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题名: The characterization of 1-(4-bromophenyl)-5-phenyl-1H-1,2,3-triazole on acute toxicity, antimicrobial activities, photophysical property, and binding to two globular proteins
作者: Liu, H; Lin, Q; Xie, YX; Shu, HM; Li, B; Gao, G; Xiao, K; Yao, XJ(姚小军); Dong, RC; Liu, YL; He, MX; Wu, LY; Sun, ZF; He, WY
收录类别: SCIE ; PubMed ; MEDLINE
出版日期: 2016-11
刊名: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
卷号: 164, 页码:191-203
出版者: ELSEVIER
出版地: LAUSANNE
英文摘要: 1-(4-Bromophenyl)-5-phenyl-1H-1,2,3-triazole (BPT) was a newly synthesized compound. The acute toxicities of BPT to mice by intragastric administration have been determined and the result indicates that the intragastric administration of BPT did not produce any significant toxic effect on Kunming strain mice. It is also evaluated for the antimicrobial activity of BPT against three kinds of plant mycoplasma, Fusarium Wilt (race 4), Colletotrichum gloeosporioides Penz. and Xanthomonas oryzae by different method in vitro. The compound exhibited distinct inhibitory activities against Fusarium Wilt (race 4) and Colletotrichum gloeosporioides Penz. by mycelium growth rate test and the values of EC50 were 2934 and 12.53 mu g/mL respectively. And BPT had also the most potent inhibitory activities against Xanthomonas oryzae when compared with that of control drugs by the agar well diffusion method. In addition, the structural and photophysical properties of BPT including ionization energy, electron affinities, and theoretical spectrum was studied by quantum-chemical methods. Then the interaction of BPT with two kinds of globular proteins, human immunoglobulin (HIg) and bovine hemoglobin (BHg) was investigated by using UV-vis absorption spectra, synchronous fluorescence, 3D fluorescence spectra, and fluorescence titration in combination with molecular modeling. UV-vis absorption, 3D and synchronous fluorescence measurements show that BPT has influence on the microenvironment surrounding HIg or BHg in aqueous solution and the fluorescence experiments show that BPT quenches the fluorescence intensity of HIg or BHg through a static mechanism. The binding parameters including the binding constants, the number of binding site and average binding distance between BPT and HIg or BHg at different temperatures were calculated. The thermodynamic parameters suggest that the hydrophobic interaction is the predominant intermolecular forces in stabilizing the BPT-HIg or BPT-BHg complex. Molecular docking was performed to reveal that the BPT moiety binds to the hydrophobic cavity of HIg or BHg and they are in good agreement with the spectroscopic measurements. (C) 2016 Elsevier B.V. All rights reserved.
关键词: BPT ; Acute toxicity ; Antimicrobial activities ; HIg ; BHg ; Binding
作者部门: [Liu, He ; Lin, Qiang ; Li, Bo ; Gao, Ge ; Xiao, Kai ; Liu, Yanlin ; He, Mengxiong ; Wu, Luyong ; Sun, Zhenfan ; He, Wenying] Hainan Normal Univ, Minist Educ, Key Lab Trop Med Plant Chem, Haikou 571158, Hainan, Peoples R China ; [Xie, Yixian] Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Key Lab Integrated Pest Management Trop Crops, Haikou 571158, Hainan, Peoples R China ; [Shu, Huoming] Hainan Coll Econ & Business, Haikou 571127, Hainan, Peoples R China ; [Yao, Xiaojun] Lanzhou Univ, Coll Chem & Chem Engn, Lanzhou 730000, Peoples R China ; [Dong, Runcong] Res Ctr Drug Safety Evaluat Hainan Prov, Haikou 571158, Hainan, Peoples R China
通讯作者: He, WY (reprint author), Hainan Normal Univ, Minist Educ, Key Lab Trop Med Plant Chem, Haikou 571158, Hainan, Peoples R China.
学科分类: Biochemistry & Molecular Biology; Biophysics
文章类型: Article
所属项目编号: Fund for the National Natural Science Foundation of China [21562019, 21362008] ; Science and Technology Plan Projects of Hainan Province of China [ZDXM2014023] ; Hainan Province Natural Science Foundation of innovative research team project [2016CXTD007] ; National Undergraduate Innovative Training Program [201411658033]
所属项目名称: 国家自然科学基金项目
项目资助者: NSFC
语种: 英语
DOI: 10.1016/j.jphotobiol.2016.09.029
ISSN号: 1011-1344
WOS记录号: WOS:000387626400023
PM记录号: 27693762
IR记录号: WOS:000387626400023
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/188880
Appears in Collections:化学化工学院_期刊论文

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Recommended Citation:
Liu, H,Lin, Q,Xie, YX,et al. The characterization of 1-(4-bromophenyl)-5-phenyl-1H-1,2,3-triazole on acute toxicity, antimicrobial activities, photophysical property, and binding to two globular proteins[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,2016,164:191-203.
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