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题名: DNA barcoding of recently diversified tree species: a case study on spruces based on 20 DNA fragments from three different genomes
作者: Zou, JB; Yue, W; Li, LL; Wang, X; Lu, J; Duan, BB; Liu, JQ(刘建全)
收录类别: SCIE ; EI ; BIOSIS
出版日期: 2016-06
刊名: TREES-STRUCTURE AND FUNCTION
卷号: 30, 期号:3, 页码:959-969
英文摘要: A tiered barcoding system comprising traditional cytoplasmic DNAs and multiple nuclear DNAs should be developed to barcode recently diversified tree species. In this study, we aimed to test whether certain nuclear genes are more powerful tools for barcoding spruce species than chloroplast and mitochondrial DNA fragments (DNAs). We sequenced 20 DNAs, including five chloroplast DNAs, two mitochondrial DNAs, and 13 nuclear genes, for a total of 123 individuals representing 37 spruce species and subspecies. We found that some nuclear genes did show higher discriminatory power than any of the chloroplast or mitochondrial DNA fragments we examined. However, no single DNA fragment could discriminate more than half of the spruce species, although a combination of all the nuclear genes greatly increased discrimination success (up to more than 89 % of the total number of species). These findings suggested that most spruce species are derived from recent diversification and still on their way to the final stage of speciation. A low level of accumulation of genetic variation along each species lineage renders it impossible to develop any single nuclear DNA fragment as a barcode. However, combining variations from multiple nuclear genes makes it possible to discriminate between most spruce species. A tiered barcoding system is therefore needed for spruces, and the second tier should comprise multiple nuclear DNAs developed specifically for this genus. These findings provide a case study on discriminating and barcoding recently diverged species, especially for those tree species with large effective population sizes and long life histories.
关键词: Spruce ; DNA barcoding ; Chloroplast DNA ; Mitochondrial DNA ; Nuclear genes
作者部门: [Zou, Jiabin ; Yue, Wei ; Li, Lili ; Wang, Xi ; Lu, Jing ; Duan, Bingbing ; Liu, Jianquan] Lanzhou Univ, Sch Life Sci, State Key Lab Grassland Agroecosyst, Lanzhou 730000, Gansu, Peoples R China
通讯作者: Liu, JQ (reprint author), Lanzhou Univ, Sch Life Sci, State Key Lab Grassland Agroecosyst, Lanzhou 730000, Gansu, Peoples R China.
学科分类: Forestry
文章类型: Article
所属项目编号: National Key Project for Basic Research [2014CB954100] ; National Natural Science Foundation of China [31590821] ; Ministry of Science and Technology of China [2010DFA34610]
所属项目名称: 国家自然科学基金项目 ; 国家重点基础研究发展计划以及国家重大科学研究计划(973计划) ; 国家国际科技合作专项
项目资助者: NSFC ; MOST
语种: 英语
DOI: 10.1007/s00468-015-1337-6
ISSN号: 0931-1890
WOS记录号: WOS:000376281100032
EI记录号: 20155001672184
BIOSIS记录号: BCI:BCI201600580540
IR记录号: WOS:000376281100032
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/182020
Appears in Collections:生命科学学院_期刊论文

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Recommended Citation:
Zou, JB,Yue, W,Li, LL,et al. DNA barcoding of recently diversified tree species: a case study on spruces based on 20 DNA fragments from three different genomes[J]. TREES-STRUCTURE AND FUNCTION,2016,30(3):959-969.
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