Microwave-assisted derivatization for fast and efficient analysis of saccharides on disposable microchips

doi:10.1039/c4ra07934e

兰州大学机构库 >化学化工学院

	Microwave-assisted derivatization for fast and efficient analysis of saccharides on disposable microchips
	Guo, JX; Yang, SH; Peng, XL; Li, FY(李风云); Zhou, L(周雷); Pu, QS(蒲巧生); Pu, QS (reprint author), Lanzhou Univ, Dept Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China.
	2014
Source Publication	RSC ADVANCES
ISSN	2046-2069
Volume	4 Issue:90 Pages:49190-49197
Abstract	For rapid and low-cost determination of saccharides with microchip electrophoresis, a domestic microwave oven was used to perform the derivatization of saccharides with 8-aminonaphthalene-1,3,6-trisulfonate (ANTS) for laser-induced fluorescence detection with a 405 nm laser diode. The influence of the reaction conditions was systematically examined. Derivatization of xylose, glucose and lactose was fulfilled within 3 min under the optimized condition. With appropriate selection of the separation medium, these model analytes could be resolved within 30 s in borate solutions containing hydroxypropyl cellulose using disposable cyclic olefin copolymer microchips. Theoretical plate numbers of 1.0 x 10(6) m(-1) and limits of detection of 0.15-0.23 mu M were achieved. Peak areas were linear up to 1000 mM for all analytes. The applicability of the proposed method was verified by the determination of glucose in human urine and serum. Separation of oligosaccharides from konjac powder was also demonstrated. The domestic microwave oven, the cheap laser diode and disposable microchips used here make the method an economical way for rapid analysis of saccharides.
Subject Area	Chemistry
Publisher	ROYAL SOC CHEMISTRY
DOI	10.1039/c4ra07934e
Publication Place	CAMBRIDGE
Indexed By	SCIE ; EI
Language	英语
First Inst	是
Funding Project	国家自然科学基金项目 ; 高等学校博士学科点专项科研基金 ; 中央高校基本科研业务费专项资金
Project Number	National Natural Science Foundation of China [21175062, 21105040] ; Specialized Research Fund for the Doctoral Program of Higher Education [20110211120010] ; Fundamental Research Funds for the Central Universities [lzujbky-2013-89]
WOS ID	WOS:000343711000057
Funding Organization	NSFC ; MOE ; LZU
Subtype	Article
EI ID	20144300122737
Department	[Guo, Jinxiu; Yang, Shenghong; Peng, Xianglu; Li, Fengyun; Zhou, Lei; Pu, Qiaosheng] Lanzhou Univ, Dept Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China
Citation statistics	Cited Times:3[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	https://ir.lzu.edu.cn/handle/262010/103296
Collection	化学化工学院
Corresponding Author	Pu, QS (reprint author), Lanzhou Univ, Dept Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China.
Recommended Citation GB/T 7714	Guo, JX,Yang, SH,Peng, XL,et al. Microwave-assisted derivatization for fast and efficient analysis of saccharides on disposable microchips[J]. RSC ADVANCES,2014,4(90):49190-49197.
APA	Guo, JX.,Yang, SH.,Peng, XL.,Li, FY.,Zhou, L.,...&Pu, QS .(2014).Microwave-assisted derivatization for fast and efficient analysis of saccharides on disposable microchips.RSC ADVANCES,4(90),49190-49197.
MLA	Guo, JX,et al."Microwave-assisted derivatization for fast and efficient analysis of saccharides on disposable microchips".RSC ADVANCES 4.90(2014):49190-49197.

Files in This Item:
File Name/Size	DocType	Version	Access	License
000343711000057.pdf（900KB）			开放获取	CC BY-NC-ND	View Application Full Text

File name:	000343711000057.pdf
Format:	Adobe PDF

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Institution:
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh

化学化工学院知识库