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题名: Interaction of IVIG and camptothecin
作者: Liu, Yong-Chun; Wei, Xiao-Xia; Xu, Xiu-Ying; Yao, XJ(姚小军); Lei, Rui-Xia; Zheng, Xu-Dong; Liu, Jian-Ning
收录类别: EI
出版日期: 2015
会议名称: Proceedings of the 2014 International Conference on Medicine Sciences and Bioengineering, ICMSB 2014
会议日期: August 16, 2014 - August 17, 2014
会议地点: Kunming, Yunnan, China
英文摘要: The interaction of camptothecin (CPT) with intravenous immunoglobulin (IVIG) was studied in vitro by fluorescence spectra, Fourier Transformation Infrared (FT-IR) spectra and molecular docking. The binding parameters calculated according to the Sips equation suggested that the binding of IVIG to CPT was a spontaneous entropy driven, non-specific and weak drug-protein interaction, characterized by two binding sites with the average affinity constant Ko of 9.683 × 103 L·mol-1 at pH 4.0 and 310 K. The contents of secondary structural composition of free IVIG and its CPT complex were calculated by FT-IR difference spectra, self-deconvolution, second derivative resolution enhancement and the curve-fitting procedure of amide I band, respectively. The observed spectral changes indicated a partial unfolding of the typical β structure of protein. Molecular docking was used to calculate the interaction mode between CPT with human IgG and the docking result of ΔG° (-22.11 KJ·mol-1) was consistent with that of Sips method. IVIG can serve as a transport protein (carrier) for CPT. © 2015 Taylor & Francis Group, London.
关键词: Binding sites ; Body fluids ; Curve fitting ; Fourier transforms ; Molecular modeling ; Proteins ; Affinity constants ; Drug-protein interactions ; Fluorescence spectra ; Fourier transformations ; Resolution enhancement ; Second derivatives ; Self-deconvolution ; Structural composition
作者部门: (1) College of Chemistry and Chemical Engineering, Qibo College of Medicine, Cooperative Innovation Center of Industrial Surfactant, Longdong University, Qingyang ; Gansu, China ; (2) College of Chemistry and Chemical Engineering, State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, China
通讯作者: Liu, Yong-Chun
学科分类: Biochemistry;Organic Compounds; Mathematics;Atomic and Molecular Physics
会议录: Medicine Sciences and Bioengineering - Proceedings of the 2014 International Conference on Medicine Sciences and Bioengineering, ICMSB 2014
页码: 551-558
出版者: CRC Press
语种: 英语
EI记录号: 20153601247107
IR记录号: 20153601247107
第一机构:
内容类型: 会议论文
URI标识: http://ir.lzu.edu.cn/handle/262010/185194
Appears in Collections:化学化工学院_会议论文

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Recommended Citation:
Liu, Yong-Chun,Wei, Xiao-Xia,Xu, Xiu-Ying,et al. Interaction of IVIG and camptothecin[C]. 见:Proceedings of the 2014 International Conference on Medicine Sciences and Bioengineering, ICMSB 2014. Kunming, Yunnan, China. August 16, 2014 - August 17, 2014.
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