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题名: Facile synthesis of oxidic PEG-modified magnetic polydopamine nanospheres for Candida rugosa lipase immobilization
作者: Hou, C; Zhu, H; Li, YF(李彦锋); Li, YJ; Wang, XY; Zhu, WW; Zhou, RD
收录类别: SCIE ; EI ; PubMed ; MEDLINE ; BIOSIS
出版日期: 2015-02
刊名: Applied Microbiology and Biotechnology
卷号: 99, 期号:3, 页码:1249-1259
出版者: SPRINGER
出版地: NEW YORK
英文摘要: A versatile method for the design of polydopamine-coated magnetic material with a brush-like structure used for Candida Rugosa lipase (CRL) immobilization was reported in this work. First, polydopamine (PDA) was coated on the surface of Fe3O4 nanospheres (Fe3O4 NPs) with a controllable thickness via dip coating process, and CRL can be immobilized on it directly via covalent bonding. Subsequently, PDA-functionalized Fe3O4 NPs were modified with dialdehyde polyethylene glycol (PEG) to obtain the aldehyde groups, and the brush-like structure of the magnetic supports was formed. After being characterized with various methods, it was verified that the prepared magnetic NPs possessed good monodispersity and displayed high saturation magnetization after modification. Meanwhile, the CRL was immobilized on it covalently, and the enzyme activities such as activity, stability, and reusability were investigated. Significantly, the versatility of polydopamine-inspired chemistry combined with the unique biological nature and tunability with dialdehyde PEG could evoke the efficiency of the CRL, making this a promising coating technique for various bio-applications.
关键词: Magnetic nanospheres ; Polydopamine ; Enzyme ; Flexible immobilization
作者部门: [Hou, Chen ; Zhu, Hao ; Li, Yanfeng ; Li, Yijing ; Wang, Xinyu ; Zhu, Weiwei ; Zhou, Rongde] Lanzhou Univ, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Coll Chem & Chem Engn,Inst Biochem Engn & Environ, Lanzhou 730000, Peoples R China
通讯作者: Li, YF (reprint author), Lanzhou Univ, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Coll Chem & Chem Engn,Inst Biochem Engn & Environ, Lanzhou 730000, Peoples R China.
学科分类: Biotechnology & Applied Microbiology
文章类型: Article
所属项目编号: National Natural Science Foundation of China [21374045, 21074049] ; scientific research ability training of under-graduate students majoring in chemistry by the two patters based on the tutorial system and top students [J1103307] ; Opening Foundation of State Key Laboratory of Applied Organic Chemistry [SKLAOC-2009-35]
所属项目名称: 国家自然科学基金项目 ; 国家基础科学人才培养基金项目
项目资助者: NSFC
语种: 英语
DOI: 10.1007/s00253-014-5990-2
ISSN号: 0175-7598
WOS记录号: WOS:000348771400020
EI记录号: 20150500476420
PM记录号: 25117546
BIOSIS记录号: BCI:BCI201600333361
IR记录号: BCI:BCI201600333361
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/126423
Appears in Collections:化学化工学院_期刊论文

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Recommended Citation:
Hou, C,Zhu, H,Li, YF,et al. Facile synthesis of oxidic PEG-modified magnetic polydopamine nanospheres for Candida rugosa lipase immobilization[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2015,99(3):1249-1259.
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