兰州大学机构库 >生命科学学院
Effects of Class IIa Bacteriocin-ProducingLactobacillusSpecies on Fermentation Quality and Aerobic Stability of Alfalfa Silage
Li, Fuhou1; Ding, Zitong1; Adesogan, Adegbola T.2; Ke, Wencan1; Jiang, Yun2; Bai, Jie1; Mudassar, Shah1; Zhang, Yixin1; Huang, Wenkang1; Guo, XS(郭旭生)1
2020-09
Source PublicationANIMALS
ISSN2076-2615
Volume10Issue:9
AbstractSimple Summary Bacteriocins produced by lactic acid bacteria are considered good alternatives for feed antibiotics because of inhibiting spoilage microorganisms in silage and non-drug resistance in animals. Owing to the narrow antibacterial spectrum, class I bacteriocin-producing lactic acid bacteria are considered to have limitations as silage inoculants. The research was conducted to evaluate the effects of two class IIa bacteriocin-producingLactobacilluson silage fermentation, microbial population, chemical composition, and aerobic stability. The strains results showed that class IIa bacteriocin-producing lactic acid bacteria could improve silage fermentation quality, reduce counts of molds and yeasts, and improve aerobic stability to a greater extent than inoculation withLactobacillus plantarumMTD/1, a proven, widely used inoculant, which does not produce bacteriocin. The findings of this research are of great value for current understandings and onwards to conduct further research and for possible practical implementation of class IIa bacteriocin-producing lactic acid bacteria as silage inoculants. The effects of two strains of class IIa bacteriocin-producing lactic acid bacteria,Lactobacillus delbrueckiiF17 andLactobacillus plantarum(BNCC 336943), or a non-bacteriocinLactobacillus plantarumMTD/1 (NCIMB 40027), on fermentation quality, microbial counts, and aerobic stability of alfalfa silage were investigated. Alfalfa was harvested at the initial flowering stage, wilted to a dry matter concentration of approximately 32%, and chopped to 1 to 2 cm length. Chopped samples were treated with nothing (control, CON),Lactobacillus delbrueckiiF17 (F17),Lactobacillus plantarum(BNCC 336943) (LPB), orLactobacillus plantarumMTD/1 (NCIMB 40027) (LPN), each at an application rate of 1 x 10(6)colony-forming units/g of fresh weight. Each treatment was ensiled in quadruplicate in vacuum-sealed polyethylene bags packed with 500 g of fresh alfalfa per bag and ensiled at ambient temperature (25 +/- 2 degrees C) for 3, 7, 14, 30, and 60 days. The samples were then subjected to an aerobic stability test after 60 days of ensiling. Compared with the CON silage, the inoculants reduced the pH after 14 days of ensiling. After 60 days, pH was lowest in the LPB-treated silage, followed by the F17 and LPN-treated silages. Inoculation of F17 increased concentrations of lactic acid in silages fermented for 7, 14, 30, and 60 days relative to other treatments, except for the LPN-treated silages ensiled for 30 and 60 days, in which the lactic acid concentrations were similar to that of F17 silage. Application of F17 and LPB decreased the number of yeast and mold relative to CON and LPN-treated silages. Compared with the CON silage, inoculant-treated silages had greater aerobic stability, water-soluble carbohydrate, and crude protein concentrations, and lower neutral detergent fiber, amino acid nitrogen, and ammonia nitrogen concentrations. The LPB-treated silage had the greatest aerobic stability followed by the F17-treated silage. Both class IIa bacteriocin producing inoculants improved alfalfa silage fermentation quality, reduced the growth of yeasts and molds, and improved the aerobic stability of the ensiled forage to a greater extent than the proven LPN inoculant. However, higher crude protein concentration and lower ammonia nitrogen concentration were observed in LPN-treated silage relative to other treatments.
Keywordalfalfa silage bacteriocin aerobic stability lactic acid bacteria fermentation spoilage
DOI10.3390/ani10091575
Indexed BySCIE
Language英语
Funding ProjectNational Natural Science Foundation of China[31872417] ; National Key R&D Program of China[2017YFE0104300]
WOS Research AreaAgriculture ; Veterinary Sciences
WOS SubjectAgriculture, Dairy & Animal Science ; Veterinary Sciences
WOS IDWOS:000580885700001
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.lzu.edu.cn/handle/262010/442112
Collection生命科学学院
Corresponding AuthorGuo, Xusheng
Affiliation1.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Probiot & Biol Feed Res Ctr, Sch Life Sci,State Key Lab Grassland Agroecosyst, Lanzhou 730000, Peoples R China
2.Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA
First Author AffilicationSchool of Life Sciences
Corresponding Author AffilicationSchool of Life Sciences
Recommended Citation
GB/T 7714
Li, Fuhou,Ding, Zitong,Adesogan, Adegbola T.,et al. Effects of Class IIa Bacteriocin-ProducingLactobacillusSpecies on Fermentation Quality and Aerobic Stability of Alfalfa Silage[J]. ANIMALS,2020,10(9).
APA Li, Fuhou.,Ding, Zitong.,Adesogan, Adegbola T..,Ke, Wencan.,Jiang, Yun.,...&Guo, Xusheng.(2020).Effects of Class IIa Bacteriocin-ProducingLactobacillusSpecies on Fermentation Quality and Aerobic Stability of Alfalfa Silage.ANIMALS,10(9).
MLA Li, Fuhou,et al."Effects of Class IIa Bacteriocin-ProducingLactobacillusSpecies on Fermentation Quality and Aerobic Stability of Alfalfa Silage".ANIMALS 10.9(2020).
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