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兰州大学机构库  > 化学化工学院  > 期刊论文
题名: A naked-eye visible and turn-on fluorescence probe for Fe3+and its bioimaging application in living cells
作者: Liu, Yaming; Zhang, Jinyu; Ru, Jiaxi; Yao, Xiang; Yang, Yang; Li, Xinghua; Tang, XL(唐晓亮); Zhang, Guolin; Liu, WS(刘伟生)
收录类别: SCIE ; EI
出版日期: 2016-12-01
刊名: SENSORS AND ACTUATORS B-CHEMICAL
卷号: 237, 页码:501-508
出版者: ELSEVIER
出版地: LAUSANNE
英文摘要: A novel turn-on fluorescence probe based on rhodamine B for Fe3+ has been synthesized and characterized by various analytical techniques. Compared with other commonly coexisting metal ions, the probe showed 100-fold fluorescence enhancement for detecting Fe3+ with a 1:1 stoichiometry in MeOH/H2O (1:1, v/v) solution. After adding Fe3+, the rapid enhancement of fluorescence intensity provided a rapid method for detection of Fe3+ The color change was visible, so it could be used for naked-eye detection. The association constant between the probe and Fe3+ was detected to be 0.67 x 10(4) M-1 and the corresponding detection limit were calculated to be 5.7 x 10(-8) M according to fluorescence titration analysis. The reversible binding of probe L to Fe3+ was confirmed by reacting with Na4P2O7, which captured the Fe3+ from the L-Fe3+ complex and turned off the L-Fe(3+)complex. Fluorescence imaging experiments demonstrated that this probe was cell permeable and suitable for monitoring intracellular Fe3+ in living cells by confocal microscopy. What's more, MU assay indicated that this probe had no significant cytotoxicity to living cells. (C) 2016 Elsevier B.V. All rights reserved.
关键词: Naked-eye ; Turn-on ; Fluorescence probe ; Fe3+ ; Bioimaging
作者部门: (1) Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou ; 730000, China ; (2) School of Architecture and Design, Southwest Jiaotong University, Chengdu ; 610031, China
通讯作者: Zhang, GL ; Liu, WS (reprint author), Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China. ; Zhang, GL ; Liu, WS (reprint author), Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China.
学科分类: Chemistry; Electrochemistry; Instruments & Instrumentation
文章类型: Article
所属项目编号: National Natural Scientific Foundation of China (NNSFC) [21431002]
所属项目名称: 国家自然科学基金项目
项目资助者: NSFC
语种: 英语
DOI: 10.1016/j.snb.2016.06.123
ISSN号: 0925-4005
WOS记录号: WOS:000384010500061
EI记录号: 20162802589955
IR记录号: 20162802589955
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/180602
Appears in Collections:化学化工学院_期刊论文

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Recommended Citation:
Liu, Yaming,Zhang, Jinyu,Ru, Jiaxi,et al. A naked-eye visible and turn-on fluorescence probe for Fe3+and its bioimaging application in living cells[J]. Sensors and Actuators, B: Chemical,2016,237:501-508.
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