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题名: Effect of PM2.5 on the level of nuclear factor erythroid-2 related factor 2 in chronic obstructive pulmonary disease mice and its relationship with oxidative stress
其他题名: 细颗粒物对慢性阻塞性肺疾病小鼠肺组织Nrf2水平的影响及与氧化应激的关系
作者: Zhao, Q J; Liu, X J; Zeng, X L; Bao, H R
收录类别: PubMed ; MEDLINE ; CSCD
出版日期: 2016-07-26
刊名: 中华医学杂志
卷号: 96, 期号:28, 页码:2241-2245
期刊主办单位: 中华医学会
出版地: BEIJING
中文摘要: 目的探讨细颗粒物(PM2.5)对慢性阻塞性肺疾病(慢阻肺)小鼠肺组织核因子相关因子2(Nrf2)水平的影响及与氧化应激的关系。方法将40只BALB/c小鼠按随机数字表法随机分为正常对照组、正常PM2.5组、慢阻肺对照组和慢阻肺PM2.5组各10只。慢阻肺模型采用单纯香烟烟雾暴露法建立;PM2.5组小鼠气管内一次性滴入PM2.5混悬液(20 mg/kg)。造模结束后即采用小鼠无创体积描记箱检测正常对照组和慢阻肺对照组小鼠肺功能,并行小鼠肺组织病理学检查,实时荧光定量PCR和Western印迹法检测小鼠肺组织中Nrf2 mRNA和蛋白表达,分别用菲罗啉比色法、改良Hafeman法和硫代巴比妥酸比色法检测肺组织匀浆中总抗氧化能力(TAC)、谷胱甘肽过氧化物酶(GSH-PX)和丙二醛(MDA)含量。结果正常对照组、慢阻肺对照组、正常PM2.5组、慢阻肺PM2.5组肺组织中Nrf2 mRNA和蛋白相对表达量分别为:1.00、4.46±0.42、4.93 ±0. 63、6.41 ±0.35和0.92±0. 08、1.23 ± 0.07、1.20 ±0.09、1.43 ±0.10;慢阻肺对照组均显著高于正常对照组,且正常PM2.5组及慢阻肺PM2.5组均分别显著高于其对照组,慢阻肺PM2.5组显著高于正常PM2.5组(均P<0. 01);各组肺组织匀浆中TAC和GSH-PX分别为:(5.1 ± 0. 4)、(2.9 ± 0.4)、(3.3 ±0.3)、(1.8 ±0.3)和(13.4 ±0. 5)、(9.9 ±0.7)、(9.8 ±0.7)、(7.0 ±0. 6)U/mgpro;慢阻肺对照组均显著低于正常对照组,且正常PM2.5组及慢阻肺PM2.5组均分别显著低于其对照组,慢阻肺PM2.5组显著低于正常PM2.5组(均P <0.01);各组肺组织匀浆中MDA分别为:(2.9 ±0.4)、(4.8 ±0.5)、(4.5 ± 0.3)、(6.2 ±0. 4)nmol/mgpro;慢阻肺对照组显著高于正常对照组,且正常PM2.5组及慢阻肺PM2.5组均分别显著高于其对照组,慢阻肺PM2.5组显著高于正常PM2.5组(均P < 0.01)。各组小鼠Nrf2 mRNA、蛋白表达均与TAC、GSH-PX呈负相关,与MDA呈正相关。结论PM2.5能够刺激慢阻肺肺组织中Nrf2的表达,加剧肺组织的氧化应激,Nrf2的高表达与氧化应激状态恶化有关。
英文摘要: OBJECTIVE: To explore the effects of fine particulate matter on the level of nuclear factor erythroid-2 related factor 2 (Nrf2) in pulmonary tissues of chronic obstructive pulmonary disease (COPD) mouse models and its relationship with oxidative stress.METHODS: Totally 40 BALB/c mice were randomly divided into normal control group, normal PM2.5 group, COPD control group and COPD PM2.5 group.COPD mice were established using exposure of cigarette smoking.PM2.5 (20 mg/kg) was intratracheally instilled in PM2.5 group mice.Mice pulmonary function was measured by mice noninvasive body plethysmograph and lung histopathology was observed in normal control group and normal PM2.5 group mice.The mRNA and protein expression of Nrf2 was measured with real-time polymerase chain reaction (PCR) and Western blot methods.Total antioxidative capacity (TAC) was measured by O-phenanthroline colorimetry.Glutathione peroxidase (GSH-PX) was measured by improved Hafeman colorimetry and malondialdehyde (MDA) by thiobarbiturieacid colorimetry.RESULTS: Nrf2 mRNA and protein in normal control group, normal PM2.5 group, COPD control group and COPD PM2.5 group were 1.00, 4.46±0.42, 4.93±0.63, 6.41±0.35 and 0.92±0.08, 1.23±0.07, 1.20±0.09, 1.43±0.10.Nrf2 mRNA and protein in COPD control group were increased than those in normal control group while those in normal PM2.5 group and COPD PM2.5 group were respectively higher than each control group.Comparing to normal PM2.5 group, the Nrf2 mRNA and protein in COPD PM2.5 group were increased (all P<0.01). TAC and GSH-PX in each group were (5.1±0.4), (2.9±0.4), (3.3±0.3), (1.8±0.3) and (13.4±0.5), (9.9±0.7), (9.8±0.7), (7.0±0.6) U/mgpro.TAC and GSH-PX in COPD control group were decreased than those in normal control group while those in normal PM2.5 group and COPD PM2.5 group were respectively lower than each control group.Comparing to normal PM2.5 group, the Nrf2 mRNA and protein in COPD PM2.5 group were decreased (all P<0.01). MDA in each group were (2.9±0.4), (4.8±0.5), (4.5±0.3), and (6.2±0.4) nmol/mgpro.MDA in COPD control group were increased than those in normal control group while those in normal PM2.5 group and COPD PM2.5 group were respectively higher than each control group.Comparing to normal PM2.5 group, the MDA in COPD PM2.5 group were increased (all P<0.01). Positive correlations were observed between Nrf2 mRNA, protein and MDA, while negative correlations were observed between Nrf2 mRNA, protein and TAC, GSH-PX in all groups (all P<0.05).CONCLUSIONS: PM2.5 can induce Nrf2 expression and aggravate oxidative stress in COPD mice.The increased expression of Nrf2 is closely associated with oxidative stress.
关键词: 肺疾病 ; 慢性阻塞性 ; 颗粒物 ; 核因子相关因子2 ; 氧化性应激
作者部门: Department of Gerontal Respiratory Medicine, the First Hospital of Lanzhou University, Lanzhou 730000, China.
文章类型: Article
所属项目编号: 甘肃省科技支撑计划 ; 兰州市科技计划项目
所属项目名称: 甘肃省科技支撑计划 ; 兰州市科技局资助项目
项目资助者: GSSTD ; LZSTB
语种: 中文
DOI: 10.3760/cma.j.issn.0376-2491.2016.28.009
ISSN号: 0376-2491
CSCD记录号: CSCD:5761315
PM记录号: 27480657
IR记录号: 27480657
第一机构:
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/178662
Appears in Collections:第一临床医学院_期刊论文

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Recommended Citation:
Zhao, Q J,Liu, X J,Zeng, X L,et al. Effect of PM2.5 on the level of nuclear factor erythroid-2 related factor 2 in chronic obstructive pulmonary disease mice and its relationship with oxidative stress[J]. Zhonghua yi xue za zhi,2016,96(28):2241-2245.
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