兰州大学机构库 >化学化工学院
A label-free colorimetric biosensor for sensitive detection of vascular endothelial growth factor-165
Zhang, HG; Peng, L; Li, MX; Ma, J; Qi, SD; Chen, HL; Zhou, L; Chen, XG(陈兴国); Chen, XG (reprint author), Lanzhou Univ, Natl Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China.; Chen, XG (reprint author), Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China.
收录类别SCIE
2017-07-07
发表期刊ANALYST
卷号142期号:13页码:2419-2425
出版者ROYAL SOC CHEMISTRY
出版地CAMBRIDGE
摘要Sensitive detection of a low abundant protein is essential for biomedical research and clinical diagnostics. Herein, we develop a label-free colorimetric biosensor for the sensitive detection of recombinant human vascular endothelial growth factor-165 (VEGF(165)). This biosensor consists of an aptamer-based hairpin probe, an assistant DNA-trigger duplex and a linear template. In the presence of VEGF(165), the specific binding of VEGF(165) with the aptamer-based hairpin probe results in the opening of a hairpin probe and the opened hairpin probe subsequently hybridizes with the single-stranded region of the assistant DNA-trigger duplex to initiate the strand displacement amplification (SDA) to yield abundant triggers. The released triggers can further function as the primers to anneal with the hairpin probe and lead to the opening of the hairpin structure, which subsequently hybridizes with the assistant DNA-trigger duplex to initiate the next round of SDA reaction and generates more triggers. Large amounts of triggers could be generated by the synergistic operation of dual SDA reaction, and the obtained triggers can initiate a new round of SDA reaction to yield numerous G-quadruplex DNAzymes, which subsequently catalyze the conversion of ABTS(2-) to ABTS(center dot-) by H2O2 to yield a color change with the assistance of a cofactor hemin. In contrast, in the absence of target VEGF(165), the hairpin probe, the assistant DNA-trigger duplex and the linear template can stably coexist in solution, and thus no color change is observed because no trigger can initiate SDA to generate the G-quadruplex DNAzyme. This biosensor has a low detection limit of 1.70 pM and a dynamic range over 3 orders of magnitude from 24.00 pM to 11.25 nM. Moreover, the biosensor shows excellent specificity toward the target VEGF(165) and the entire reaction can be carried out in an isothermal manner without the involvement of a high precision thermal cycler, making the current assay extremely cost effective.
作者部门
[Zhang, Huige;
Qi, Shengda;
Chen, Hongli;
Zhou, Lei;
Chen, Xingguo] Lanzhou Univ, Natl Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China;
[Zhang, Huige;
Qi, Shengda;
Chen, Hongli;
Zhou, Lei;
Chen, Xingguo] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China...更多
学科领域Chemistry
所属项目编号National Natural Science Foundation of China [21405066, 21375053, 21475053] ; Fundamental Research Funds for the Central Universities [lzujbky-2016-40]
资助项目国家自然科学基金项目 ; 中央高校基本科研业务费专项资金
项目资助者NSFC ; LZU
DOI10.1039/c7an00541e
ISSN0003-2654
文章类型Article
语种英语
WOS记录号WOS:000404274500015
IR记录号WOS:000404274500015
第一机构
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文献类型期刊论文
条目标识符http://ir.lzu.edu.cn/handle/262010/190176
Collection化学化工学院
通讯作者Chen, XG (reprint author), Lanzhou Univ, Natl Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China.; Chen, XG (reprint author), Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China.
作者单位16CHEM
Recommended Citation:
GB/T 7714
Zhang, HG,Peng, L,Li, MX,et al. A label-free colorimetric biosensor for sensitive detection of vascular endothelial growth factor-165[J]. ANALYST,2017,142(13):2419-2425.
APA Zhang, HG.,Peng, L.,Li, MX.,Ma, J.,Qi, SD.,...&Chen, XG .(2017).A label-free colorimetric biosensor for sensitive detection of vascular endothelial growth factor-165.ANALYST,142(13),2419-2425.
MLA Zhang, HG,et al."A label-free colorimetric biosensor for sensitive detection of vascular endothelial growth factor-165".ANALYST 142.13(2017):2419-2425.
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