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兰州大学机构库  > 草地农业科技学院  > 期刊论文
题名: Development of a Real-time Fluorescence Loop-mediated Isothermal Amplification Assay for Detection of Burkholderia gladioli pv. alliicola
作者: Kuang, WG; Luo, LX; Gao, WN; Lei, YH; Lv, QY; Li, JQ
收录类别: SCIE
出版日期: 2017-02
刊名: JOURNAL OF PHYTOPATHOLOGY
卷号: 165, 期号:1-2, 页码:82-90
出版者: WILEY
出版地: HOBOKEN
英文摘要: Burkholderia gladioli pv. alliicola is a causal agent of rot on a wide range of hosts including onion and tulip. It is one of quarantine phytopathogenic bacteria in China. To reduce the economic losses associated with this pathogen, simple and rapid detection methods are needed. In this study, an efficient loop-mediated isothermal amplification (LAMP) assay with a real-time fluorometer was developed. The analysis of 16S-23S rRNA intergenic transcribed spacer (ITS) sequences showed considerable variability between different Burkholderia species and B.gradioli pathovars. A set of LAMP primers was designed based on the ITS region. The sensitivity and specificity of the developed assay were evaluated at the optimal temperature of 65 degrees C. The primers were specific for B.gladioli pv. alliicola and did not react to strains of others species and other pathovars in the species B.gladioli. The sensitivity of the real-time LAMP assay was 1fg DNA which was 100 times higher than that of conventional PCR. The method was verified by testing natural samples and inoculated onion seeds, and it showed effectiveness. The real-time LAMP assay established in this study is an effective method for detection of B.gladioli pv. alliicola.
关键词: Burkholderia ; detection ; LAMP ; sequence analysis
作者部门: [Kuang, Weigang ; Luo, Laixin ; Lv, Qingyang ; Li, Jianqiang] China Agr Univ, Dept Plant Pathol, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R China ; [Kuang, Weigang ; Gao, Wenna] Beijing Entry Exit Inspect & Quarantine Bur, Beijing 100026, Peoples R China ; [Lei, Yahong] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Key Lab Grassland Farming Syst, Lanzhou 730020, Peoples R China
通讯作者: Li, JQ (reprint author), China Agr Univ, Dept Plant Pathol, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R China. ; Gao, WN (reprint author), Beijing Inspect & Quarantine Testing Ctr, Beijing, Peoples R China.
学科分类: Plant Sciences
文章类型: Article
所属项目编号: National Quality Supervision and Inspection Bureau of Science and Technology planning project of China [2014IK005] ; National 'Twelfth Five-Year' Plan for Science and Technology Support Program of China [2012BAK11B02] ; Seed Health Center of China Agricultural University
所属项目名称: 国家科技支撑计划
项目资助者: MOST
语种: 英语
DOI: 10.1111/jph.12539
ISSN号: 0931-1785
WOS记录号: WOS:000393807000002
IR记录号: WOS:000393807000002
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/188798
Appears in Collections:草地农业科技学院_期刊论文

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Recommended Citation:
Kuang, WG,Luo, LX,Gao, WN,et al. Development of a Real-time Fluorescence Loop-mediated Isothermal Amplification Assay for Detection of Burkholderia gladioli pv. alliicola[J]. JOURNAL OF PHYTOPATHOLOGY,2017,165(1-2):82-90.
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