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题名: Preparation of amine-functionalized mesoporous magnetic colloidal nanocrystal clusters for glucoamylase immobilization
作者: Wang, JZ; Zhao, GH; Li, YF(李彦锋); Peng, XM; Wang, XY
收录类别: SCIE ; EI ; BIOSIS
出版日期: 2015-03-01
刊名: Chemical Engineering Journal
卷号: 263, 页码:471-478
出版者: ELSEVIER
出版地: LAUSANNE
英文摘要: A facile one-pot synthesis of size-tunable mesoporous carboxyl-functionalized magnetic colloidal nanocrystal clusters (MCNCs) with high magnetization (82.0 emu/g), large surface area (95 m(2)/g), and excellent colloidal stability has been developed. The mesostructured MCNCs were synthesized by a solvothermal approach with iron (III) chloride hexahydrate as a precursor, ethylene glycol as a reducing agent, ammonium acetate as a porogen, and ethylenediaminetetraacetic acid disodium salt (EDTA-2Na) as a surface-modification agent. Glucoamylase was immobilized onto the mesoporous MCNCs via the different routes. These immobilized glucoamylase exhibited excellent thermal stability and reusability in comparison with the free enzyme. The residual activity of immobilized enzyme remained above 65% after 6 h, while free glucoamylase was only left over 45% of the initial activity. And the residual activity of the immobilized enzyme was about 60% of the initial activity after the 10th reuse. (C) 2014 Published by Elsevier B.V.
关键词: Mesoporosity ; Magnetic colloidal nanocrystal clusters ; Immobilisation ; Glucoamylase ; Biocatalysis ; Enzyme activity
作者部门: [Wang, Jianzhi ; Zhao, Guanghui ; Li, Yanfeng ; Peng, Xiaomeng ; Wang, Xinyu] Lanzhou Univ, Coll Chem & Chem Engn, Inst Biochem Engn &Environm Technol, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China
通讯作者: Li, YF (reprint author), Lanzhou Univ, Coll Chem & Chem Engn, Inst Biochem Engn &Environm Technol, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China.
学科分类: Engineering
文章类型: Article
所属项目编号: National Natural Science Foundation of China [21374045, 21074049] ; Scientific Research Ability Training of under-graduate students majoring in chemistry by the two patterns based on the tutorial system and top students [11103307] ; Opening Foundation of State Key Laboratory of Applied Organic Chemistry [SKLAOC-2009-35]
所属项目名称: 国家自然科学基金项目 ; 国家基础科学人才培养基金项目
项目资助者: NSFC
语种: 英语
DOI: 10.1016/j.cej.2014.08.007
ISSN号: 1385-8947
WOS记录号: WOS:000349062900053
EI记录号: 20150200404238
BIOSIS记录号: BCI:BCI201500223282
IR记录号: BCI:BCI201500223282
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/126413
Appears in Collections:化学化工学院_期刊论文

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Recommended Citation:
Wang, JZ,Zhao, GH,Li, YF,et al. Preparation of amine-functionalized mesoporous magnetic colloidal nanocrystal clusters for glucoamylase immobilization[J]. CHEMICAL ENGINEERING JOURNAL,2015,263:471-478.
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