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题名: A reversible ratiometric two-photon lysosometargeted probe for real-time monitoring of pH changes in living cells
作者: Luo, WF; Jiang, H; Tang, XL; Liu, WS(刘伟生)
收录类别: SCIE
出版日期: 2017-06-28
刊名: JOURNAL OF MATERIALS CHEMISTRY B
卷号: 5, 期号:24, 页码:4768-4773
出版者: ROYAL SOC CHEMISTRY
出版地: CAMBRIDGE
英文摘要: Lysosome pH is known to be acidic (4.5-5.5) and has emerged as a critical physiological factor for lysosome activities and functions. Herein, we designed a novel ratiometric lysosome-targeted fluorescence resonance energy transfer (FRET) pH probe, which was fabricated by integrating the coumarin moiety (donor) with the naphthalimide moiety (acceptor). The sensing mechanism was essentially an integration of ICT and FRET processes, leading to the simultaneous intensity enhancement of coumarin and naphthalimide with a pH increase. Furthermore, morpholine was introduced as a lysosome-targeted group. Moreover, the probe could respond to pH in a proportional relationship at very broad range from pH 4.5 to 11.0 and showed remarkable advantages, including rapid response, high sensitivity and selectivity, suitable pK(a) of 5.62, and good reversibility. Furthermore, the probe was successfully used as a ratiometric TP lysosome-targeted fluorescence probe, not only for imaging of lysosomal pH, but also for visualizing chloroquine-induced changes of intracellular pH in real time in living cells with low cytotoxicity and autofluorescence. These proof-of concept studies demonstrate the practical application of the probe in biological systems.
作者部门: [Liu, Weisheng] Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China ; Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China
通讯作者: Liu, WS (reprint author), Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China.
学科分类: Materials Science
文章类型: Article
所属项目编号: NSFC [21431002] ; Fundamental Research Funds for the Central Universities [lzujbky-2015-k04]
所属项目名称: 国家自然科学基金项目 ; 中央高校基本科研业务费专项资金
项目资助者: NSFC ; LZU
语种: 英语
DOI: 10.1039/c7tb00838d
ISSN号: 2050-750X
WOS记录号: WOS:000404070600023
IR记录号: WOS:000404070600023
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/190171
Appears in Collections:化学化工学院_期刊论文

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作者单位: 16CHEM

Recommended Citation:
Luo, WF,Jiang, H,Tang, XL,et al. A reversible ratiometric two-photon lysosometargeted probe for real-time monitoring of pH changes in living cells[J]. JOURNAL OF MATERIALS CHEMISTRY B,2017,5(24):4768-4773.
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