兰州大学机构库 >化学化工学院
Ratiometric fluorescent detection and imaging of microRNA in living cells with manganese dioxide nanosheet-active DNAzyme
Zhang, Yuan1,2; Ma, Chunmeng1; Ma, Chen2; Xiang, Yueci1; Mu, Shuai1; Zheng, Zhixiang2; Liu, XY(刘晓燕)1; Zhang, HX(张海霞)1
2021-10-01
Source PublicationTALANTA
ISSN0039-9140
Volume233
AbstractMicroRNAs (miRNAs) play an important role in multiple biological processes and can be used as biomarkers for clinical disease diagnosis, so their detection is of great importance. Here, manganese dioxide (MnO2) nanosheet acts as carrier to deliver DNAzyme probes into cells through endocytosis, where intracellular glutathione (GSH) reduces the MnO2 nanosheet to manganese ions (Mn2+) and releases the probes. The generated Mn2+ can be further used as an effective cofactor to activate the DNAzyme probe, and cleave the DNA strand into two fragments. Then, the miRNA-155 in the cells can hybridize with the cleaved fragment to cause the fluorescence signal change of the probe. The proposed proportional fluorescent method has been applied to the imaging of miRNA-155 in HeLa cells and HepG2 cells with the estimated detection limit (LOD) as 1.6 x 10(-12) M. The new method can provide great help for cancer diagnosis and biological research related to miRNA.
KeywordManganese dioxide (MnO2) nanosheets DNAzyme miRNA-155 Fluorescence imaging
PublisherELSEVIER
DOI10.1016/j.talanta.2021.122518
Indexed ByEI ; SCOPUS ; SCIE
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000668000500042
Original Document TypeArticle
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.lzu.edu.cn/handle/262010/452336
Collection化学化工学院
Affiliation1.Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China;
2.Gansu Univ Polit Sci & Law, Key Lab Evidence Sci Tech Res & Applicat, Lanzhou 730070, Gansu, Peoples R China
First Author AffilicationCollege of Chemistry and Chemical Engineering
Recommended Citation
GB/T 7714
Zhang, Yuan,Ma, Chunmeng,Ma, Chen,et al. Ratiometric fluorescent detection and imaging of microRNA in living cells with manganese dioxide nanosheet-active DNAzyme[J]. TALANTA,2021,233.
APA Zhang, Yuan.,Ma, Chunmeng.,Ma, Chen.,Xiang, Yueci.,Mu, Shuai.,...&Zhang, Haixia.(2021).Ratiometric fluorescent detection and imaging of microRNA in living cells with manganese dioxide nanosheet-active DNAzyme.TALANTA,233.
MLA Zhang, Yuan,et al."Ratiometric fluorescent detection and imaging of microRNA in living cells with manganese dioxide nanosheet-active DNAzyme".TALANTA 233(2021).
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