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题名: Preparation of Magnetic Chitosan Nanoparticles As Support for Cellulase Immobilization
作者: Zang, LM; Qiu, JH; Wu, XL; Zhang, WJ; Sakai, E; Wei, Y
收录类别: SCIE ; EI
出版日期: 2014-03-05
刊名: INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
卷号: 53, 期号:9
出版者: AMER CHEMICAL SOC
出版地: WASHINGTON
英文摘要: A simple preparation process was developed for magnetic nanoparticles, consisting of chitosan coated on Fe3O4 nanoparticles, to be used as support for enzyme immobilization. Cellulase was covalently immobilized on this magnetic support using glutaraldehyde as a coupling agent. The structure, morphology, and magnetic property of the support were studied by X-ray diffraction, vibrating-sample magnetometer, thermogravimetric analysis, transmission electron microscopy, and Fourier transform infrared (FT-IR) spectroscopy. The properties of the immobilized cellulase were investigated by regarding activity, optimum operational pH and temperature, thermal stability, and reusability. The amount of cellulase on the nanoparticles reached 112.3 mg/g. The characterization and determination results showed that the immobilized cellulase had higher operational stability than the free enzyme over wider temperature and pH ranges and good reusability after recovery by magnetic separation. Therefore, these magnetic Fe3O4-chitosan nanoparticles are expected to be a useful support for enzyme.
作者部门: [Zang, Limin ; Qiu, Jianhui ; Wu, Xueli ; Zhang, Wenjuan ; Sakai, Eiichi] Akita Prefectural Univ, Dept Machine Intelligence & Syst Engn, Fac Syst Sci & Technol, Akita 0150055, Japan ; [Wei, Yi] Lanzhou Univ, Coll Chem & Chem Engn, Lanzhou 730000, Gansu, Peoples R China
通讯作者: Qiu, JH (reprint author), Akita Prefectural Univ, Dept Machine Intelligence & Syst Engn, Fac Syst Sci & Technol, Akita 0150055, Japan.
学科分类: Engineering
文章类型: Article
所属项目编号: Environment Research and Technology Development Fund of the Ministry of the Environment, Japan [3K113018]
语种: 英语
DOI: 10.1021/ie404072s
ISSN号: 0888-5885
WOS记录号: WOS:000332684500004
EI记录号: 20141117456990
第一机构:
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/74771
Appears in Collections:化学化工学院_期刊论文

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Recommended Citation:
Zang, LM; Qiu, JH; Wu, XL; Zhang, WJ; Sakai, E; Wei, Y.Preparation of Magnetic Chitosan Nanoparticles As Support for Cellulase Immobilization,INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,2014,53(9):
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