兰州大学机构库 >公共卫生学院
A highly sensitive immunofluorescence sensor based on bicolor upconversion and magnetic separation for simultaneous detection of fumonisin B1 and zearalenone
Li, Jingzhi1,2; Zhao, Xudong2; Wang, Yu2; Li, Shuang2; Qin, Yingkai2; Han, Tie2; Gao, Zhixian2; Liu, H(刘辉)1
2021-05-21
Source PublicationANALYST
ISSN0003-2654
Volume146Issue:10Pages:3328-3335
AbstractMycotoxins cause significant harm to human health, so it is imperative to develop a highly sensitive and easy-to-operate method for the detection of mycotoxins. Herein, a fluorescence-based magnetic separation immunoassay for simultaneous detection of mycotoxins fumonisin B1 and zearalenone is established. The method employed high fluorescent upconversion-nanoparticles(UCNPs) conjugated with biotinylated antigens as upconversion fluoroscent probes. Magnetic nanoparticles(MNPs) immobilized with monoclonal antibodies are used as immune-capture probes. Highly sensitive detection of FB1 and ZEN was achieved based on the luminescence properties of UCNPs and the separation effects of MNPs. The results showed a robust linear correlation between the enhanced fluorescence emission intensity and the logarithmic concentrations of FB1 and ZEN under the optimal conditions (R-2(FB1) = 0.9965, R-2(ZEN) = 0.9976), and the linear ranges were 05-5 ng mL(-1). Furthermore, the limits of detection (LOD) were 016 ng mL(-1) for FB1 and 012 ng mL(-1) for ZEN. The standard addition method was used to determine the content of FB1 and ZEN in the samples to evaluate the accuracy of the process. The average recoveries were 89.48% to 113.69% and 85.97% to 113.82%, respectively. Compared with the other five mycotoxins, this method had high selectivity. It is expected that the multi-component simultaneous detection can be further realized by using the multicolor labeling characteristics of UCNPs.
PublisherROYAL SOC CHEMISTRY
DOI10.1039/d1an00004g
Indexed ByEI ; SCOPUS ; SCIE
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000640674000001
Original Document TypeArticle
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.lzu.edu.cn/handle/262010/450309
Collection公共卫生学院
Corresponding AuthorHan, Tie
Affiliation1.Lanzhou Univ, Sch Publ Hlth, Lanzhou 730000, Peoples R China;
2.Tianjin Inst Environm & Operat Med, Tianjin Key Lab Risk Assessment & Control Technol, Tianjin 300050, Peoples R China
First Author AffilicationSchool of Public health
Recommended Citation
GB/T 7714
Li, Jingzhi,Zhao, Xudong,Wang, Yu,et al. A highly sensitive immunofluorescence sensor based on bicolor upconversion and magnetic separation for simultaneous detection of fumonisin B1 and zearalenone[J]. ANALYST,2021,146(10):3328-3335.
APA Li, Jingzhi.,Zhao, Xudong.,Wang, Yu.,Li, Shuang.,Qin, Yingkai.,...&Liu, Hui.(2021).A highly sensitive immunofluorescence sensor based on bicolor upconversion and magnetic separation for simultaneous detection of fumonisin B1 and zearalenone.ANALYST,146(10),3328-3335.
MLA Li, Jingzhi,et al."A highly sensitive immunofluorescence sensor based on bicolor upconversion and magnetic separation for simultaneous detection of fumonisin B1 and zearalenone".ANALYST 146.10(2021):3328-3335.
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