兰州大学机构库 >化学化工学院
A colorimetric and fluorescent probe for detecting intracellular biothiols
Chen, CY; Liu, W; Xu, C; Liu, WS(刘伟生); Liu, WS (reprint author), Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China.
2016-11-15
发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
卷号85页码:46-52
出版者ELSEVIER
摘要A new rapid and highly sensitive coumarin-based probe (probe 1) has been designed and synthesized for detecting intracellular thiols. Probe 1 was prepared by a 4-step procedure as a latent fluorescence probe to achieve high sensitivity and fluorescence turn-on response toward cysteine and homocysteine over GSH and other various natural amino acids under physiological conditions. Owing to specific cyclization between thiols and aldehyde group, probe 1 displayed a highly selectivity toward cysteine and homocysteine. Above all, probe 1 was successfully used for fluorescence imaging of biothiols in Hela cells, and quantitative determination had been achieved within a certain range. Then specific fluorescence imaging of mice organ tissues was obtained for proving the permeability of probe 1. Simultaneously, the viability was measured to be more than 80%, which shows probe 1 can be a rapid and biocompatible probe for biothiols in cells. Furthermore, the measurement of thiols detection in 5 kinds of animal serum showed that probe 1 can be used in determination of biothiols in blood. (C) 2016 Elsevier B.V. All rights reserved.
出版地OXFORD
关键词Coumarin Fluorescent Thiols Colorimetric Probe Bioimaging
学科领域Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
作者部门[Liu, Weisheng] Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China;
Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China
DOI10.1016/j.bios.2016.04.098
文章类型Article
收录类别SCIE ; EI ; PubMed ; MEDLINE ; BIOSIS
所属项目编号National Natural Science Foundation of China [21431002]
语种英语
资助项目国家自然科学基金项目
项目资助者NSFC
WOS记录号WOS:000382410100006
EI记录号20161902351040
PM记录号27155115
BIOSIS记录号BCI:BCI201600759726
IR记录号WOS:000382410100006
第一机构
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文献类型期刊论文
条目标识符http://ir.lzu.edu.cn/handle/262010/180596
Collection化学化工学院
通讯作者Liu, WS (reprint author), Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China.
Recommended Citation:
GB/T 7714
Chen, CY,Liu, W,Xu, C,et al. A colorimetric and fluorescent probe for detecting intracellular biothiols[J]. BIOSENSORS & BIOELECTRONICS,2016,85:46-52.
APA Chen, CY,Liu, W,Xu, C,Liu, WS,&Liu, WS .(2016).A colorimetric and fluorescent probe for detecting intracellular biothiols.BIOSENSORS & BIOELECTRONICS,85,46-52.
MLA Chen, CY,et al."A colorimetric and fluorescent probe for detecting intracellular biothiols".BIOSENSORS & BIOELECTRONICS 85(2016):46-52.
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