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题名: A colorimetric and fluorescent probe for detecting intracellular biothiols
作者: Chen, CY; Liu, W; Xu, C; Liu, WS(刘伟生)
收录类别: SCIE ; EI ; PubMed ; MEDLINE ; BIOSIS
出版日期: 2016-11-15
刊名: BIOSENSORS & BIOELECTRONICS
卷号: 85, 页码:46-52
出版者: ELSEVIER
出版地: OXFORD
英文摘要: A new rapid and highly sensitive coumarin-based probe (probe 1) has been designed and synthesized for detecting intracellular thiols. Probe 1 was prepared by a 4-step procedure as a latent fluorescence probe to achieve high sensitivity and fluorescence turn-on response toward cysteine and homocysteine over GSH and other various natural amino acids under physiological conditions. Owing to specific cyclization between thiols and aldehyde group, probe 1 displayed a highly selectivity toward cysteine and homocysteine. Above all, probe 1 was successfully used for fluorescence imaging of biothiols in Hela cells, and quantitative determination had been achieved within a certain range. Then specific fluorescence imaging of mice organ tissues was obtained for proving the permeability of probe 1. Simultaneously, the viability was measured to be more than 80%, which shows probe 1 can be a rapid and biocompatible probe for biothiols in cells. Furthermore, the measurement of thiols detection in 5 kinds of animal serum showed that probe 1 can be used in determination of biothiols in blood. (C) 2016 Elsevier B.V. All rights reserved.
关键词: Coumarin ; Fluorescent ; Thiols ; Colorimetric ; Probe ; Bioimaging
作者部门: [Liu, Weisheng] Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China ; Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China
通讯作者: Liu, WS (reprint author), Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China.
学科分类: Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics
文章类型: Article
所属项目编号: National Natural Science Foundation of China [21431002]
所属项目名称: 国家自然科学基金项目
项目资助者: NSFC
语种: 英语
DOI: 10.1016/j.bios.2016.04.098
ISSN号: 0956-5663
WOS记录号: WOS:000382410100006
EI记录号: 20161902351040
PM记录号: 27155115
BIOSIS记录号: BCI:BCI201600759726
IR记录号: WOS:000382410100006
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/180596
Appears in Collections:化学化工学院_期刊论文

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Recommended Citation:
Chen, CY,Liu, W,Xu, C,et al. A colorimetric and fluorescent probe for detecting intracellular biothiols[J]. BIOSENSORS & BIOELECTRONICS,2016,85:46-52.
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