoples R China
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.lzu.edu.cn/handle/262010/126192
Collection第二临床医学院
Corresponding AuthorLiu, TL (reprint author), Jiangsu Univ, Sch Food & Bioengn, 508,301 Rd Xuefu, Zhenjiang 212013, Jiangsu, Peoples R China.
Recommended Citation
GB/T 7714
Liu, TL,Xue, SB,Cai, WB,et al. ComCED signal loop precisely regulates nlmC expression in Streptococcus mutans[J]. ANNALS OF MICROBIOLOGY,2014,64(1):31-38.
APA Liu, TL.,Xue, SB.,Cai, WB.,Liu, XJ.,Liu, XJ.,...&Liu, TL .(2014).ComCED signal loop precisely regulates nlmC expression in Streptococcus mutans.ANNALS OF MICROBIOLOGY,64(1),31-38.
MLA Liu, TL,et al."ComCED signal loop precisely regulates nlmC expression in Streptococcus mutans".ANNALS OF MICROBIOLOGY 64.1(2014):31-38.
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