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题名: Reelin and cofilin cooperate during the migration of cortical neurons: a quantitative morphological analysis
作者: Chai, XJ; Zhao, ST; Fan, L; Zhang, W; Lu, X; Shao, H; Wang, SB; Song, LZ; Failla, AV; Zobiak, B; Mannherz, HG; Frotscher, M
收录类别: SCIE ; PubMed ; MEDLINE
出版日期: 2017-03-15
刊名: DEVELOPMENT
卷号: 143, 期号:1-6, 页码:1029-1040
出版者: COMPANY OF BIOLOGISTS LTD
出版地: CAMBRIDGE
英文摘要: In reeler mutant mice, which are deficient in reelin (Reln), the lamination of the cerebral cortex is disrupted. Reelin signaling induces phosphorylation of LIM kinase 1, which phosphorylates the actin-depolymerizing protein cofilin in migrating neurons. Conditional cofilin mutants show neuronal migration defects. Thus, both reelin and cofilin are indispensable during cortical development. To analyze the effects of cofilin phosphorylation on neuronal migration we used in utero electroporation to transfect E14.5 wild-type cortical neurons with pCAG-EGFP plasmids encoding either a nonphosphorylatable form of cofilin 1 (cofilin(S3A)), a pseudophosphorylated form (cofilin(S3E)) or wild-type cofilin 1 (cofilin(WT)). Wild-type controls and reeler neurons were transfected with pCAG-EGFP. Real-time microscopy and histological analyses revealed that overexpression of cofilinWT and both phosphomutants induced migration defects and morphological abnormalities of cortical neurons. Of note, reeler neurons and cofilin(S3A)- and cofilin(S3E)-transfected neurons showed aberrant backward migration towards the ventricular zone. Overexpression of cofilinS3E, the pseudophosphorylated form, partially rescued the migration defect of reeler neurons, as did overexpression of Limk1. Collectively, the results indicate that reelin and cofilin cooperate in controlling cytoskeletal dynamics during neuronal migration.
关键词: Neuronal migration ; Cofilin phosphorylation ; Reelin signaling ; Actin cytoskeleton ; In utero electroporation
作者部门: [Chai, Xuejun ; Zhao, Shanting ; Wang, Shaobo ; Song, Lingzhen ; Frotscher, Michael] Univ Med Ctr Hamburg Eppendorf, Ctr Mol Neurobiol Hamburg ZMNH, Inst Struct Neurobiol, D-20251 Hamburg, Germany ; [Zhao, Shanting ; Zhang, Wei ; Lu, Xi] Northwest A&F Univ, Coll Vet Med, Yangling 712100, Peoples R China ; [Fan, Li ; Shao, Hong] Lanzhou Univ, Sch Life Sci, Inst Zool, Lanzhou 730000, Peoples R China ; [Failla, Antonio Virgilio ; Zobiak, Bernd] Univ Med Ctr Hamburg Eppendorf, UMIF, D-20251 Hamburg, Germany ; [Mannherz, Hans G.] Ruhr Univ Bochum, Inst Anat & Mol Embryol, D-44780 Bochum, Germany
通讯作者: Frotscher, M (reprint author), Univ Med Ctr Hamburg Eppendorf, Ctr Mol Neurobiol Hamburg ZMNH, Inst Struct Neurobiol, D-20251 Hamburg, Germany.
学科分类: Developmental Biology
文章类型: Article
所属项目编号: Deutsche Forschungsgemeinschaft [FR 620/12-2, FR 620/14-1] ; National Natural Science Foundation of China [31071873]
所属项目名称: 国家自然科学基金项目 ; 德国科学基金会项目
项目资助者: NSFC ; DFG
语种: 英语
DOI: 10.1242/dev.134163
ISSN号: 0950-1991
WOS记录号: WOS:000384750600014
PM记录号: 26893343
IR记录号: WOS:000384750600014
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/189425
Appears in Collections:生命科学学院_期刊论文

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Recommended Citation:
Chai, XJ,Zhao, ST,Fan, L,et al. Reelin and cofilin cooperate during the migration of cortical neurons: a quantitative morphological analysis[J]. DEVELOPMENT,2017,143(1-6):1029-1040.
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