兰州大学机构库
A fluorescence aptasensor based on controlled zirconium-based MOFs for the highly sensitive detection of T-2 toxin
Zhao, Xudong1; Wang, Yu1; Li, Jingzhi1,2; Huo, Bingyang1,3; Qin, Yingkai1; Zhang, Jingyang1; Chen, Mengmeng1; Peng, Yuan1; Bai, Jialei1; Li, Shuang1; Gao, Zhixian1
2021-10-05
Source PublicationSPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
ISSN1386-1425
Volume259
AbstractT-2 toxin is one of class A trichothecene mycotoxins produced by Fusarium, presenting genotoxic, cytotoxicity, and immunotoxicity for animals and humans. Therefore, It is urgent to establish a rapid test method with high sensitivity, good selectivity and reliability. In this research, by adjusting the synthesis conditions, a kind of NH2-UiO-66 with high quenching efficiency was screened out. On this basis, we constructed a novel fluorescence sensor via Cy3-labeled aptamer (Cy3-aptamer). With the help of p-p interaction, hydrogen bond and coordination, NH2-UiO-66 could adsorb and quench the fluorescence of Cy3-aptamer based on FRET and PET. In the presence of T-2 toxin, it recognized and bound to Cy3-aptamer, leading to the disintegration of the NH2-UiO-66/Cy3-aptamer compound. As the energy transfer process was blocked, the fluorescence intensity was restored, enabling a highly sensitive response to T-2 toxin. There was a good linear correlation between fluorescence intensity and T-2 toxin concentration in the range of 0.5-100 ng ml(-1). The LOD of this fluorescence aptasensor was 0.239 ng ml(-1) (S/ N = 3). Besides, the recoveries of milk and beer were 89.86-108.99% (RSD = 2.0-2.6%) and 92.31111.51% (RSD = 2.3-2.9%), respectively. The fluorescence aptasensor exhibited advantages of excellent analytical performance, convenient operation procedure and good selectivity. Predictably, the aptasensor was supposed to detect antibiotics and other pollutants, describing an intriguing blueprint and potential application prospect in food safety, biochemical sensing and environmental conservation. (C) 2021 Elsevier B.V. All rights reserved.
KeywordT-2 toxin Fluorescence NH2-UiO-66 Controllable synthesis Aptasensor
PublisherPERGAMON-ELSEVIER SCIENCE LTD
DOI10.1016/j.saa.2021.119893
Indexed ByEI ; SCOPUS ; SCIE
Language英语
WOS Research AreaSpectroscopy
WOS SubjectSpectroscopy
WOS IDWOS:000656696100006
Original Document TypeArticle
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.lzu.edu.cn/handle/262010/452041
Collection兰州大学
Affiliation1.Tianjin Inst Environm & Operat Med, Tianjin Key Lab Risk Assessment & Control Technol, Tianjin 300050, Peoples R China;
2.Lanzhou Univ, Sch Publ Hlth, Lanzhou 730030, Peoples R China;
3.Sun Yat Sen Univ, Sch Chem, Guangzhou 510000, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Xudong,Wang, Yu,Li, Jingzhi,et al. A fluorescence aptasensor based on controlled zirconium-based MOFs for the highly sensitive detection of T-2 toxin[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2021,259.
APA Zhao, Xudong.,Wang, Yu.,Li, Jingzhi.,Huo, Bingyang.,Qin, Yingkai.,...&Gao, Zhixian.(2021).A fluorescence aptasensor based on controlled zirconium-based MOFs for the highly sensitive detection of T-2 toxin.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,259.
MLA Zhao, Xudong,et al."A fluorescence aptasensor based on controlled zirconium-based MOFs for the highly sensitive detection of T-2 toxin".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 259(2021).
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