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题名: De Novo Transcriptome Sequencing of Desert Herbaceous Achnatherum splendens (Achnatherum) Seedlings and Identification of Salt Tolerance Genes
作者: Liu, JT; Zhou, YL; Luo, CX; Xiang, Y; An, LZ(安黎哲)
收录类别: SCIE ; MEDLINE ; BIOSIS
出版日期: 2016-04
刊名: GENES
卷号: 7, 期号:4
英文摘要: Achnatherum splendens is an important forage herb in Northwestern China. It has a high tolerance to salinity and is, thus, considered one of the most important constructive plants in saline and alkaline areas of land in Northwest China. However, the mechanisms of salt stress tolerance in A. splendens remain unknown. Next-generation sequencing (NGS) technologies can be used for global gene expression profiling. In this study, we examined sequence and transcript abundance data for the root/leaf transcriptome of A. splendens obtained using an Illumina HiSeq 2500. Over 35 million clean reads were obtained from the leaf and root libraries. All of the RNA sequencing (RNA-seq) reads were assembled de novo into a total of 126,235 unigenes and 36,511 coding DNA sequences (CDS). We further identified 1663 differentially-expressed genes (DEGs) between the salt stress treatment and control. Functional annotation of the DEGs by gene ontology (GO), using Arabidopsis and rice as references, revealed enrichment of salt stress-related GO categories, including oxidation reduction, transcription factor activity, and ion channel transporter. Thus, this global transcriptome analysis of A. splendens has provided an important genetic resource for the study of salt tolerance in this halophyte. The identified sequences and their putative functional data will facilitate future investigations of the tolerance of Achnatherum species to various types of abiotic stress.
关键词: salinity stress ; Achnatherum splendens ; time course ; differentially expressed gene ; expression patterns
作者部门: [Liu, Jiangtao ; Zhou, Yuelong ; Luo, Changxin ; Xiang, Yun ; An, Lizhe] Lanzhou Univ, Sch Life Sci, MOE Key Lab Cell Act & Stress Adaptat, Lanzhou 730000, Peoples R China
通讯作者: An, LZ (reprint author), Lanzhou Univ, Sch Life Sci, MOE Key Lab Cell Act & Stress Adaptat, Lanzhou 730000, Peoples R China.
学科分类: Genetics & Heredity
文章类型: Article
所属项目编号: National Basic Research Program [2014CB954203] ; Fundamental Research Funds for Central Universities [lzujbky-2014-bt05] ; NCBI Sequence Read Archive database (SRA) [SRP068186]
所属项目名称: 国家重点基础研究发展计划以及国家重大科学研究计划(973计划) ; 中央高校基本科研业务费专项资金
项目资助者: MOST ; LZU
语种: 英语
DOI: 10.3390/genes7040012
ISSN号: 2073-4425
WOS记录号: WOS:000375232600005
PM记录号: 27023614
BIOSIS记录号: BCI:BCI201600484789
IR记录号: WOS:000375232600005
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/181991
Appears in Collections:生命科学学院_期刊论文

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Recommended Citation:
Liu, JT,Zhou, YL,Luo, CX,et al. De Novo Transcriptome Sequencing of Desert Herbaceous Achnatherum splendens (Achnatherum) Seedlings and Identification of Salt Tolerance Genes[J]. GENES,2016,7(4).
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