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Expression, purification and characterization of recombinant protein tyrosine phosphatase from Thermus thermophilus HB27
Wang, YJ; Meng, FG; Zhang, YM(张迎梅); Zhang, YM (reprint author), Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China.
2009-08
Source PublicationACTA BIOCHIMICA ET BIOPHYSICA SINICA   Impact Factor & Quartile
ISSN1672-9145
Volume41Issue:8Pages:689-698
AbstractThe low-molecular-weight protein tyrosine phosphatases (PTPase) exist ubiquitously in prokaryotes and eukaryotes and play important roles in the regulation of physiological activities. We report here the expression, purification and characterization of an active and soluble PTPase from Thermus thermophilus HB27 in Escherichia coli. This PTPase has an optimum pH range of 2.8-4.8 when using p-nitrophenyl phosphate as the substrate. The thermal inactivation results indicate a high thermal stability of this enzyme, with the optimum temperature of 75 degrees C for activity. It can be activated by Mn(2+), Mg(2+), Ca(2+), Ba(2+), and Ni(2+), but inhibited by Zn(2+), Cu(2+), Cl(-), and SO42-. These results suggest that this heat-resistant PTPase may play important roles in vivo in the adaptation of the microorganism to extreme temperatures and specic nutritional conditions.
Keywordprotein tyrosine phosphatase Thermus thermophilus HB27 characterization
Subject AreaBiochemistry & Molecular Biology ; Biophysics
PublisherOXFORD UNIV PRESS
DOI10.1093/abbs/gmp057
Publication PlaceOXFORD
Indexed BySCIE ; PubMed ; MEDLINE ; BIOSIS ; CSCD
Language英语
First Inst
Funding Project国家自然科学基金项目
Host of Journal中国科学院上海生科院生物化学与细胞生物学研究所
Project NumberQianjiang River Fellow Fund of Zhejiang Province [2007G50G3210005] ; National Natural Science Foundation of China [30770405]
WOS IDWOS:000268807900008
CSCD IDCSCD:3754705
Funding OrganizationNSFC
SubtypeArticle
PMID 19657570
BIOSIS IDBIOSIS:PREV200900522006
Department[Wang, Yejing;
Zhang, Yingmei] Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China;
[Meng, Fanguo] Tsinghua Univ, Yangtze Delta Reg Inst, Jiaxing 314050, Peoples R China
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/114738
Collection生命科学学院
Corresponding AuthorZhang, YM (reprint author), Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China.
Recommended Citation
GB/T 7714
Wang, YJ,Meng, FG,Zhang, YM,et al. Expression, purification and characterization of recombinant protein tyrosine phosphatase from Thermus thermophilus HB27[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2009,41(8):689-698.
APA Wang, YJ,Meng, FG,Zhang, YM,&Zhang, YM .(2009).Expression, purification and characterization of recombinant protein tyrosine phosphatase from Thermus thermophilus HB27.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,41(8),689-698.
MLA Wang, YJ,et al."Expression, purification and characterization of recombinant protein tyrosine phosphatase from Thermus thermophilus HB27".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 41.8(2009):689-698.
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