兰州大学机构库 >基础医学院
Absence of Protoheme IX Farnesyltransferase CtaB Causes Virulence Attenuation but Enhances Pigment Production and Persister Survival in MRSA
Xu, T; Han, J; Zhang, J; Chen, J; Wu, N; Zhang, W; Zhang, Y; Zhang, Y (reprint author), Fudan Univ, Shanghai Med Coll, Huashan Hosp, Key Lab Med Mol Virol, Shanghai, Peoples R China.; Zhang, Y (reprint author), Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Mol Microbiol & Immunol, Baltimore, MD 21218 USA.
Indexed BySCIE ; PubMed
2016-10-24
Source PublicationFRONTIERS IN MICROBIOLOGY
Volume7
PublisherFRONTIERS MEDIA SA
Publication PlaceLAUSANNE
AbstractThe membrane protein CtaB in S. aureus is a protoheme IX farnesyltransferase involved in the synthesis of the heme containing terminal oxidases of bacterial respiratory chain. In this study, to assess the role of CtaB in S. aureus virulence, pigment production, and persister formation, we constructed a ctaB mutant in the methicillin-resistant Staphylococcus aureus (MRSA) strain USA500. We found that deletion of ctaB attenuated growth and virulence in mice but enhanced pigment production and formation of quinolone tolerant persister cells in stationary phase. RNA-seq analysis showed that deletion of ctaB caused decreased transcription of several virulence genes including RNAIII which is consistent with its virulence attenuation. In addition, transcription of 20 ribosomal genes and 24 genes involved in amino acid biosynthesis was significantly down-regulated in the ctaB knockout mutant compared with the parent strain. These findings suggest the importance of heme biosynthesis in virulence and persister formation of S. aureus.
KeywordStaphylococcus aureus heme antibiotics pigment virulence persister formation
Department[Xu, Tao;
Zhang, Jia;
Chen, Jiazhen;
Wu, Nan;
Zhang, Wenhong;
Zhang, Ying] Fudan Univ, Shanghai Med Coll, Huashan Hosp, Key Lab Med Mol Virol, Shanghai, Peoples R China;
[Han, Jian] Lanzhou Univ, Sch Basic Med Sci, Dept Pathogen Biol, Lanzhou, Peoples R China;
[Zhang, Ying] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Mol Microbiol & Immunol, Baltimore, MD 21218 USA
Subject AreaMicrobiology
Project NumberNational Natural Science Foundation of China [81572046, 81471987] ; Key Technologies Research and Development Program for Infectious Diseases of China [2013ZX10003008-003] ; NIH [AI99512, AI108535]
Funding Project国家自然科学基金项目 ; 国家科技重大专项 ; 美国国立卫生研究院项目
Funding OrganizationNSFC ; MOST ; NIH
DOI10.3389/fmicb.2016.01625
ISSN1664-302X
SubtypeArticle
Language英语
WOS IDWOS:000388631700001
PMID 27822202
IRIDWOS:000388631700001
First Inst
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.lzu.edu.cn/handle/262010/188736
Collection基础医学院
Corresponding AuthorZhang, W; Zhang, Y (reprint author), Fudan Univ, Shanghai Med Coll, Huashan Hosp, Key Lab Med Mol Virol, Shanghai, Peoples R China.; Zhang, Y (reprint author), Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Mol Microbiol & Immunol, Baltimore, MD 21218 USA.
Recommended Citation
GB/T 7714
Xu, T,Han, J,Zhang, J,et al. Absence of Protoheme IX Farnesyltransferase CtaB Causes Virulence Attenuation but Enhances Pigment Production and Persister Survival in MRSA[J]. FRONTIERS IN MICROBIOLOGY,2016,7.
APA Xu, T.,Han, J.,Zhang, J.,Chen, J.,Wu, N.,...&Zhang, Y .(2016).Absence of Protoheme IX Farnesyltransferase CtaB Causes Virulence Attenuation but Enhances Pigment Production and Persister Survival in MRSA.FRONTIERS IN MICROBIOLOGY,7.
MLA Xu, T,et al."Absence of Protoheme IX Farnesyltransferase CtaB Causes Virulence Attenuation but Enhances Pigment Production and Persister Survival in MRSA".FRONTIERS IN MICROBIOLOGY 7(2016).
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