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Lipase immobilization on magnetic cellulose microspheres for rapid screening inhibitors from traditional herbal medicines
Liu, Jia1,2; Zhang, HX(张海霞)3; Shi, Yan-Ping1
2021-08-15
Source PublicationTalanta
ISSN00399140
Volume231
AbstractWith a dramatic increase in the incidence of obesity, it is significant to screen lipase inhibitors from traditional herbal medicines as drugs to treat obesity. Lipase inhibitors currently used to treat obesity possess the defects of toxicity and off-target effects. Thus, there is an urgent need to explore more safe, effective and targeted anti-obesity drugs from traditional herbal medicines. In this work, amino functionalized magnetic cellulose microsphere was employed as a novel support to immobilize lipase through covalent bonding. Characterizations from fourier transform infrared spectroscopy, transmission electron microscopy and X-ray diffraction demonstrated the successful preparation of the support. In comparison with the free lipase, the immobilized lipase manifested the excellent properties of a wider range for pH and temperature endurance, better pH, thermal, storage stability and reusability. Through investigating the kinetics performances of the immobilized lipase, the Michaelis-Menten constant was calculated to be 2.05 mM and its inhibition constant for orlistat was ascertained to be 40.74 μM. Eventually, the established strategy was applied to screen lipase inhibitors from 7 traditional herbal medicines and Crataegus pinnatifida Bunge was screened out due to its significant lipase inhibitory activity. To sum up, our newly established method not only developed a platform for efficiently discovering novel anti-obesity drugs from traditional herbal medicines, but also laid a solid foundation for successfully exploring undiscovered medicinal value of the traditional herbal medicines. © 2021 Elsevier B.V.
KeywordAnti-obesity Cellulose microspheres Crataegus pinnatifida bunge Herbal medicines Immobilized lipase Lipase immobilization Lipase inhibitor Magnetic cellulose microsphere Obesity drugs Traditional herbal medicine Anti-obesity Cellulose microspheres Crataegus pinnatifida bunge Herbal medicines Immobilized lipase Lipase immobilization Lipase inhibitor Magnetic cellulose microsphere Obesity drugs Traditional herbal medicine
PublisherElsevier B.V.
DOI10.1016/j.talanta.2021.122374
Indexed ByEI
Language英语
EI Accession Number20211510188377
EI KeywordsCellulose
EI Classification Number461.6 Medicine and Pharmacology ; 461.7 Health Care ; 461.9 Biology ; 701.2 Magnetism: Basic Concepts and Phenomena ; 741.3 Optical Devices and Systems ; 801 Chemistry ; 811.3 Cellulose, Lignin and Derivatives ; 815.1.1 Organic Polymers
Original Document TypeJournal article (JA)
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.lzu.edu.cn/handle/262010/451189
Collection化学化工学院
Affiliation1.CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicines of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou; 730000, China;
2.University of Chinese Academy of Sciences, Beijing; 100049, China;
3.College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou; 730000, China
Recommended Citation
GB/T 7714
Liu, Jia,Zhang, Hai-Xia,Shi, Yan-Ping. Lipase immobilization on magnetic cellulose microspheres for rapid screening inhibitors from traditional herbal medicines[J]. Talanta,2021,231.
APA Liu, Jia,Zhang, Hai-Xia,&Shi, Yan-Ping.(2021).Lipase immobilization on magnetic cellulose microspheres for rapid screening inhibitors from traditional herbal medicines.Talanta,231.
MLA Liu, Jia,et al."Lipase immobilization on magnetic cellulose microspheres for rapid screening inhibitors from traditional herbal medicines".Talanta 231(2021).
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