兰州大学机构库 >草地农业科技学院
Genome-wide profiling of the potential regulatory network of lncRNA and mRNA in Melilotus albus under salt stress
Zong, Xifang1; Wang, Shengsheng1; Han, Yangyang1; Zhao, Qiang2; Xu, Pan1; Yan, Qi1; Wu, Fan1; Zhang, JY(张吉宇)1
2021-09
Source PublicationENVIRONMENTAL AND EXPERIMENTAL BOTANY
ISSN0098-8472
Volume189
AbstractSalt stress is a major abiotic stress limiting crop productivity. Long noncoding RNAs (lncRNAs) are transcripts with more than 200 nucleotides but lacking protein-encoding potential and generally plays a regulatory role in plant response to abiotic stresses. However, no information is known about lncRNAs in Melilotus albus, a wonderful leguminous forage with excellent salt tolerance. In this study, 9437 lncRNAs were identified based on high-throughput sequencing from M. albus shoots and roots, most of which (7238, 76.7 %) were lincRNAs. Totally, 550 lncRNAs and 8273 mRNAs were differentially expressed in M. albus under salt stress, and lncRNA exhibited higher tissue-specificity in response to salt stress than mRNA. Five hundred and fifteen differentially expressed transcription factors were identified, many of which (AP2/ERF, MYB, bHLH, NAC) were related to abiotic stress. We predicted that 545 (99.1 %) differentially expressed lncRNAs (DELs) regulated 6616 (80.0 %) differentially expressed mRNA (DEGs) in cis, trans and miR-mediated manner. Numerous target genes of DELs were relevant to oxidation-reduction process, cellular protein metabolic process, cell communication, ribosome, carbon metabolism and biosynthesis of amino acid pathway. The competitive endogenous RNA (ceRNA) network contained 99 miRNA, 67 lncRNA, and 128 mRNA, among which qRT-PCR and tobacco dual-luciferase (LUC) assays further demonstrated that MSTRG.50852.2 might regulate the expression of EVM0029402 encoding WD40 domain protein by competitively binding mtr-miR7696a-3p under salt stress. Our results firstly characterize salt stress-responsive lncRNAs from M. ablus genome, which help to further research on the molecular mechanism of M. ablus adapting to salt stress and to provide useful information for resistance genetic improvement of other crops.
KeywordlncRNA Melilotus albus Salt tolerance Transcriptome sequencing ceRNA network
PublisherPERGAMON-ELSEVIER SCIENCE LTD
DOI10.1016/j.envexpbot.2021.104548
Indexed BySCOPUS ; SCIE
Language英语
WOS Research AreaPlant Sciences ; Environmental Sciences & Ecology
WOS SubjectPlant Sciences ; Environmental Sciences
WOS IDWOS:000671220500005
Original Document TypeArticle
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.lzu.edu.cn/handle/262010/451930
Collection草地农业科技学院
Affiliation1.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Minist Agr & Rural Affairs, State Key Lab Grassland Agroecosyst,Key Lab Grass, Lanzhou 730020, Peoples R China;
2.Heilongjiang Bayi Agr Univ, Daqing 163319, Peoples R China
First Author AffilicationCollege of Pastoral Agriculture Science and Technology
Recommended Citation
GB/T 7714
Zong, Xifang,Wang, Shengsheng,Han, Yangyang,et al. Genome-wide profiling of the potential regulatory network of lncRNA and mRNA in Melilotus albus under salt stress[J]. ENVIRONMENTAL AND EXPERIMENTAL BOTANY,2021,189.
APA Zong, Xifang.,Wang, Shengsheng.,Han, Yangyang.,Zhao, Qiang.,Xu, Pan.,...&Zhang, Jiyu.(2021).Genome-wide profiling of the potential regulatory network of lncRNA and mRNA in Melilotus albus under salt stress.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,189.
MLA Zong, Xifang,et al."Genome-wide profiling of the potential regulatory network of lncRNA and mRNA in Melilotus albus under salt stress".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 189(2021).
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