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The characterization of 1-(4-bromophenyl)-5-phenyl-1H-1,2,3-triazole on acute toxicity, antimicrobial activities, photophysical property, and binding to two globular proteins
Liu, H; Lin, Q; Xie, YX; Shu, HM; Li, B; Gao, G; Xiao, K; Yao, XJ(姚小军); Dong, RC; Liu, YL; He, MX; Wu, LY; Sun, ZF; He, WY; He, WY (reprint author), Hainan Normal Univ, Minist Educ, Key Lab Trop Med Plant Chem, Haikou 571158, Hainan, Peoples R China.
2016-11
发表期刊JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
ISSN1011-1344
卷号164页码:191-203
出版者ELSEVIER
摘要1-(4-Bromophenyl)-5-phenyl-1H-1,2,3-triazole (BPT) was a newly synthesized compound. The acute toxicities of BPT to mice by intragastric administration have been determined and the result indicates that the intragastric administration of BPT did not produce any significant toxic effect on Kunming strain mice. It is also evaluated for the antimicrobial activity of BPT against three kinds of plant mycoplasma, Fusarium Wilt (race 4), Colletotrichum gloeosporioides Penz. and Xanthomonas oryzae by different method in vitro. The compound exhibited distinct inhibitory activities against Fusarium Wilt (race 4) and Colletotrichum gloeosporioides Penz. by mycelium growth rate test and the values of EC50 were 2934 and 12.53 mu g/mL respectively. And BPT had also the most potent inhibitory activities against Xanthomonas oryzae when compared with that of control drugs by the agar well diffusion method. In addition, the structural and photophysical properties of BPT including ionization energy, electron affinities, and theoretical spectrum was studied by quantum-chemical methods. Then the interaction of BPT with two kinds of globular proteins, human immunoglobulin (HIg) and bovine hemoglobin (BHg) was investigated by using UV-vis absorption spectra, synchronous fluorescence, 3D fluorescence spectra, and fluorescence titration in combination with molecular modeling. UV-vis absorption, 3D and synchronous fluorescence measurements show that BPT has influence on the microenvironment surrounding HIg or BHg in aqueous solution and the fluorescence experiments show that BPT quenches the fluorescence intensity of HIg or BHg through a static mechanism. The binding parameters including the binding constants, the number of binding site and average binding distance between BPT and HIg or BHg at different temperatures were calculated. The thermodynamic parameters suggest that the hydrophobic interaction is the predominant intermolecular forces in stabilizing the BPT-HIg or BPT-BHg complex. Molecular docking was performed to reveal that the BPT moiety binds to the hydrophobic cavity of HIg or BHg and they are in good agreement with the spectroscopic measurements. (C) 2016 Elsevier B.V. All rights reserved.
出版地LAUSANNE
关键词BPT Acute toxicity Antimicrobial activities HIg BHg Binding
学科领域Biochemistry & Molecular Biology ; Biophysics
作者部门
[Liu, He;
Lin, Qiang;
Li, Bo;
Gao, Ge;
Xiao, Kai;
Liu, Yanlin;
He, Mengxiong;
Wu, Luyong;
Sun, Zhenfan;
He, Wenying] Hainan Normal Univ, Minist Educ, Key Lab Trop Med Plant Chem, Haikou 571158, Hainan, Peoples R China...更多
DOI10.1016/j.jphotobiol.2016.09.029
文章类型Article
收录类别SCIE ; PubMed ; MEDLINE
所属项目编号Fund for the National Natural Science Foundation of China [21562019, 21362008] ; Science and Technology Plan Projects of Hainan Province of China [ZDXM2014023] ; Hainan Province Natural Science Foundation of innovative research team project [2016CXTD007] ; National Undergraduate Innovative Training Program [201411658033]
语种英语
资助项目国家自然科学基金项目
项目资助者NSFC
WOS记录号WOS:000387626400023
PM记录号27693762
IR记录号WOS:000387626400023
第一机构
Citation statistics
文献类型期刊论文
条目标识符http://ir.lzu.edu.cn/handle/262010/188880
Collection化学化工学院
通讯作者He, WY (reprint author), Hainan Normal Univ, Minist Educ, Key Lab Trop Med Plant Chem, Haikou 571158, Hainan, Peoples R China.
Recommended Citation:
GB/T 7714
Liu, H,Lin, Q,Xie, YX,et al. The characterization of 1-(4-bromophenyl)-5-phenyl-1H-1,2,3-triazole on acute toxicity, antimicrobial activities, photophysical property, and binding to two globular proteins[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,2016,164:191-203.
APA Liu, H.,Lin, Q.,Xie, YX.,Shu, HM.,Li, B.,...&He, WY .(2016).The characterization of 1-(4-bromophenyl)-5-phenyl-1H-1,2,3-triazole on acute toxicity, antimicrobial activities, photophysical property, and binding to two globular proteins.JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,164,191-203.
MLA Liu, H,et al."The characterization of 1-(4-bromophenyl)-5-phenyl-1H-1,2,3-triazole on acute toxicity, antimicrobial activities, photophysical property, and binding to two globular proteins".JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 164(2016):191-203.
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