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题名: Highly sensitive fluorescence detection of trypsin based on gold nanoparticle probes
作者: Zhao, XR; Chen, YL; Wang, L; Wang, WF; Chen, XG(陈兴国)
收录类别: SCIE ; EI
出版日期: 2016
刊名: ANALYTICAL METHODS
卷号: 8, 期号:2, 页码:393-400
英文摘要: In this study, a simple, sensitive, and selective fluorometric platform has been constructed for measuring trypsin in biological samples using the prepared Au-peptide-FITC beacon. The fluorescence of FITC was quenched by Au NPs owing to the FRET mechanism and recovered after selective cleavage of the beacon peptide by trypsin. The calibration plot for trypsin was achieved over the concentration range of 10-100 nmol L-1 with a correlation coefficient of 0.995 and a limit of detection of 5 nmol L-1. In addition, the application of the present approach in determining the trypsin content in blood plasma samples was demonstrated to be satisfactory, which suggested its potential for diagnostic purpose.
作者部门: [Zhao, Xin-rui ; Chen, Yong-lei ; Wang, Lang ; Wang, Wei-feng ; Chen, Xing-guo] Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China ; [Zhao, Xin-rui ; Chen, Yong-lei ; Wang, Lang ; Wang, Wei-feng ; Chen, Xing-guo] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China ; [Chen, Xing-guo] Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China
通讯作者: Chen, XG (reprint author), Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China.
学科分类: Chemistry; Food Science & Technology; Spectroscopy
文章类型: Article
所属项目编号: National Natural Science Foundation of China [21375053] ; Specialized Research Fund for the Doctorial Program of Higher Education [20130211110039]
所属项目名称: 国家自然科学基金项目 ; 高等学校博士学科点专项科研基金
项目资助者: NSFC ; MOE
语种: 英语
DOI: 10.1039/c5ay02405f
ISSN号: 1759-9660
WOS记录号: WOS:000367243600021
EI记录号: 20160201769539
IR记录号: WOS:000367243600021
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/179932
Appears in Collections:化学化工学院_期刊论文

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Recommended Citation:
Zhao, XR,Chen, YL,Wang, L,et al. Highly sensitive fluorescence detection of trypsin based on gold nanoparticle probes[J]. ANALYTICAL METHODS,2016,8(2):393-400.
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