兰州大学机构库
中华羊茅-内生真菌共生体生态学特性的研究
Alternative TitleStudy on the Ecological Characteristics of Epichloë Endophyte-Festuca sinensis Associations
蔺伟虎
Subtype博士
Thesis Advisor南志标
2020-12-10
Degree Grantor兰州大学
Place of Conferral兰州
Degree Discipline草学
Keyword中华羊茅 内生真菌 生物碱 混播和刈割 根际微生物
Abstract中华羊茅(Festuca sinensis)是我国北方高寒地区广泛分布的禾本科优良牧草,其与内生真菌(Epichloë sinensis)形成共生体,显著增加植物的抗逆性。最近的研究表明,该菌为内生真菌新种,对于其与中华羊茅的作用机理尚不明确。本研究以带菌(E+)和不带菌的(E-)中华羊茅为材料,通过温室和田间试验,探究了中华羊茅-内生真菌共生体的产碱特性,混播和刈割对共生体特性影响,以及内生真菌-共生体-土壤微生物三者间的相互关系。旨在为合理利用中华羊茅-内生真菌共生体提供理论基础。获得主要结果如下: (1) 在不同季节(春、夏、秋、冬),对6个生态型(41、57、84、99、111和141)中华羊茅内生真菌共生体产生的3种生物碱(波胺、震颤素和麦角碱)均进行检测。结果表明,共生体产生的波胺含量为18.51 ppm ~ 112.47 ppm,震颤素含量为0.38 ppm ~ 1.15 ppm和麦角碱含量为0.36 ppm ~ 1.13 ppm。在田间和温室条件下,6个生态型中华羊茅内生真菌共生体波胺含量均由春季到秋季呈显著下降(P<0.05);其含量在春季最高,秋季最低。中华羊茅内生真菌共生体产生的波胺含量高于高羊茅(Festuca arundinacea)内生真菌共生体。在田间条件下,5个生态型的中华羊茅内生真菌共生体产生的震颤素含量在秋季最高(P<0.05),春季最低;其麦角碱含量在夏季最高,春季最低。在温室条件,5个生态型中华羊茅内生真菌共生体产生的震颤素含量夏季最高,春季最低(P<0.05);其麦角碱的变化趋势与其在田间的结果一致。中华羊茅内生真菌共生体产生的震颤素和麦角碱含量均低于多年生黑麦草(Lolium perenne)内生真菌共生体。 (2) 在温室(单播, 2:8, 4:6, 6:4, 8:2)和田间(单播, 2:8, 4:6)对中华羊茅(57)与垂穗披碱草进行混播试验。在温室和田间混播比例下,混播均显著促进了中华羊茅和垂穗披碱草的生长(P<0.05);4:6混播比例较其他混播比例显著增加了中华羊茅和垂穗披碱草的株高、分蘖、茎粗和根长(P<0.05);且内生真菌的存在显著增加了中华羊茅和垂穗披碱草的株高和分蘖(P<0.05)。在温室混播比例下,中华羊茅内生真菌共生体波胺含量在8:2混播比例达到最小值,且显著小于其他混播比例(P<0.05),而在田间混播比例下,中华羊茅内生真菌共生体波胺含量在4:6混播比例最低。在温室混播比例下,中华羊茅内生真菌共生体震颤素的含量无显著差异(P>0.05),而在田间条件下,中华羊茅内生真菌共生体震颤素含量在2:8混播比例时最高,且显著高于其他混播比例(P<0.05)。 (3) 在温室条件下,对不同种群E+和E-中华羊茅(111)分别连续刈割了三次结果表明,刈割次数对中华羊茅的营养和品质均有显著影响(P<0.05),在连续1~2次刈割时中华羊茅的营养和品质无显著差异(P>0.05),但在第3次刈割时会显著下降(P<0.05)。除总有机碳含量外,内生真菌的存在能够显著增加中华羊茅的氮、磷、粗蛋白、粗脂肪和粗灰分含量(P<0.05)。

(4) 内生真菌显著增加了中华羊茅的株高、根长、茎粗、根直径、分蘖、叶宽、地上干重和根干重,以及地上氮、根氮、地上磷和根磷含量(P<0.05)。内生真菌显著增加了中华羊茅根系分泌的总氨基酸含量和胆胺含量,显著降低了中华羊茅根系分泌的丙三醇含量(P<0.05)。内生真菌显著增加了中华羊茅根际土壤全氮、全磷、铵态氮、硝态氮、速效磷和速效钾含量,但显著降低了中华羊茅根际土壤有机碳含量和pH (P<0.05)。内生真菌的存在显著降低了中华羊茅根际土壤真菌和细菌群落多样性(P<0.05);中华羊茅根际土壤中,相对丰度最高的真菌群落门是子囊菌门,相对丰度最高的细菌群落门是变形菌门。在种植第二年时,中华羊茅根际土壤真菌群落多样性显著降低,而细菌群落多样性增加(P<0.05)。总体而言,内生真菌对中华羊茅生态型(99)的影响较生态型(111)更为显著。中华羊茅根际土壤理化性质、根系分泌物与根际土壤微生物多样性有显著(P<0.05)相关关系,即根系分泌物总酚酸、总氨基酸、总核酸相对含量与根际土壤真菌多样性呈显著(P<0.05)负相关关系,而与根际土壤细菌多样性呈显著(P<0.05)正相关。内生真菌影响根际土壤微生物群落,可能与根系分泌物和土壤理化性质有关。

Other AbstractFestuca sinensis is one of the good forage in the alpine regions of northern 中文na. It often forms mutually beneficial symbiosis with Epichloë sinensis endophyte, which can increase the stress resistance of host plants. Recent studies have shown that the E. sinensis endophyte is a new species of endophyte. The mechanism of its interaction with F. sinensis is not clear. In the present study, the E+ (infected with endophyte) and E- (endophyte free) plants of F. sinensis have been employed in a series of experiments. The aims were to explore the variations of alkaloid production of E+ F. sinensis in both greenhouse and field during the four seasons of a year, and the impacts of both mixed seeding and cutting management on E. sinensis-F. sinensis associations; and the relationships among the endophyte-symbiont-soil microorganisms. The main findings are as follows: (1) Both in the field and greenhouse, the alkaloids (peramine, lolitrem B and ergot) were detected in six ecotypes E+ F. sinensis (41, 57, 84, 99, 111 and 141) in four seasons (spring, summer, autumn and winter). The results clarified the content range of the three alkaloids in F. sinensis: peramine ranged from 18.51 ppm to 112.47 ppm; lolitrem B ranged from 0.38 ppm to 1.15 ppm and ergot ranged from 0.36 ppm to1.13 ppm. From spring to autumn, both in the field and greenhouse, peramine contents significantly decreased (P < 0.05); its content was highest in spring and lowest in autumn. The content of peramine of endophyte-symbiont in F. sinensis was higher than that in Festuca arundinacea. In the field experiment, the lolitrem B content of five ecotypes F. sinensis was highest in autumn and lowest in spring (P < 0.05); its ergot content was highest in summer and lowest in spring. In the greenhouse experiment, the lolitrem B content of five ecotypes F. sinensis was the highest in summer and the lowest in spring (P < 0.05); the results of their ergot alkaloids were consistent in the field. In addition, the contents of both lolitrem B and ergot of endophyte-symbiont in F. sinensis was lower than in Lolium perenne. (2) Both in the greenhouse (monoculture, 2:8, 4:6, 6:4 and 8:2) and field (monoculture, 2:8 and 4:6) experiments, the mixed sowing of F. sinensis and Elymus nutans were carried out. Both in the greenhouse and field experiments, mixed sowing significantly (P < 0.05) promoted growth of both F. sinensis and E. nutans; the mixed sowing ratio of 4:6 increased growth of both F. sinensis and E. nutans compared with other mixed sowing ratios, such as significantly (P < 0.05) increased the plant height, tillers, stem diameter and root l英语th of both plants; endophyte also significantly (P < 0.05) increased the plant height and tillers of both F. sinensis and E. nutans. In the greenhouse experiment, the peramine of E+ F. sinensis reached lowest at the 8:2 mixed sowing ratio, while in the field experiment, the peramine of E+ F. sinensis were lowest at the 4:6 mixed sowing ratio. In the greenhouse experiment, the lolitrem B of E+ F. sinensis was not different among mixed sowing ratios, while in the field experiment the lolitrem B of E+ F. sinensis was peaked at the 2:8 mixed sowing ratio (P < 0.05). (3) In the greenhouse experiment, the E+ and E- F. sinensis were cut three times, respectively. The results showed that the cuttings had significant impacts on the nutrition and quality of F. sinensis (P < 0.05). Nutrition and quality of F. sinensis had no significant difference between the 1-2 time of cutting, but significantly decreased at the third cutting (P < 0.05). In addition, endophyte significantly increased the nitrogen, phosphorus, crude protein, crude fat and crude ash contents of F. sinensis (P < 0.05). (4) In the greenhouse experiment, the results of a two-year experiment showed that endophyte significantly (P < 0.05) increased the plant height, root l英语th, stem diameter, root diameter, tiller, leaf width, shoot dry weight and root dry weight of F. sinensis, as well as shoot nitrogen, root nitrogen, shoot phosphorus and root phosphorus contents (P < 0.05). Endophyte significantly increased the total amino acid and ethanolamine contents secreted by the roots of F. sinensis, but reduced the glycerol content secreted by the roots of F. sinensis (P < 0.05). Endophyte significantly increased the contents of total nitrogen, total phosphorus, ammonium nitrogen, nitrate nitrogen, available phosphorus and available potassium in the rhizosphere soil of F. sinensis, but significantly reduced the content of organic carbon and pH in the rhizosphere soil of F. sinensis (P < 0.05). Endophyte reduced the fungal and bacterial community diversity in the rhizosphere soil of F. sinensis (P < 0.05); the fungal community with the highest relative abundance was the Ascomycota in the rhizosphere soil; the bacterial community with the highest relative abundance was the Proteobacteria. In the second year of planting, the fungal community diversity decreased, while the bacterial community diversity increased in the rhizosphere soil (P < 0.05). Overall, the impact of endophyte on the ecotype-99 F. sinensis was more significant than that of the ecotype-111 F. sinensis. The rhizosphere soil properties, the root exudates and the rhizosphere soil microbial diversity had a significant correlation (P < 0.05), that is, the relative contents of total phenolic acid, total amino acid, and total nucleic acid in root exudates and the rhizosphere soil fungal diversity were negatively correlated with the diversity of rhizosphere soil fungal (P < 0.05), while positively correlated with the diversity of rhizosphere soil bacterial (P < 0.05). Thus, endophyte promoted the growth of F. sinensis, affected the root exudates and the soil properties, thereby affecting the rhizosphere soil microbial community.
Pages139
URL查看原文
Language中文
Document Type学位论文
Identifierhttp://ir.lzu.edu.cn/handle/262010/448605
Collection兰州大学
Affiliation草地农业科技学院
First Author AffilicationCollege of Pastoral Agriculture Science and Technology
Recommended Citation
GB/T 7714
蔺伟虎. 中华羊茅-内生真菌共生体生态学特性的研究[D]. 兰州. 兰州大学,2020.
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