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题名: A label-free colorimetric biosensor for sensitive detection of vascular endothelial growth factor-165
作者: Zhang, HG; Peng, L; Li, MX; Ma, J; Qi, SD; Chen, HL; Zhou, L; Chen, XG(陈兴国)
收录类别: SCIE
出版日期: 2017-07-07
刊名: ANALYST
卷号: 142, 期号:13, 页码:2419-2425
出版者: ROYAL SOC CHEMISTRY
出版地: CAMBRIDGE
英文摘要: Sensitive detection of a low abundant protein is essential for biomedical research and clinical diagnostics. Herein, we develop a label-free colorimetric biosensor for the sensitive detection of recombinant human vascular endothelial growth factor-165 (VEGF(165)). This biosensor consists of an aptamer-based hairpin probe, an assistant DNA-trigger duplex and a linear template. In the presence of VEGF(165), the specific binding of VEGF(165) with the aptamer-based hairpin probe results in the opening of a hairpin probe and the opened hairpin probe subsequently hybridizes with the single-stranded region of the assistant DNA-trigger duplex to initiate the strand displacement amplification (SDA) to yield abundant triggers. The released triggers can further function as the primers to anneal with the hairpin probe and lead to the opening of the hairpin structure, which subsequently hybridizes with the assistant DNA-trigger duplex to initiate the next round of SDA reaction and generates more triggers. Large amounts of triggers could be generated by the synergistic operation of dual SDA reaction, and the obtained triggers can initiate a new round of SDA reaction to yield numerous G-quadruplex DNAzymes, which subsequently catalyze the conversion of ABTS(2-) to ABTS(center dot-) by H2O2 to yield a color change with the assistance of a cofactor hemin. In contrast, in the absence of target VEGF(165), the hairpin probe, the assistant DNA-trigger duplex and the linear template can stably coexist in solution, and thus no color change is observed because no trigger can initiate SDA to generate the G-quadruplex DNAzyme. This biosensor has a low detection limit of 1.70 pM and a dynamic range over 3 orders of magnitude from 24.00 pM to 11.25 nM. Moreover, the biosensor shows excellent specificity toward the target VEGF(165) and the entire reaction can be carried out in an isothermal manner without the involvement of a high precision thermal cycler, making the current assay extremely cost effective.
作者部门: [Zhang, Huige ; Qi, Shengda ; Chen, Hongli ; Zhou, Lei ; Chen, Xingguo] Lanzhou Univ, Natl Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China ; [Zhang, Huige ; Qi, Shengda ; Chen, Hongli ; Zhou, Lei ; Chen, Xingguo] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China ; [Peng, Liang] Lanzhou Univ, Sch Life Sci, Facil Ctr Life Sci Res, Lanzhou 730000, Peoples R China ; [Li, Maoxing ; Ma, Ji] PLA, Lanzhou Gen Hosp, Lanzhou 730050, Peoples R China
通讯作者: Chen, XG (reprint author), Lanzhou Univ, Natl Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China. ; Chen, XG (reprint author), Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China.
学科分类: Chemistry
文章类型: Article
所属项目编号: National Natural Science Foundation of China [21405066, 21375053, 21475053] ; Fundamental Research Funds for the Central Universities [lzujbky-2016-40]
所属项目名称: 国家自然科学基金项目 ; 中央高校基本科研业务费专项资金
项目资助者: NSFC ; LZU
语种: 英语
DOI: 10.1039/c7an00541e
ISSN号: 0003-2654
WOS记录号: WOS:000404274500015
IR记录号: WOS:000404274500015
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/190176
Appears in Collections:化学化工学院_期刊论文

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作者单位: 16CHEM

Recommended Citation:
Zhang, HG,Peng, L,Li, MX,et al. A label-free colorimetric biosensor for sensitive detection of vascular endothelial growth factor-165[J]. ANALYST,2017,142(13):2419-2425.
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