|Alternative Title||experiment study on neuron proliferation and repairing effect of bone marrow mesenchymal stem cells on rabbit
|Place of Conferral||兰州
|Abstract||国内外很多研究发现骨髓间充质干细胞（Bone marrow mesenchymal stem cells, BMMSCs）脑内移植能够治疗中枢神经系统疾患，本课题主要通过体外的细胞试验研究，进一步探讨BMMSCs在神经元增殖与修复中的作用。
目的：研究BMMSCs诱导分化为神经样细胞的最佳方案，探讨骨髓间充质干细胞条件培养基（BMMSCs conditioned medium, B-CM）诱导神经干细胞分化为神经细胞的可行性及B-CM对损伤神经元的修复作用。
|Other Abstract||Many research suggested that central nervous system disorders could be treated by transplantation of bone marrow mesenchymal stem cells(BMMSCs) in vivo. Further research on effects of bone marrow mesenchymal stem cells on neuron proliferation and repairing effect is to be performed in this study.
Objectives: To investigate the best method of inducing rabbit BMMSCs to differentiate into neuron-like cells，the possibility of bone marrow mesenchymal stem cells inducing neural stem cells to differentiate into neural cells in conditioned-medium (B-CM)and the effects of B-CM on the damaged neuron repairing.
Methods: 30 qingzilan rabbits aged 1 day to 3 months with an average weight of 0.125～1.5kg were used in this study. BMMSCs were harvested from bone marrow and separated using density gradient centrifuge method. BMMSCs cultured and expanded in vitro were induced with gangliosides（GM1）、adenosine triphosphate（ATP）and basic fibroblast growth factor (bFGF ) respectively, and then induced with GM1 /bFGF and ATP/ bFGF. The cultured medium was removed when BMMSCs grew to 90% confluence and then BMMSCs were cultured with serum-free DMEM for 24h and the medium was collected as BMMSCs conditioned-medium(B-CM). Neural stem cells(NSCs) were isolated from the stratium of rabbit and induced by B-CM. The protective effects of B-CM on spinal cord neurons damaged by glutamate were investigated in rabbits. The cells’ viability and repairing degree were assayed by LDH and MMT methods.
Results: The antigen CD44 positively expressed but antigen CD34 negatively expressed on BMMSCs. GM1 and ATP couldn’t induce BMMSCs into neural-like cells but bFGF could do that alone. Further more, GM1 /bFGF and ATP/bFGF promote the differentiation rate respectively. The differentiation rate of nest-like clusters of neural stem cells obtained from stratium of rabbits was increased and the viability of damaged neurons was promoted by B-CM.
Conclusion: The proliferation and repairing of neurons could be improved by BMMSCs.|
李强. 兔骨髓间充质干细胞在神经元增殖与修复中作用的实验研究[D]. 兰州. 兰州大学,2005.
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.