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The DosR antigen Rv1737c from Mycobacterium tuberculosis confers inflammation regulation in tuberculosis infection
Gao, Xiaoling1; Wu, Cong2; Wang, XX(王晓霞)2; Xu, Hui1; Wu, Yu1; Li, Yonghong1; Jia, Yanjuan1; Wei, Chaojun1; He, Wenhua2; Wang, Yongxiang2; Zhang, BZ(张本忠)2
2019-01
Source PublicationSCANDINAVIAN JOURNAL OF IMMUNOLOGY
ISSN0300-9475
Volume89Issue:1
AbstractThere is an urgent need to identify the potential risk factors for activating latent Mycobacterium tuberculosis infection. In this study, we evaluated the immune function of Rv1737c, which is a latency-associated antigen of dormancy survival regulator (DosR) of M. tuberculosis in a mouse model. Our data showed that mice pretreated with recombinant Rv1737c (rRv1737c) exhibited higher levels of antigen-specific antibodies (IgG, IgM and IgA) than sham-treated mice. Following Bacilli Calmette-Guerin (BCG) challenge, rRv1737c adjuvanted with cholera toxin subunit B (CTB) induced diffuse lung inflammation and fibrosis compared to the control mice. The inflammatory pathogenesis due to rRv1737c pre-exposure was associated with a switch in the macrophage phenotype from M1 to activated M2 and was characterized by IL-10 production. Intracellular cytokine analysis further showed that the rRv1737c-pretreated mice exhibited an increased frequency of Th2 cells in the lungs, lymph nodes and spleen after BCG challenge. Furthermore, IFN-gamma expression increased in the lungs after rRv1737c pretreatment compared to that in the sham mice. Accordingly, lung cells from rRv1737c-immunized mice stimulated with killed BCG produced higher levels of multiple cytokines, such as IFN-gamma, IL-10 and IL-6. The results confirmed that the pathological features of rRv1737c promoted inflammation. Overall, our findings provide direct evidence of the pro-inflammatory function of rRv1737c in a murine model of BCG infection, indicating that Rv1737c is a pathogenic antigen of M. tuberculosis and may be key to the recurrence of latent infection.
KeywordDosR antigens M2 macrophages Mycobacterium tuberculosis Rv1737c
DOI10.1111/sji.12729
Indexed BySCIE
Language英语
Funding ProjectNational Natural Science Foundation of China[81360264]
WOS Research AreaImmunology
WOS SubjectImmunology
WOS IDWOS:000454102500004
PublisherWILEY
Original Document TypeArticle
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.lzu.edu.cn/handle/262010/300161
Collection公共卫生学院
Corresponding AuthorGao, Xiaoling; Zhang, Benzhong
Affiliation1.Gansu Prov Hosp, Inst Clin Res & Translat Med, Lanzhou, Gansu, Peoples R China
2.Lanzhou Univ, Sch Publ Hlth, Lanzhou, Gansu, Peoples R China
Corresponding Author AffilicationSchool of Public health
Recommended Citation
GB/T 7714
Gao, Xiaoling,Wu, Cong,Wang, Xiaoxia,et al. The DosR antigen Rv1737c from Mycobacterium tuberculosis confers inflammation regulation in tuberculosis infection[J]. SCANDINAVIAN JOURNAL OF IMMUNOLOGY,2019,89(1).
APA Gao, Xiaoling.,Wu, Cong.,Wang, Xiaoxia.,Xu, Hui.,Wu, Yu.,...&Zhang, Benzhong.(2019).The DosR antigen Rv1737c from Mycobacterium tuberculosis confers inflammation regulation in tuberculosis infection.SCANDINAVIAN JOURNAL OF IMMUNOLOGY,89(1).
MLA Gao, Xiaoling,et al."The DosR antigen Rv1737c from Mycobacterium tuberculosis confers inflammation regulation in tuberculosis infection".SCANDINAVIAN JOURNAL OF IMMUNOLOGY 89.1(2019).
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