|Alternative Title||Probiotic Lactobacillus salivarius strains’ Screening and a primary research of its antibiotics and
|Place of Conferral||兰州
结果：1. 本实验成功筛选出目标菌株并命名为 w22a。2. (1) w22a产出的抑菌物质随着浓度的增加其抑菌作用开始增强。(2)w22a产出的抑菌物质有一定的热稳定性。(3)w22a的抑菌物质对广谱蛋白酶敏感。（4）w22a产出的抑菌物质对多种口腔常见的致病菌有着程度不一的抑制作用。3. w22a在体外有着良好的粘附能力。
|Other Abstract||Objectives: This study aimed to screen the probiotic potential of Lactobacillus salivarius strain from oral cavity of healthy volunteers, and to investigate its probiotic properties against oral diseases for providing a preliminary strategy.
Methods: (1) Selecting Volunteers that healthy and have not any oral disease, then gathering their unstimulated saliva to selective culture using the LBS solid medium. Selecting the withe and different size circular bacterial colonies to monoclonal cultivate. Lactobacillus salivarius are selected by the Lactobacillus strains’ Gram staining and 16srDNA sequencing and BLAST in NCBI. Moreover, Lactobacillus salivarius strains are cutruled by liquid MRS medium and gathering their fermentation supernatant by high speed Centrifugation and filtration. Using fermentation supernatant to inhibit the streptococcus mutans(SM) and selecting the aim strains which have the significant ability of bacteriostasis. (2) Gathering the aim strain’s fermentation supernatant and detect bactericidal substance’s effect of bacteriostasis in different concentration. We also should detecte its stability to temperature, the sensitivity to protease, and broad-spectrum antibacterial ability. (3) The aim strains and SM UA159 are co-cultured upon the cover glass by different concentration. Using the light microscope and scanning electron microscope(SEM) to observe their adhesive ability in vitro.
Results: 1. We have Selected the 4 Lactobacillus salivarius strains that have different genotype and find they have different bacteriostatic ability for SM UA159. One strain is determined the targeted species which have a significant effect of inhibiting SM UA159 and named w22a. 2. (1) Bacteriostatic ability is not obvious when the w22a’s sterile fermented supernatant diluted 1/3 as stoste. The diameter of inhibition zone have no significant differences compared with controlled group (P＞0.05). But The effect of inhibiting streptococci mutans showed an increasing trends with the antibacterial substance’s concentration. (2) The w22a’s sterile fermented supernatant is treated by different temperature between 60℃ to 90℃ and it’s ability of inhibit SM UA159 have no influence, there are no significant differences between each groups(P＞0.05). (3) The w22a’s sterile fermented supernatant is treated by catalase and other broad-spectrum proteases and it’s ability of inhibit SM UA159 have a significant destroying. The effect of Proteinase K is more s...|
王立鹤. 益生性唾液乳杆菌的筛选及其抑菌物质和粘附作用的初步探究[D]. 兰州. 兰州大学,2016.
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