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兰州大学机构库  > 化学化工学院  > 期刊论文
题名: Biocatalytic Performance of pH-Sensitive Magnetic Nanoparticles Derived from Layer-by-Layer Ionic Self-Assembly of Chitosan with Glucoamylase
作者: Wang, JZ; Zhao, GH(赵光辉); Li, YF(李彦锋); Peng, XM; Li, YT
收录类别: SCIE ; EI ; PubMed ; MEDLINE
出版日期: 2013-12
刊名: CHEMISTRY-AN ASIAN JOURNAL
卷号: 8, 期号:12
出版者: WILEY
出版地: WEINHEIM
英文摘要: Based on the characteristics of polycations of chitosan and glucoamylase, which are oppositely charged, they were successfully alternatingly deposited onto the surface of aldehyde-modified Fe3O4 nanoparticles by using a layer-by-layer ion exchange method to form magnetic carriers to construct multilayer films (designated as Fe3O4@(CS/GA)(n)). The (CS/GA)(n) film systems were endowed with the pH-dependent properties of chitosan as well as the catalytic activity of glucoamylase. The changes in weight loss and surface chemistry, morphology, and magnetic sensitivity were monitored and verified by UV/Vis spectroscopy, zeta potential, TEM, and a vibrating sample magnetometer. Subsequently, the influence of the number of bilayers, storage stability, pH, temperature, and reusability of Fe3O4@(CS/GA)(5) biocatalysts on catalytic activity were investigated. The results from characterization and determination remarkably indicate that Fe3O4@(CS/GA)(5) presents excellent catalytic activity, storage stability, pH stability, and reusability in comparison with free enzyme. Fe3O4@(CS/GA)(5) retained >60% of its initial activity at 65 degrees C over 6h; the optimum temperature and pH also increased to the ranges of 45-65 degrees C and 2.5-3.5, respectively, and only 27% activity was lost after 10cycles. This new strategy simplifies the reaction protocol and improves encapsulation efficiency and catalytic activity for new potential applications in biotechnology.
关键词: cations ; enzymes ; magnetic properties ; self-assembly ; supported catalysts
作者部门: [Wang, Jian-zhi ; Zhao, Guang-hui ; Li, Yan-feng ; Peng, Xiao-meng ; Li, Yan-tao] Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China
通讯作者: Li, YF (reprint author), Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China.
学科分类: Chemistry
文章类型: Article
所属项目编号: National Natural Science Foundation of China [21074049] ; National Basic Science Foundation for Undergraduate Students [J1103307] ; Opening Foundation of State Key Laboratory of High Performance Civil Engineering Materials [2011CEM006] ; Fundamental Research Funds for the Central Universities [lzujbky-2012-75]
所属项目名称: 国家自然科学基金项目 ; 国家基础科学人才培养基金项目 ; 中央高校基本科研业务费专项资金
项目资助者: NSFC ; LZU
语种: 英语
DOI: 10.1002/asia.201300850
ISSN号: 1861-4728
WOS记录号: WOS:000327256100035
EI记录号: 20134917046940
PM记录号: 24273120
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内容类型: 期刊论文
URI标识: http://ir.lzu.edu.cn/handle/262010/74892
Appears in Collections:化学化工学院_期刊论文

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Wang, JZ; Zhao, GH; Li, YF; Peng, XM; Li, YT.Biocatalytic Performance of pH-Sensitive Magnetic Nanoparticles Derived from Layer-by-Layer Ionic Self-Assembly of Chitosan with Glucoamylase,CHEMISTRY-AN ASIAN JOURNAL,2013,8(12):
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