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题名: Characterization of Nitrate-Reducing and Amino Acid-Using Bacteria Prominent in Nitrotoxin-Enriched Equine Cecal Populations
作者: Zhang, Y; Long, RJ; Anderson, RC; Humec, ME; Coverdale, JA; Latham, EA; Nisbet, DJ
收录类别: SCIE
出版日期: 2016-11
刊名: JOURNAL OF EQUINE VETERINARY SCIENCE
卷号: 46, 页码:47-53
出版者: ELSEVIER
出版地: NEW YORK
英文摘要: Horses can be at risk for nitropoisoning by consuming plants containing 3-nitro-1-propionic acid or 3-nitro-1-propanol and to a lesser extent by plants containing nitrate. Populations of equine cecal microbes enriched for enhanced rates of 3-nitro-1-propionic acid (NPA) or nitrate metabolism were cultured for NPA- or nitrate-metabolizing bacteria on basal enrichment medium or tryptose soy agar supplemented with either 5-mM NPA or nitrate and under H-2:CO2 (20:80) as the energy source. After 72 hours, separated colonies picked from plates, or roll tubes were cultured in fresh broth medium for 72 hours and then identified by 16S rRNA gene sequencing. Isolates from the NPA-enriched populations were identified as Streptococcus lutetiensis (five strains), Escherichia coli (two strains), and Sporanaerobacter acetigens (one strain). Strains isolated from nitrate-enriched populations were identified as Escherichia coli (one strain) and Wolinella succinogenes (three strains). None of these strains degraded NPA. Enriched populations of equine cecal microbes, the isolated pure strains and the type strain of Denitrobacterium detoxificans, a competent NPA-metabolizing microbe, were examined using denaturing gradient gel electrophoresis (DGGE). The DGGE analysis indicated that none of the strains in the enriched population of equine cecal microbes were similar to D. detoxificans. However, we report for the first time the isolation of the anaerobic amino acid-using Sporanaerobacter acetigenes from the equine cecum. Published by Elsevier Inc.
关键词: Equine ; Nitrotoxin metabolism ; DGGE ; Sporanaerobacter acetigens
作者部门: [Zhang, Ying] Lanzhou Univ, Sch Publ Hlth, Lanzhou, Peoples R China ; [Long, Ruijun] Lanzhou Univ, Sch Life Sci, Lanzhou, Peoples R China ; [Anderson, Robin C. ; Humec, Michael E. ; Nisbet, David J.] ARS, USDA, College Stn, TX USA ; [Coverdale, Josie A. ; Latham, Elizabeth A.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA
通讯作者: Anderson, RC (reprint author), ARS, USDA, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, 2881 F&B Rd, College Stn, TX 77845 USA.
学科分类: Veterinary Sciences
文章类型: Article
所属项目编号: National Natural Science Foundation of China [31501976] ; Fundamental Research Funds for the Central Universities [lzujbky-2015-183, lzujbky-2015-261]
所属项目名称: 国家自然科学基金项目 ; 中央高校基本科研业务费专项资金
项目资助者: NSFC ; LZU
语种: 英语
DOI: 10.1016/j.jevs.2016.04.007
ISSN号: 0737-0806
WOS记录号: WOS:000386826200008
IR记录号: WOS:000386826200008
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/189117
Appears in Collections:公共卫生学院_期刊论文

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Recommended Citation:
Zhang, Y,Long, RJ,Anderson, RC,et al. Characterization of Nitrate-Reducing and Amino Acid-Using Bacteria Prominent in Nitrotoxin-Enriched Equine Cecal Populations[J]. JOURNAL OF EQUINE VETERINARY SCIENCE,2016,46:47-53.
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