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题名: A new fluorescence turn-on probe for biothiols based on photoinduced electron transfer and its application in living cells
作者: Wang, JX; Zhou, C; Zhang, JJ; Zhu, XY; Liu, XY; Wang, Q; Zhang, HX(张海霞)
收录类别: SCIE ; EI ; MEDLINE ; BIOSIS
出版日期: 2016-09-05
刊名: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷号: 166, 页码:31-37
英文摘要: A new biothiol-selective fluorescent probe 1 based on photoinduced electron transfer (PET) mechanism was designed and synthesized. The UV-Vis absorption and fluorescent emission properties of probe 1 towards various analytes were studied in detail. The probe exhibited a large stokes shift (similar to 200 nm) after reacted with biothiols and could selectively detect cysteine (Cys) in dimethyl sulfoxide (DMSO)/H2O solution (9:1, v/v, 10 mM phosphate buffer saline, pH 3.5) over glutathione (GSH), homocysteine (Hcy) and other analytes with a detection limit of 0.117 mu M. In addition, probe 1 responded well to GSH, Hcy and Cys in the same above solution with pH 5.5 and got the detection limits of 0.151 mu M, 0.128 mu M and 0.037 mu M, respectively. Probe 1 was of very low cytotoxicity and successfully applied for imaging of thiols in living cells. (C) 2016 Elsevier B.V. All rights reserved.
关键词: Fluorescent probe ; Photoinduced electron transfer ; Imaging ; Biothiols
作者部门: [Wang, Jianxi ; Zhang, Jianjian ; Zhu, Xinyue ; Liu, Xiaoyan ; Zhang, Haixia] Lanzhou Univ, Key Lab Nonferrous Met Chem & Resources Utilizat, State Key Lab Appl Organ Chem, Coll Chem & Chem Engn, Lanzhou 730000, Peoples R China ; [Zhou, Cheng ; Wang, Qin] Lanzhou Univ, Sch Life Sci, Dept Cell Biol, Lanzhou 730000, Peoples R China
通讯作者: Zhang, HX (reprint author), Lanzhou Univ, Key Lab Nonferrous Met Chem & Resources Utilizat, State Key Lab Appl Organ Chem, Coll Chem & Chem Engn, Lanzhou 730000, Peoples R China.
学科分类: Spectroscopy
文章类型: Article
所属项目编号: National Science Foundation of China [21375052, 21575055]
所属项目名称: 国家自然科学基金项目
项目资助者: NSFC
语种: 英语
DOI: 10.1016/j.saa.2016.05.004
ISSN号: 1386-1425
WOS记录号: WOS:000378671600006
EI记录号: 20162102412194
PM记录号: 27203232
BIOSIS记录号: BCI:BCI201600681776
IR记录号: WOS:000378671600006
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/180569
Appears in Collections:化学化工学院_期刊论文

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Recommended Citation:
Wang, JX,Zhou, C,Zhang, JJ,et al. A new fluorescence turn-on probe for biothiols based on photoinduced electron transfer and its application in living cells[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2016,166:31-37.
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