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兰州大学机构库  > 化学化工学院  > 期刊论文
题名: Enhanced shRNA Delivery and ABCG2 Silencing by Charge-Reversible Layered Nanocarriers
作者: Chen, ZZ; Zhang, LF; He, YL; Shen, YQ; Li, YF(李彦锋)
收录类别: SCIE ; EI ; PubMed ; MEDLINE
出版日期: 2015-02-25
刊名: SMALL
卷号: 11, 期号:8, 页码:952-962
出版者: WILEY
出版地: WEINHEIM
英文摘要: Polycationic vectors have been used to deliver short hairpin RNAs (shRNAs) to knock-down genes for cancer therapies, but their inefficiency in lysosomal escape and shRNA release causes their low gene transcription efficiency. Herein, a three-layered polyethyleneimine (PEI)-coated gold nanocomplex interlaid with a pH-responsive charge-reversible chitosan-aconitic anhydride (CS-Aco) is constructed: a Au-PEI/ CS-Aco/PEI/shRNA nanoparticle. The negatively charged CS-Aco hydrolyzes into positively charged CS in lysosomes, causing the nanocomposite to disassemble. The released Au-PEI nanoparticles efficiently rupture the lysosomes and thus release the PEI/shRNA polyplexes into cytoplasm, where they quickly disassociate because the PEI chains are short (1.2 kDa). As a consequence, the nanocomplexes display higher shRNA delivery efficiency than the 25 kDa PEI, and efficiently deliver shABCG2 to tumors and markedly silence ABCG2 expression, which sensitizes HepG2 cells to the drugs with minimal toxicity.
作者部门: [Chen, Zhenzhen ; Zhang, Lifen ; He, Yuling ; Li, Yanfeng] Lanzhou Univ, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Coll Chem & Chem Engn, Lanzhou 730000, Peoples R China ; [Shen, Youqing] Zhejiang Univ, Key Lab Biomass Chem Engn, Minist Educ, Ctr Bionanoengn, Hangzhou 310027, Zhejiang, Peoples R China ; [Shen, Youqing] Zhejiang Univ, Dept Chem & Biol Engn, Hangzhou 310027, Zhejiang, Peoples R China
通讯作者: Zhang, LF (reprint author), Lanzhou Univ, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Coll Chem & Chem Engn, Lanzhou 730000, Peoples R China.
学科分类: Chemistry; Science & Technology - Other Topics; Materials Science; Physics
文章类型: Article
所属项目编号: National Natural Science Foundation of China [21104029, 21074049] ; Gansu Province Science Foundation for Youth [1107RJYA038] ; Fundamental Research Funds for the Central Universities [lzujbky-2013-68]
所属项目名称: 国家自然科学基金项目 ; 中央高校基本科研业务费专项资金 ; 甘肃省青年科技基金计划
项目资助者: NSFC ; LZU ; GSSTD
语种: 英语
DOI: 10.1002/smll.201401397
ISSN号: 1613-6810
WOS记录号: WOS:000349978800009
EI记录号: 20150900570422
PM记录号: 25330768
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/126516
Appears in Collections:化学化工学院_期刊论文

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Recommended Citation:
Chen, ZZ,Zhang, LF,He, YL,et al. Enhanced shRNA Delivery and ABCG2 Silencing by Charge-Reversible Layered Nanocarriers[J]. SMALL,2015,11(8):952-962.
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