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Effect of lactic acid bacteria isolated from Tibetan Plateau on silage fermentation quality of Elms nutans
Alternative Title青藏高原乳酸菌对垂穗披碱草青贮饲料发酵品质的影响
Zhang, Hongmei; Ke, Wencan; Jing, Peixin; Zhang, Juan; Chen, Ming; Yu, Yingwen; Guo, Xusheng
Indexed ByPubMed ; MEDLINE ; BIOSIS ; CSCD
2015-10-04
Source Publication微生物学报
ISSN0001-6209
Volume55Issue:10Pages:1291-1297
Abstract[Objective] In order to detect the effect of lactic acid bacteria isolated from Tibetan Plateau on silage fermentation quality of Elms nutans. [Methods] We used 3 isolated lactic acid bacteria with better growth at low temperatures of 10 and 15 degrees C at ensiling of Elymus nutans. Subsequently, effects of the selected lactic acid bacteria on fermentation profiles of Elymus nutans silages stored at 15 and 25 degrees C were evaluated by using the same species of commercial inoculants as the control. [Results] PP-6 isolated from Tibetan Plateau could ferment raffinose, lactose, sorbitol, melibiose and sucrose, and LS-5 could ferment cottonseed sugar, laetrile, rhamnose, lactose, sorbitol, xylose, arabinose, melibiose and sucrose, but the same species of commercial strains could not use these sugars. Inoculation of these three strains into Elymus nutans at 15 and 25 degrees C ensiled for 50 d, we found that LS-5 significantly reduced silage pH, propionic acid concentration and ratio of ammonia nitrogen/total nitrogen at 15 degrees C (P<0.05), salvaged more water-soluble carbohydrate and crude protein; Application of LP-2 and PP-6 as a combined inoculant to Elymus nutans significantly improved lactic acid concentration (P<0.05), resulting in a lower ratio of ammonia nitrogen/total nitrogen, saved more crude protein and significantly reduced neutral detergent fiber content (P<0.05) as compared with the commercial strains. [Conclusion] The three isolated strains can improve silage quality of Elymus nutans growing on the Qinghai-Tibetan Plateau at low temperature, but these strains have no obvious advantages at 25 degrees C in comparison with the commercial inoculants.
Other Abstract【目的】为了探究青藏高原乳酸菌对垂穗披碱草发酵品质的影响。【方法】本试验将3株从青藏高原垂穗披碱草(Elymus nutans Griseb.)中筛选的耐低温乳酸菌(戊糖片球菌PP-6,植物乳杆菌LP-2和清酒乳杆菌LS-5)作为添加剂加入垂穗披碱草进行青贮,同时对其生理生化特性进行研究,并以低海拔条件下分离得到的相同菌种的商品化乳酸菌制剂为对照,研究所筛选的3株优良乳酸菌在低温(15℃)和常温条件(25℃)下对垂穗披碱草青贮饲料发酵品质的影响。【结果】试验结果表明,在碳源利用方面,分离得到的戊糖片球菌PP-6(Pediococcus pentosaceus)发酵棉子糖、乳糖、山梨醇、蜜二糖和蔗糖,清酒乳杆菌LS-5(lactobacillus sakei)可发酵棉子糖、苦杏仁苷、鼠李糖、乳糖、山梨醇、木糖、阿拉伯糖、蜜二糖和蔗糖,而相同菌种的商品菌均不能利用,表明高原乳酸菌具有更广泛的糖源利用特性。将筛选得到的3株低温生长优良菌株进行青藏高原垂穗披碱草青贮发酵试验,青贮50 d后,与对照的商品菌处理相比,在15℃时,清酒乳杆菌LS-5显著降低了青贮饲料p H值、丙酸含量和氨态氮/全氮值(P<0.05),且能够保存更多的水溶性碳水化合物和粗蛋白;植物乳杆菌LP-2(lactobacillus plantarum)和戊糖片球菌PP-6作为复合添加剂显著提高了青贮饲料乳酸含量(P<0.05),显著降低了氨态氮/全氮值和中性洗涤纤维含量(P<0.05),能够保存更多的粗蛋白。但在25℃时,此3株菌与对照菌种相比均无明显优势。【结论】上述结果表明,从青藏高原分离筛选的3株菌种在低温条件下可有效提高青藏高原垂穗披碱草青贮饲料的发酵品质。
Keyword乳酸菌 青贮 低温发酵 垂穗披碱草 青藏高原
Subject AreaBiotechnology & Applied Microbiology ; Environmental Sciences & Ecology
Publication PlaceBeijing
First Inst
Host of Journal中国科学院微生物研究所 ; 中国微生物学会
Language中文
Project Number国家自然科学基金
Funding Project国家自然科学基金项目
Funding OrganizationNSFC
SubtypeArticle
CSCD IDCSCD:5532846
PMID 26939457
BIOSIS IDBCI:BCI201600084189
IRIDBCI:BCI201600084189
DepartmentGuo, Xusheng;
Lanzhou Univ, Sch Life Sci, State Key Lab Grassland and AgroEcosyst, Lanzhou 730000, Gansu Province, Peoples R China
Citation statistics
Cited Times:3[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.lzu.edu.cn/handle/262010/181903
Collection生命科学学院
Recommended Citation
GB/T 7714
Zhang, Hongmei,Ke, Wencan,Jing, Peixin,et al. Effect of lactic acid bacteria isolated from Tibetan Plateau on silage fermentation quality of Elms nutans[J]. 微生物学报,2015,55(10):1291-1297.
APA Zhang, Hongmei.,Ke, Wencan.,Jing, Peixin.,Zhang, Juan.,Chen, Ming.,...&Guo, Xusheng.(2015).Effect of lactic acid bacteria isolated from Tibetan Plateau on silage fermentation quality of Elms nutans.微生物学报,55(10),1291-1297.
MLA Zhang, Hongmei,et al."Effect of lactic acid bacteria isolated from Tibetan Plateau on silage fermentation quality of Elms nutans".微生物学报 55.10(2015):1291-1297.
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