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题名: Study on the apoptosis and Bcl-2/Bax expression of human hepatoma SMMC-7721 cells after exposure to heavy-ion and X-ray irradiations
作者: Ye, LP; Hua, X; Jin, XD; Qiang, L; An, LZ(安黎哲)
收录类别: SCIE ; CPCI-S
出版日期: 2007-11
会议名称: 1st International Conference on Applications of Radiotracers in Chemical Environmental and Biological Sciences
会议日期: JAN 23-27, 2006
会议地点: Kolkata, INDIA
英文摘要: This study is aimed at observing the apoptosis and Bcl-2/Bax gene expression of mammalian cells following heavy-ion and X-ray irradiations. Exponentially growing human hepatoma SMMC-7721 cells cultured in vitro were irradiated with a C-12 ion beam of 50 MeV/u (corresponding to a LET value of 44.56 keV/mu m) from Heavy Ion Research Facility in Lanzhou (HIRFL) at doses varying from 0 to 3 Gy. The X-ray irradiation (8 MV) was performed in the therapy unit of the General Hospital of the Lanzhou Military Area. Survival fractions of irradiated cells at various doses were measured by means of MTT assay. Apoptotic cells after irradiation were analyzed with fluorescence microscope and flow cytometer (FCM). Immuno-histological assay were applied to detect the expression of Bcl-2/Bax genes in the irradiated cells. The survival fraction of SMMC-7721 cells decreased gradually (vs. control p<0.05) with increasing the dose of the carbon ion beam more obviously than X-ray irradiation, and the carbon ion irradiation efficiently induced cell apoptosis and significantly promoted the expression of Bax gene while Bcl-2 gene expression was restrained. High-LET heavy ion beam would induce cell apoptosis effectively than low-LET X-ray, and the apoptosis rate is correlated with the transcription of Bcl-2/Bax and the ratio of Bcl-2/Bax in human hepatoma SMMC-7721 cells after irradiation to heavy ion beam.
作者部门: Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China ; Lanzhou Univ, Inst Modern Phys, Lanzhou 730000, Peoples R China
通讯作者: Ye, LP (reprint author), Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China.
学科分类: Chemistry; Nuclear Science & Technology
会议主办者: Saha Inst Nucl Phys
会议录: JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
卷号: 274
期号: 2
页码: 429-434
出版者: SPRINGER
出版地: DORDRECHT
语种: 英语
DOI: 10.1007/s10967-007-1132-y
ISSN号: 0236-5731
WOS记录号: WOS:000250670700033
IR记录号: WOS:000250670700033
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内容类型: 会议论文
URI标识: http://ir.lzu.edu.cn/handle/262010/184767
Appears in Collections:生命科学学院_会议论文

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Recommended Citation:
Ye, LP,Hua, X,Jin, XD,et al. Study on the apoptosis and Bcl-2/Bax expression of human hepatoma SMMC-7721 cells after exposure to heavy-ion and X-ray irradiations[C]. 见:1st International Conference on Applications of Radiotracers in Chemical Environmental and Biological Sciences. Kolkata, INDIA. JAN 23-27, 2006.
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